Digestion of human serum albumin by the recombinant human scabies mite aspartic protease N23SsAP.

<p>Recombinant N23SsAP was tested for its ability to digest human serum albumin. Reactions were run on a 15% non-reducing SDS-PAGE gel and stained with coomassie brilliant blue R-250. Lane 1, molecular weight marker; lanes 2–6, serum albumin plus N23SsAP, incubated for 15 minutes (lane 2), 1 hour (lane 3), 2 hours (lane 4), 4 hours (lane 5) or 12 hours (lane 6).</p

Digestion of human fibronectin by the recombinant human scabies mite aspartic protease N23SsAP.

<p>Recombinant N23SsAP was tested for its ability to digest human fibronectin. Reactions were run on a 15% non-reducing SDS-PAGE gel and stained with coomassie brilliant blue R-250. Lane 1, molecular weight marker; lane 2, fibrinonectin alone; lanes 3 and 5, fibrinonectin plus N23SsAP; lanes 4 and 6, fibrinonectin plus N23SsAP and pepstatin A, after 4 hours incubation (lanes 3 and 4) and 13 hours incubation (lanes 5 and 6).</p

Digestion of human fibrinogen by the recombinant human scabies mite aspartic protease N23SsAP.

<p>Recombinant N23SsAP was tested for its ability to digest human fibrinogen. Reactions were run on a 15% non-reducing SDS-PAGE gel and stained with coomassie brilliant blue R-250. Lane 1, molecular weight marker; lane 2, fibrinogen alone; lanes 3 and 5, fibrinogen plus N23SsAP; lanes 4 and 6, fibrinogen plus N23SsAP and pepstatin A, after 4 hours incubation (lanes 3 and 4) and 13 hours incubation (lanes 5 and 6).</p

Digestion of human collagen III by the recombinant human scabies mite aspartic protease N23SsAP.

<p>Recombinant N23SsAP was tested for its ability to digest human collagen III. Reactions were run on a 15% non-reducing SDS-PAGE gel and stained with coomassie brilliant blue R-250. Lane 1, molecular weight marker; lane 2, collagen III alone; lanes 3 and 5, collagen III plus N23SsAP; lanes 4 and 6, collagen III plus N23SsAP and pepstatin A, after 4 hours incubation (lanes 3 and 4) and 13 hours incubation (lanes 5 and 6).</p

Digestion of human haemoglobin by the recombinant human scabies mite aspartic protease N23SsAP.

<p>The haemoglobinolytic activity of N23SsAP was detected using native human haemoglobin as substrate at pH% non-reducing SDS-PAGE gel and stained with coomassie brilliant blue R-250. Lane 1, molecular weight marker; lane 2, haemoglobin alone; lanes 3–6, haemoglobin plus N23SsAP, incubated for 1 hour (lane 3), 2 hours (lane 4), 4 hours (lane 5) or 12 hours (lane 6).</p

Detection of aspartic protease activity in scabies mite extract using a fluorescent substrate.

<p>The fluorescence signal was recorded at 60 second intervals, commencing after 10 minutes.</p