Table_1_Lineage-level species distribution model to assess the impact of climate change on the habitat suitability of Boleophthalmus pectinirostris.docx

Global climate change has profound impacts on the habitats of marine organisms, and predicting the habitat changes of species under climate change conditions is crucial for species sustainability. Boleophthalmus pectinirostris is an intertidal fish species that holds significant ecological and economic value. To better protect and manage its resources, this study aimed to predict its current potential distribution and habitat changes under different climate scenarios in the future. This study firstly quantified the hypervolume niches of the three lineages (AE1, AE2, and AES lineages) and compared the niche differentiation among them. Furthermore, this study constructed species-level and lineage-level species distribution models (SDMs) to assess the impact of climate change on the habitat suitability of B. pectinirostris. The result of the niche differentiation assessment showed that there was marked differentiation in niches among the three lineages. The responses of different lineages to environmental variables were different, suggesting that lineage-level models may provide more accurate prediction results. According to the model predictions, the AES may have greater resilience to climate change and may experience habitat expansion in the future, while the AE1 and the AE2 may face habitat loss in some regions. Climate change-driven shifts in oceanic conditions were anticipated to affect the distribution and community structure of marine organisms. This study assessed the impact of climate change on the suitable habitat range of three lineages of B. pectinirostris using SDMs. Consistent with previous studies, the results of our study indicated that lineage-level SDMs may be more reliable than species-level SDMs for species with population differentiation in terms of the accuracy of predictions. In addition, considering the vulnerability of the AE1 and AE2 lineages to climate change, conserving these two lineages should be given a higher priority. The results of this study will provide important information for the future management and conservation of this species.</p

DataSheet_1_Lineage-level species distribution model to assess the impact of climate change on the habitat suitability of Boleophthalmus pectinirostris.docx

Global climate change has profound impacts on the habitats of marine organisms, and predicting the habitat changes of species under climate change conditions is crucial for species sustainability. Boleophthalmus pectinirostris is an intertidal fish species that holds significant ecological and economic value. To better protect and manage its resources, this study aimed to predict its current potential distribution and habitat changes under different climate scenarios in the future. This study firstly quantified the hypervolume niches of the three lineages (AE1, AE2, and AES lineages) and compared the niche differentiation among them. Furthermore, this study constructed species-level and lineage-level species distribution models (SDMs) to assess the impact of climate change on the habitat suitability of B. pectinirostris. The result of the niche differentiation assessment showed that there was marked differentiation in niches among the three lineages. The responses of different lineages to environmental variables were different, suggesting that lineage-level models may provide more accurate prediction results. According to the model predictions, the AES may have greater resilience to climate change and may experience habitat expansion in the future, while the AE1 and the AE2 may face habitat loss in some regions. Climate change-driven shifts in oceanic conditions were anticipated to affect the distribution and community structure of marine organisms. This study assessed the impact of climate change on the suitable habitat range of three lineages of B. pectinirostris using SDMs. Consistent with previous studies, the results of our study indicated that lineage-level SDMs may be more reliable than species-level SDMs for species with population differentiation in terms of the accuracy of predictions. In addition, considering the vulnerability of the AE1 and AE2 lineages to climate change, conserving these two lineages should be given a higher priority. The results of this study will provide important information for the future management and conservation of this species.</p

Table_2_Lineage-level species distribution model to assess the impact of climate change on the habitat suitability of Boleophthalmus pectinirostris.docx

Global climate change has profound impacts on the habitats of marine organisms, and predicting the habitat changes of species under climate change conditions is crucial for species sustainability. Boleophthalmus pectinirostris is an intertidal fish species that holds significant ecological and economic value. To better protect and manage its resources, this study aimed to predict its current potential distribution and habitat changes under different climate scenarios in the future. This study firstly quantified the hypervolume niches of the three lineages (AE1, AE2, and AES lineages) and compared the niche differentiation among them. Furthermore, this study constructed species-level and lineage-level species distribution models (SDMs) to assess the impact of climate change on the habitat suitability of B. pectinirostris. The result of the niche differentiation assessment showed that there was marked differentiation in niches among the three lineages. The responses of different lineages to environmental variables were different, suggesting that lineage-level models may provide more accurate prediction results. According to the model predictions, the AES may have greater resilience to climate change and may experience habitat expansion in the future, while the AE1 and the AE2 may face habitat loss in some regions. Climate change-driven shifts in oceanic conditions were anticipated to affect the distribution and community structure of marine organisms. This study assessed the impact of climate change on the suitable habitat range of three lineages of B. pectinirostris using SDMs. Consistent with previous studies, the results of our study indicated that lineage-level SDMs may be more reliable than species-level SDMs for species with population differentiation in terms of the accuracy of predictions. In addition, considering the vulnerability of the AE1 and AE2 lineages to climate change, conserving these two lineages should be given a higher priority. The results of this study will provide important information for the future management and conservation of this species.</p

