Sodium dodecyl sulfate assisted synthesis hydroxyapatite for effective adsorption of methylene blue

Plate-shaped, hollow flower-shaped, and flower-clustered hydroxyapatite (HAP) were prepared with CaCl2 and (NH4)2HPO4 as raw materials and with sodium dodecyl sulfate (SDS) as additives. XRD, SEM, BET, and FT-IR were used to characterize the prepared HAP. The adsorption performance of methylene blue (MB) on HAP was investigated. The results showed that adding SDS to an appropriate amount increased the dispersibility and specific surface area of HAP. There is an electrostatic attraction between the surface of HAP and SDS, exposing an increasing number of negatively charged phosphate groups, making the adsorption capacity of cationic dye MB to be improved. SDS-HAP has a removal efficiency of more than 95% of MB under different pH and concentration conditions. The maximum saturated adsorption capacity reached 468.65 mg/g. After three adsorption-desorption cycles, the removal rate of methylene blue is still above 85%. The adsorption of MB by SDS-HAP is an exothermic physical process and conforms to the Langmuir model and the pseudo-second-order kinetic model.</p

Robust Superamphiphobic Coating Applied to Grease-Proof Mining Transformer Components

The iron core and heat sink in a mining transformer are susceptible to damage from oil spills or the harsh mine environment; the deterioration of oil products in the underground environment and transformers produce massive amounts of harmful liquid substances, which may lead to unnecessary economic losses in drilling engineering. To overcome this issue, a convenient and economical way to protect transformer components was developed. Herein, we proposed an air spray technology at room temperature for the preparation of antigreasy superamphiphobic coatings, which are suitable for bulk metallic glass transformer cores and ST13 heat sinks. The addition of polypyrrole powder effectively improves the thermal conductivity and specific heat of the coating in the range of 50–70 °C. More importantly, the fabricated coating has excellent repellency to liquids, such as water, ethylene glycerol, hexadecane, and rapeseed oil. Meanwhile, the coating has excellent physical and chemical resistance and outstanding antifouling features, which provide a feasible solution for combating grease pollution and corrosion in the mine environment. Taking multifaceted stability into consideration, this work contributes to enhancing the application of superamphiphobic coatings in the fields of protecting transformer components in the harsh environment or during transformer operation faults

Additional file 2 of Dysregulated long noncoding RNAs in the brainstem of the DBA/1 mouse model of SUDEP

Additional file 2. Details of the corresponding probe and region of each lincRN

<i>Lactobacillus acidophilus</i> attenuated TBI-mediated increase of MLCP.

<p>The interaction of <i>Lactobacillus acidophilus</i> and time on MLCP protein concentrations was not significant. (A) ELISA analysis showed that the concentrations of MLCP in intestinal smooth muscle were significantly increased after TBI, **<i>P</i> < 0.01 compared with control. <i>Lactobacillus acidophilus</i> significantly attenuated TBI-mediated increase of MLCP, #<i>P</i> < 0.05 compared with TBI. (B) Immunohistochemistry analysis showed that marked increases in MLCP were observed in intestinal smooth muscle after TBI, and treatment with <i>Lactobacillus acidophilus</i> significantly attenuated TBI-mediated increases of MLCP.</p

Dual Gold Nanoparticle/Chemiluminescent Immunoassay for Sensitive Detection of Multiple Analytes

Multiple antibiotics and mycotoxins usually simultaneously exist in foods, which poses a serious threat to human health. How to detect them in one test with high sensitivity and fidelity is challenging. In this study, we develop a dual readout lateral flow immunodetection platform that can quantitatively detect five kinds of antibiotics and five kinds of mycotoxins within one sample. The platform is composed of a chip and a portable readout instrument where gold nanoparticle (AuNP)-based and chemiluminescence immunoassays could be performed to reach a maximum throughput of 220 analytes in one setting. For a rapid screen, qualitative analysis by detecting the color change of the deposited AuNPs on the chip could be realized. For quantitative results, chemiluminescence imaging and analysis can be completed within 15 min. Apart from the high throughput and high efficiency, this platform has a high detection sensitivity. For instance, the limit of detection (LOD) for thiamphenicol (a representative antibiotic) and fumonisins B1 (a representative mycotoxin) is 8 times and 40 times lower than those of the previously reported methods, respectively. Thus, this dual readout immunodetection platform is promising as a universal device for rapid and quantitative detection of multiple analytes with high throughput, high sensitivity, and high fidelity

Heterogeneous Iron-Catalyzed Aerobic Oxidative Cleavage of C–C Bonds in Alcohols to Esters

