Evolution of a fluorinated green fluorescent protein

The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant containing 20 amino acid substitutions. The mutant protein in fluorinated form showed improved folding efficiency both in vivo and in vitro, and the median fluorescence of cells expressing the fluorinated protein was improved {approx}650-fold in comparison to that of cells expressing fluorinated GFPm. The success of this approach demonstrates the feasibility of engineering functional proteins containing many copies of abiological amino acid constituents

Antiferroquadrupolar Order in the Magnetic Semiconductor TmTe

The physical properties of the antiferroquadrupolar state occurring in TmTe below TQ=1.8 K have been studied using neutron diffraction in applied magnetic fields. A field-induced antiferromagnetic component k = (1/2,1/2,1/2) is observed and, from its magnitude and direction for different orientations of H, an O(2,2) quadrupole order parameter is inferred. Measurements below TN ~= 0.5 K reveal that the magnetic structure is canted, in agreement with theoretical predictions for in-plane antiferromagnetism. Complex domain repopulation effects occur when the field is increased in the ordered phases, with discontinuities in the superstructure peak intensities above 4 T.Comment: 6 pages, 6 figures, Presented at the International Conference on Strongly Correlated Electrons with Orbital Degrees of Freedom (ORBITAL 2001), September 11-14, 2001 (Sendai, JAPAN). To appear in: Journal of the Physical Society of Japan (2002

Measurements of thermodynamic and transport properties of EuC2_2: a low-temperature analogue of EuO

EuC$_2$ is a ferromagnet with a Curie-temperature of $T_C \simeq 15\,$K. It is semiconducting with the particularity that the resistivity drops by about 5 orders of magnitude on cooling through $T_C$, which is therefore called a metal-insulator transition. In this paper we study the magnetization, specific heat, thermal expansion, and the resistivity around this ferromagnetic transition on high-quality EuC$_2$ samples. At $T_C$ we observe well defined anomalies in the specific heat $c_p(T)$ and thermal expansion $\alpha(T)$ data. The magnetic contributions of $c_p(T)$ and $\alpha(T)$ can satisfactorily be described within a mean-field theory, taking into account the magnetization data. In zero magnetic field the magnetic contributions of the specific heat and thermal expansion fulfill a Gr\"uneisen-scaling, which is not preserved in finite fields. From an estimation of the pressure dependence of $T_C$ via Ehrenfest's relation, we expect a considerable increase of $T_C$ under applied pressure due to a strong spin-lattice coupling. Furthermore the influence of weak off stoichiometries $\delta$ in EuC$_{2 \pm \delta}$ was studied. It is found that $\delta$ strongly affects the resistivity, but hardly changes the transition temperature. In all these aspects, the behavior of EuC$_2$ strongly resembles that of EuO.Comment: 7 pages, 6 figure

Microscopic theory of quadrupolar ordering in TmTe

We have calculated the crystal electric field of TmTe (T>T_Q) and have obtained that the ground state of a Tm 4f hole is the $\Gamma_7$ doublet in agreement with Mossbauer experiments. We study the quadrupole interactions arising from quantum transitions of 4f holes of Tm. An effective attraction is found at the L point of the Brillouin zone, $\vec{q}_L$. Assuming that the quadrupolar condensation involves a single arm of $\vec{q}_L$ we show that there are two variants for quadrupole ordering which are described by the space groups C2/c and C2/m. The Landau free energy is derived in mean-field theory. The phase transition is of second order. The corresponding quadrupole order parameters are combinations of $T_{2g}$ and $E_g$ components. The obtained domain structure is in agreement with observations from neutron diffraction studies for TmTe. Calculated lattice distortions are found to be different for the two variants of quadrupole ordering. We suggest to measure lattice displacements in order to discriminate between those two structures.Comment: 10 pages, 2 figures, 5 tables; accepted by PR

Galactic Cosmic Ray Origins and OB Associations: Evidence from SuperTIGER Observations of Elements 26_{26}Fe through 40_{40}Zr

We report abundances of elements from $_{26}$Fe to $_{40}$Zr in the cosmic radiation measured by the SuperTIGER (Trans-Iron Galactic Element Recorder) instrument during 55 days of exposure on a long-duration balloon flight over Antarctica. These observations resolve elemental abundances in this charge range with single-element resolution and good statistics. These results support a model of cosmic-ray origin in which the source material consists of a mixture of 19$^{+11}_{-6}$\% material from massive stars and $\sim$81\% normal interstellar medium (ISM) material with solar system abundances. The results also show a preferential acceleration of refractory elements (found in interstellar dust grains) by a factor of $\sim$4 over volatile elements (found in interstellar gas) ordered by atomic mass (A). Both the refractory and volatile elements show a mass-dependent enhancement with similar slopes.Comment: 9 pages, 12 figures, 2 tables, accepted by Ap

Tkachenko waves, glitches and precession in neutron star

Here I discuss possible relations between free precession of neutron stars, Tkachenko waves inside them and glitches. I note that the proposed precession period of the isolated neutron star RX J0720.4-3125 (Haberl et al. 2006) is consistent with the period of Tkachenko waves for the spin period 8.4s. Based on a possible observation of a glitch in RX J0720.4-3125 (van Kerkwijk et al. 2007), I propose a simple model, in which long period precession is powered by Tkachenko waves generated by a glitch. The period of free precession, determined by a NS oblateness, should be equal to the standing Tkachenko wave period for effective energy transfer from the standing wave to the precession motion. A similar scenario can be applicable also in the case of the PSR B1828-11.Comment: 6 pages, no figures, accepted to Ap&S

Correlated decay of triplet excitations in the Shastry-Sutherland compound SrCu2_2(BO3_3)2_2

The temperature dependence of the gapped triplet excitations (triplons) in the 2D Shastry-Sutherland quantum magnet SrCu$_2$(BO$_3$)$_2$ is studied by means of inelastic neutron scattering. The excitation amplitude rapidly decreases as a function of temperature while the integrated spectral weight can be explained by an isolated dimer model up to 10~K. Analyzing this anomalous spectral line-shape in terms of damped harmonic oscillators shows that the observed damping is due to a two-component process: one component remains sharp and resolution limited while the second broadens. We explain the underlying mechanism through a simple yet quantitatively accurate model of correlated decay of triplons: an excited triplon is long-lived if no thermally populated triplons are near-by but decays quickly if there are. The phenomenon is a direct consequence of frustration induced triplon localization in the Shastry--Sutherland lattice.Comment: 5 pages, 4 figure

The Crustal Rigidity of a Neutron Star, and Implications for PSR 1828-11 and other Precession Candidates

We calculate the crustal rigidity parameter, b, of a neutron star (NS), and show that b is a factor 40 smaller than the standard estimate due to Baym & Pines (1971). For a NS with a relaxed crust, the NS's free-precession frequency is directly proportional to b. We apply our result for b to PSR 1828-11, a 2.5 Hz pulsar that appears to be precessing with period 511 d. Assuming this 511-d period is set by crustal rigidity, we show that this NS's crust is not relaxed, and that its reference spin (roughly, the spin for which the crust is most relaxed) is 40 Hz, and that the average spindown strain in the crust is 5 \times 10^{-5}. We also briefly describe the implications of our b calculation for other well-known precession candidates.Comment: 44 pages, 10 figures, submitted to Ap