Sakurai Reaction of 3,3-Bis(silyl) Silyl Enol Ethers with Acetals Involving Selective Desilylation of the Geminal Bis(silane). Concise Synthesis of Nematocidal Oxylipid

3,3-Bis(silyl) silyl enol ethers have been shown to exhibit predominantly Sakurai reactivity, rather than Mukaiyama aldol reactivity, in their Lewis acid promoted reactions with acetals. Starting from a geminal bis(silyl) moiety consisting of two different silyl groups, such as SiMe₃ and SiMe₂Ph, the SiMe₃ is selectively eliminated to give monoprotected <i>E</i>- vinylsilyl diols with good to excellent <i>syn</i>-diastereoselectivity. This reaction also underpinned a synthesis of the nematocidal oxylipid from <i>Notheia anomala</i>, demonstrating the attractive bifunctionality of geminal bis(silanes)

Regioselective Nucleophilic Addition of Organometallic Reagents to 3‑Geminal Bis(silyl) <i>N</i>‑Acyl Pyridinium

A regioselective nucleophilic addition to 3-geminal bis­(silyl) <i>N</i>-acyl pyridinium has been described. Geminal bis­(silane) shows contrasting roles that lead to different regioselectivities for the addition of different nucleophiles: its steric effect dominates to favor 1,6-addition of alkyl, vinyl, and aryl organometallic reagents; its directing effect dominates to favor 1,2-addition of less sterically demanding alkynyl Grignard reagents

Metal–Organic Framework Derivatives for Improving the Catalytic Activity of the CO Oxidation Reaction

Metal–organic framework (MOF)-based derivatives have attracted an increasing interest in various research fields. However, most of the reported papers mainly focus on pristine MOF-based derivatives, and research studies on functional MOF-based derivative composites are rare. Here, a simple strategy has been reported to design functional MOF-based derivative composites by the encapsulation of metal nanoparticle (MNP) in MOF matrixes (MNP@MOF) and the high-temperature calcination of MNP@MOF composites. The as-prepared MNP@metal oxide composites with a hierarchical pore structure exhibited excellent catalytic activity and high stability for the CO oxidation reaction

Fabrication of Flexible Transparent Electrode with Enhanced Conductivity from Hierarchical Metal Grids

Flexible transparent conductive electrodes (FTCEs) are essential components for numerous optoelectronic devices. In this work, we have fabricated the hierarchical metal grids (HMG) FTCEs by a facile and low-cost, near-field photolithography strategy. Compared to normal metal grids (MG), the HMG structure can provide distinctly increased conductivity of the electrode yet without obvious reduction of the optical transmittance. This HMG sample possesses excellent optoelectronic performance and high mechanical flexibility, making it a promising component for practical applications

Table_2_Spike 1 trimer, a nanoparticle vaccine against porcine epidemic diarrhea virus induces protective immunity challenge in piglets.XLSX

Porcine epidemic diarrhea virus (PEDV) is considered the cause for porcine epidemic diarrhea (PED) outbreaks and hefty losses in pig farming. However, no effective commercial vaccines against PEDV mutant strains are available nowadays. Here, we constructed three native-like trimeric candidate nanovaccines, i.e., spike 1 trimer (S1-Trimer), collagenase equivalent domain trimer (COE-Trimer), and receptor-binding domain trimer (RBD-Trimer) for PEDV based on Trimer-Tag technology. And evaluated its physical properties and immune efficacy. The result showed that the candidate nanovaccines were safe for mice and pregnant sows, and no animal death or miscarriage occurred in our study. S1-Trimer showed stable physical properties, high cell uptake rate and receptor affinity. In the mouse, sow and piglet models, immunization of S1-Trimer induced high-level of humoral immunity containing PEDV-specific IgG and IgA. S1-Trimer-driven mucosal IgA responses and systemic IgG responses exhibited high titers of virus neutralizing antibodies (NAbs) in vitro. S1-Trimer induced Th1-biased cellular immune responses in mice. Moreover, the piglets from the S1-Trimer and inactivated vaccine groups displayed significantly fewer microscopic lesions in the intestinal tissue, with only one and two piglets showing mild diarrhea. The viral load in feces and intestines from the S1-Trimer and inactivated vaccine groups were significantly lower than those of the PBS group. For the first time, our data demonstrated the protective efficacy of Trimer-Tag-based nanovaccines used for PEDV. The S1-Trimer developed in this study was a competitive vaccine candidate, and Trimer-Tag may be an important platform for the rapid production of safe and effective subunit vaccines in the future.</p