A heterogeneous iron-based catalyst for the aerobic oxidative cleavage of C–C bonds in alcohols to access esters was developed. Various alcohols including inactive long-chain alkyl aryl alcohols as well as β-O-4 lignin model compounds can be smoothly converted to the corresponding esters with molecular oxygen as the oxidant. Moreover, the present catalyst system can successfully degrade organosolv lignin. Base additives are not required except for inactive long-chain alkyl aryl alcohols. The catalyst exhibits good recyclability and can be easily recycled and reused up to seven times without a significant loss of catalytic activity. Characterization and control experiments confirmed that iron nanoparticles are mainly responsible for this reaction. Preliminary mechanism studies indicate that a sequential oxidation process exists in the reaction pathway

Additional file 1 of Dysregulated long noncoding RNAs in the brainstem of the DBA/1 mouse model of SUDEP

Additional file 1: Table 1. Differentially expressed antisense lncRNAs and nearby coding gene. *The SUDEP group compared with normal group. Table 2. Differentially expressed lincRNAs and adjacent mRNAs. lincRNAs, long intergenic noncoding RNAs.*The SUDEP group compared with normal group

Dual Gold Nanoparticle/Chemiluminescent Immunoassay for Sensitive Detection of Multiple Analytes

Multiple antibiotics and mycotoxins usually simultaneously exist in foods, which poses a serious threat to human health. How to detect them in one test with high sensitivity and fidelity is challenging. In this study, we develop a dual readout lateral flow immunodetection platform that can quantitatively detect five kinds of antibiotics and five kinds of mycotoxins within one sample. The platform is composed of a chip and a portable readout instrument where gold nanoparticle (AuNP)-based and chemiluminescence immunoassays could be performed to reach a maximum throughput of 220 analytes in one setting. For a rapid screen, qualitative analysis by detecting the color change of the deposited AuNPs on the chip could be realized. For quantitative results, chemiluminescence imaging and analysis can be completed within 15 min. Apart from the high throughput and high efficiency, this platform has a high detection sensitivity. For instance, the limit of detection (LOD) for thiamphenicol (a representative antibiotic) and fumonisins B1 (a representative mycotoxin) is 8 times and 40 times lower than those of the previously reported methods, respectively. Thus, this dual readout immunodetection platform is promising as a universal device for rapid and quantitative detection of multiple analytes with high throughput, high sensitivity, and high fidelity

DataSheet1_A Novel Variant of the CHD2 Gene Associated With Developmental Delay and Myoclonic Epilepsy.zip

Pathogenic variants in CHD2 have been reported to have a wide range of phenotypic variability in neurodevelopmental disorders, such as early-onset epileptic encephalopathy, developmental delay, and behavior problems. So far, there is no clear correlation between genotypes and phenotypes. This study reports a Chinese patient with a novel heterozygous CHD2 mutation (c.4318C>T, pArg1440*). Her main clinical manifestations include developmental delay, myoclonic epilepsy, and hypothyroidism. Then, we reviewed a total of 144 individuals carrying CHD2 variants with epileptic encephalopathy. In terms of clinical manifestations, these patients are usually described with variable epilepsy phenotypes, including idiopathic photosensitive occipital epilepsy, Dravet syndrome, Jeavons syndrome, Lennox–Gastaut syndrome, juvenile myoclonic epilepsy, and non-specific epileptic encephalopathy. Among them, myoclonic seizures and generalized tonic-clonic seizures are the main seizure types in all patients hosting CHD2 single-nucleotide or indel variants (non-CNVs). At the molecular level, there are 102 types of CHD2 non-CNVs in 126 patients, almost one mutational type corresponding to one person, and there is no difference in the incidence ratio of each position. Furthermore, we summarized that a small proportion of patients inherited CHD2 variants, and not all patients with CHD2 variants had seizures. Importantly, the phenotypes, especially seizures control and fever sensitivity, and genotypes had a relative association. These results enriched the database of CHD2-relative neurodevelopmental disorders and provided a theoretical foundation for researching the relationship between genotypes and phenotypes.</p

<i>P</i>-values of a two-way ANOVA for the effects of <i>Lactobacillus acidophilus</i>, time, and their interactions on the ten variables (N = 90).

<p>Note: MLCK: myosin light chain kinase; MLCP: myosin light chain phosphatase; PKC: protein kinase C; ICC numbers: the numbers of interstitial cells of Cajal.</p><p>*indicates a significant difference (<i>P</i> < 0.05)</p><p>**indicates a highly significant difference (<i>P</i> < 0.01).</p><p><i>P</i>-values of a two-way ANOVA for the effects of <i>Lactobacillus acidophilus</i>, time, and their interactions on the ten variables (N = 90).</p