Hyperbranched Tetraphenylethylene Derivatives with Low Non-specific Aggregation-Induced Emission for Fluorescence Recognition of Proteins with Hydrophobic Pockets

Proteins play an important role in the physiological process of many organisms, and their abnormal level often indicates the occurrence of some diseases. Therefore, protein analysis has important reference value and clinical significance for early diagnosis and therapy of disease. Using human serum albumin (HSA) as a model protein, a series of super-branched tetraphenylethylene (TPE) derivatives with different branching structures and terminal groups are reported herein for highly sensitive and specific recognition of proteins with hydrophobic cages. Benefiting from the hyperbranched structures, these probes showed much higher critical micelle concentrations (CMCs) than most linear TPE-based amphiphilic molecules since the hyperbranched structure not only improved their solubility but also amplified the steric hindrance effect and electrostatic repulsive force to prevent their aggregation. Dynamic light scattering experiments proved that these probes formed dense aggregates at CMC, and such aggregate structures would lead to a higher background fluorescence noise. Hence, a higher CMC is more conducive to the detection of the target with low backgrounds. Among them, P3-COOH with −COOH as the terminal unit and a relatively longer branch showed the highest CMC and the best signal to background ratio (S/N). Mechanism studies showed that P3-COOH was bound to HSA mainly through a hydrophobic force, resulting in a limited P3-COOH molecular movement and less attack from quenchers in solutions, thus leading to greatly enhanced fluorescence intensity. In addition, P3-COOH was also applied to the determination of HSA content in actual human serum samples

Dual-Functional WO₃ Nanocolumns with Broadband Antireflective and High-Performance Flexible Electrochromic Properties

The three-dimensional, high-porous, and oriented WO₃ nanocolumn film with broadband antireflective and high-performance flexible electrochromic dual-functionalities is achieved by utilizing a simple, one-step, room-temperature glancing angle deposition without any catalysts and templates. It is found that the WO₃ nanocolumn film is effective in increasing the optical transparency in the visible range, enhancing the color-switching response time as well as improving the mechanical flexibility and electrochemical cycling stability in comparison to dense WO₃ film. The further optical, morphological, and electrode reaction kinetics analyses reveal that these improvements can be attributed to its unique porous nanocolumn arrays, which reduce the refractive index, facilitate the interfacial charge-transfer and ion-penetration, and alleviate the internal stress of the film under the bending treatment. These results would provide a simple and effective guidance to design and construct low-cost, robust, flexible, stable, and transparent electrochromic smart windows

Bifunctional MoO₃–WO₃/Ag/MoO₃–WO₃ Films for Efficient ITO–Free Electrochromic Devices

Dielectric–metal–dielectric (DMD) trilayer films, served as both electrochromic (EC) film and transparent conductor (TC), have exhibited great potential application in low–cost, ITO–free electrochromic devices (ECDs). However, recent reports on the DMD–based ECDs revealed that the response time and the optical modulation properties were not very satisfactory. Here, the mixed MoO₃–WO₃ materials were first introduced as the dielectric layer to construct an EC–TC bifunctional MoO₃–WO₃/Ag/MoO₃–WO₃ (MWAMW) film, which demonstrates strong and broad–band optical modulation in the visible light region, fast color-switching time (2.7 s for coloration and 4.1 s for bleaching), along with high coloration efficiency (70 cm² C^–1). The electrical structure and electrochemical reaction kinetics analysis revealed that the improved EC performances are associated with the increased electron intervalence transition together with the fast charge–transfer and ion–diffusion dynamics

Data_Sheet_1_Lysosomal-Associated Protein Transmembrane 5 Functions as a Novel Negative Regulator of Pathological Cardiac Hypertrophy.PDF