Data_Sheet_5_Spike 1 trimer, a nanoparticle vaccine against porcine epidemic diarrhea virus induces protective immunity challenge in piglets.PDF

Porcine epidemic diarrhea virus (PEDV) is considered the cause for porcine epidemic diarrhea (PED) outbreaks and hefty losses in pig farming. However, no effective commercial vaccines against PEDV mutant strains are available nowadays. Here, we constructed three native-like trimeric candidate nanovaccines, i.e., spike 1 trimer (S1-Trimer), collagenase equivalent domain trimer (COE-Trimer), and receptor-binding domain trimer (RBD-Trimer) for PEDV based on Trimer-Tag technology. And evaluated its physical properties and immune efficacy. The result showed that the candidate nanovaccines were safe for mice and pregnant sows, and no animal death or miscarriage occurred in our study. S1-Trimer showed stable physical properties, high cell uptake rate and receptor affinity. In the mouse, sow and piglet models, immunization of S1-Trimer induced high-level of humoral immunity containing PEDV-specific IgG and IgA. S1-Trimer-driven mucosal IgA responses and systemic IgG responses exhibited high titers of virus neutralizing antibodies (NAbs) in vitro. S1-Trimer induced Th1-biased cellular immune responses in mice. Moreover, the piglets from the S1-Trimer and inactivated vaccine groups displayed significantly fewer microscopic lesions in the intestinal tissue, with only one and two piglets showing mild diarrhea. The viral load in feces and intestines from the S1-Trimer and inactivated vaccine groups were significantly lower than those of the PBS group. For the first time, our data demonstrated the protective efficacy of Trimer-Tag-based nanovaccines used for PEDV. The S1-Trimer developed in this study was a competitive vaccine candidate, and Trimer-Tag may be an important platform for the rapid production of safe and effective subunit vaccines in the future.</p

Data_Sheet_1_Spike 1 trimer, a nanoparticle vaccine against porcine epidemic diarrhea virus induces protective immunity challenge in piglets.ZIP

Porcine epidemic diarrhea virus (PEDV) is considered the cause for porcine epidemic diarrhea (PED) outbreaks and hefty losses in pig farming. However, no effective commercial vaccines against PEDV mutant strains are available nowadays. Here, we constructed three native-like trimeric candidate nanovaccines, i.e., spike 1 trimer (S1-Trimer), collagenase equivalent domain trimer (COE-Trimer), and receptor-binding domain trimer (RBD-Trimer) for PEDV based on Trimer-Tag technology. And evaluated its physical properties and immune efficacy. The result showed that the candidate nanovaccines were safe for mice and pregnant sows, and no animal death or miscarriage occurred in our study. S1-Trimer showed stable physical properties, high cell uptake rate and receptor affinity. In the mouse, sow and piglet models, immunization of S1-Trimer induced high-level of humoral immunity containing PEDV-specific IgG and IgA. S1-Trimer-driven mucosal IgA responses and systemic IgG responses exhibited high titers of virus neutralizing antibodies (NAbs) in vitro. S1-Trimer induced Th1-biased cellular immune responses in mice. Moreover, the piglets from the S1-Trimer and inactivated vaccine groups displayed significantly fewer microscopic lesions in the intestinal tissue, with only one and two piglets showing mild diarrhea. The viral load in feces and intestines from the S1-Trimer and inactivated vaccine groups were significantly lower than those of the PBS group. For the first time, our data demonstrated the protective efficacy of Trimer-Tag-based nanovaccines used for PEDV. The S1-Trimer developed in this study was a competitive vaccine candidate, and Trimer-Tag may be an important platform for the rapid production of safe and effective subunit vaccines in the future.</p