Lysosomal-associated protein transmembrane 5 (LAPTM5) is mainly expressed in immune cells and has been reported to regulate inflammation, apoptosis and autophagy. Although LAPTM5 is expressed in the heart, whether LAPTM5 plays a role in regulating cardiac function remains unknown. Here, we show that the expression of LAPTM5 is dramatically decreased in murine hypertrophic hearts and isolated hypertrophic cardiomyocytes. In this study, we investigated the role of LAPTM5 in pathological cardiac hypertrophy and its possible mechanism. Our results show that LAPTM5 gene deletion significantly exacerbates cardiac remodeling, which can be demonstrated by reduced myocardial hypertrophy, fibrosis, ventricular dilation and preserved ejection function, whereas the opposite phenotype was observed in LAPTM5 overexpression mice. In line with the in vivo results, knockdown of LAPTM5 exaggerated angiotensin II-induced cardiomyocyte hypertrophy in neonatal rat ventricular myocytes, whereas overexpression of LAPTM5 protected against angiotensin II-induced cardiomyocyte hypertrophy in vitro. Mechanistically, LAPTM5 directly bound to Rac1 and further inhibited MEK-ERK1/2 signaling, which ultimately regulated the development of cardiac hypertrophy. In addition, the antihypertrophic effect of LAPTM5 was largely blocked by constitutively active mutant Rac1 (G12V). In conclusion, our results suggest that LAPTM5 is involved in pathological cardiac hypertrophy and that targeting LAPTM5 has great therapeutic potential in the treatment of pathological cardiac hypertrophy.</p

Data_Sheet_2_Lysosomal-Associated Protein Transmembrane 5 Functions as a Novel Negative Regulator of Pathological Cardiac Hypertrophy.XLSX

Lysosomal-associated protein transmembrane 5 (LAPTM5) is mainly expressed in immune cells and has been reported to regulate inflammation, apoptosis and autophagy. Although LAPTM5 is expressed in the heart, whether LAPTM5 plays a role in regulating cardiac function remains unknown. Here, we show that the expression of LAPTM5 is dramatically decreased in murine hypertrophic hearts and isolated hypertrophic cardiomyocytes. In this study, we investigated the role of LAPTM5 in pathological cardiac hypertrophy and its possible mechanism. Our results show that LAPTM5 gene deletion significantly exacerbates cardiac remodeling, which can be demonstrated by reduced myocardial hypertrophy, fibrosis, ventricular dilation and preserved ejection function, whereas the opposite phenotype was observed in LAPTM5 overexpression mice. In line with the in vivo results, knockdown of LAPTM5 exaggerated angiotensin II-induced cardiomyocyte hypertrophy in neonatal rat ventricular myocytes, whereas overexpression of LAPTM5 protected against angiotensin II-induced cardiomyocyte hypertrophy in vitro. Mechanistically, LAPTM5 directly bound to Rac1 and further inhibited MEK-ERK1/2 signaling, which ultimately regulated the development of cardiac hypertrophy. In addition, the antihypertrophic effect of LAPTM5 was largely blocked by constitutively active mutant Rac1 (G12V). In conclusion, our results suggest that LAPTM5 is involved in pathological cardiac hypertrophy and that targeting LAPTM5 has great therapeutic potential in the treatment of pathological cardiac hypertrophy.</p

Additional file 1: of Association of plasma soluble CD14 level with asthma severity in adults: a case control study in China

Table S1. The Severity Scale of asthma patients in the Guidelines for Prevention and Treatment of Bronchial Asthma in China. (DOC 37 kb

Data_Sheet_2_Relative Weights of Temporal Envelope Cues in Different Frequency Regions for Mandarin Vowel, Consonant, and Lexical Tone Recognition.docx

Objectives: Mandarin-speaking users of cochlear implants (CI) perform poorer than their English counterpart. This may be because present CI speech coding schemes are largely based on English. This study aims to evaluate the relative contributions of temporal envelope (E) cues to Mandarin phoneme (including vowel, and consonant) and lexical tone recognition to provide information for speech coding schemes specific to Mandarin.Design: Eleven normal hearing subjects were studied using acoustic temporal E cues that were extracted from 30 continuous frequency bands between 80 and 7,562 Hz using the Hilbert transform and divided into five frequency regions. Percent-correct recognition scores were obtained with acoustic E cues presented in three, four, and five frequency regions and their relative weights calculated using the least-square approach.Results: For stimuli with three, four, and five frequency regions, percent-correct scores for vowel recognition using E cues were 50.43–84.82%, 76.27–95.24%, and 96.58%, respectively; for consonant recognition 35.49–63.77%, 67.75–78.87%, and 87.87%; for lexical tone recognition 60.80–97.15%, 73.16–96.87%, and 96.73%. For frequency region 1 to frequency region 5, the mean weights in vowel recognition were 0.17, 0.31, 0.22, 0.18, and 0.12, respectively; in consonant recognition 0.10, 0.16, 0.18, 0.23, and 0.33; in lexical tone recognition 0.38, 0.18, 0.14, 0.16, and 0.14.Conclusion: Regions that contributed most for vowel recognition was Region 2 (502–1,022 Hz) that contains first formant (F1) information; Region 5 (3,856–7,562 Hz) contributed most to consonant recognition; Region 1 (80–502 Hz) that contains fundamental frequency (F0) information contributed most to lexical tone recognition.</p

Additional file 1: of Association of lung function with cardiovascular risk: a cohort study

Table S1. Ten-Year CVD Risk by GOLD classification and selected baseline characteristics in COPD patients (N = 101). (DOCX 17 kb

Table_1_Thyroid-Related Hormone Levels in Clinical Patients With Moderately Severe-to-Profound Sudden Sensorineural Hearing Loss: A Prospective Study.DOCX

Objectives: Sudden sensorineural hearing loss (SSNHL) is a common otological emergency, causing a measure of hearing loss and affecting the quality of life. This study aims to investigate the association of thyroid-related hormone levels with moderately severe-to-profound SSNHL.Methods: The study included 70 patients with moderately severe-to-profound SSNHL and 100 age- and sex-matched healthy controls. Peripheral venous blood samples were taken from the participants, and their thyroid-related hormone levels were measured at admission and 1 week after treatment.Results: In moderately severe-to-profound SSNaHL patients, the concentrations of total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3), and thyroid-stimulating hormone (TSH) (all P Conclusions: The results suggest that low FT3 and TSH levels are risk factors for moderately severe-to-profound SSNHL. By discovering the positive association between TSH elevation and hearing recovery, along with the potential novel predictors of FT3 and TSH, our study may contribute valuable insights to the research and treatment of moderately severe-to-profound SSNHL.</p

Data_Sheet_1_Relative Weights of Temporal Envelope Cues in Different Frequency Regions for Mandarin Vowel, Consonant, and Lexical Tone Recognition.XLSX

Objectives: Mandarin-speaking users of cochlear implants (CI) perform poorer than their English counterpart. This may be because present CI speech coding schemes are largely based on English. This study aims to evaluate the relative contributions of temporal envelope (E) cues to Mandarin phoneme (including vowel, and consonant) and lexical tone recognition to provide information for speech coding schemes specific to Mandarin.Design: Eleven normal hearing subjects were studied using acoustic temporal E cues that were extracted from 30 continuous frequency bands between 80 and 7,562 Hz using the Hilbert transform and divided into five frequency regions. Percent-correct recognition scores were obtained with acoustic E cues presented in three, four, and five frequency regions and their relative weights calculated using the least-square approach.Results: For stimuli with three, four, and five frequency regions, percent-correct scores for vowel recognition using E cues were 50.43–84.82%, 76.27–95.24%, and 96.58%, respectively; for consonant recognition 35.49–63.77%, 67.75–78.87%, and 87.87%; for lexical tone recognition 60.80–97.15%, 73.16–96.87%, and 96.73%. For frequency region 1 to frequency region 5, the mean weights in vowel recognition were 0.17, 0.31, 0.22, 0.18, and 0.12, respectively; in consonant recognition 0.10, 0.16, 0.18, 0.23, and 0.33; in lexical tone recognition 0.38, 0.18, 0.14, 0.16, and 0.14.Conclusion: Regions that contributed most for vowel recognition was Region 2 (502–1,022 Hz) that contains first formant (F1) information; Region 5 (3,856–7,562 Hz) contributed most to consonant recognition; Region 1 (80–502 Hz) that contains fundamental frequency (F0) information contributed most to lexical tone recognition.</p