Additional file 1 of A new approach to enter Retzius space in laparoscopic transabdominal preperitoneal bilateral inguinal hernia repair

Additional file 1: The raw data

Additional file 1 of A hsa_circ_001726 axis regulated by E2F6 contributes to metastasis of hepatocellular carcinoma

Additional file 1:Supplementary Fig. 1. Detection of transcription efficiency. (A) QRT-PCR assessed the expression of hsa_circ_001726 in MHCC97H and Huh7 cells following transfection of si-hsa_circ_001726 or si-NC. (B) QRT-PCR detected the expression of hsa_circ_001726 in Huh7 cells following transfection of LV-sh-hsa_circ_001726 or LV-sh-NC. (C) QRT-PCR assessed the expression of miR-671-5p in MHCC97H and Huh7 cells following transfection of miR-671-5p mimic or mimic NC. (D) QRT-PCR examined the expression of miR-671-5p in MHCC97H and Huh7 cells following transfection of miR-671-5p-In or In-NC. (E-F) QRT-PCR and western blotting examined the expression of PRMT9 in MHCC97H and Huh7 cells following transfection of PRMT9-OE or Vector. (G-H) QRT-PCR and western blotting assessed the expression of E2F6 in Huh7 and MHCC97H cells following of si-E2F6 and si-NC. *P < 0.05, **P < 0.01 vs si-NC, shNC, mimic-NC, In-NC, Vector group. Supplementary Fig. 2. Hsa_circ_001726 knockdown alleviated lung metastasis in orthotopic transplantation tumor model. Orthotopic transplantation tumor model was constructed by inoculating Huh7 cells with LV-sh-hsa_circ_001726 or Huh7 cells with LV-sh-NC. HE staining examined the pathological changes of lung tissues. The full scan of HE staining. Supplementary Fig. 3. MiR-671-5p overexpression reduced migration and invasion of MHCC97H and Huh7 cells. (A-B) Wound healing and Transwell invasion assays examined migration and invasion of MHCC97H and Huh7 cells following transfection of miR-671-5p mimic or mimic NC. **P < 0.01 vs mimic-NC group

Video_1_Case Report: Transvaginal specimen extraction following totally laparoscopic D2 distal gastrectomy for gastric cancer in a patient with situs inversus totalis: with video.mp4

Because of its significant advantage of fast postoperative recovery, natural orifice specimen extraction surgery (NOSES) has attracted increasing attention worldwide. However, the NOSES in gastric cancer (GC) treatment still needs more clinical practice, especially for the rare anatomical anomaly. Situs inversus totalis (SIT) is a rare autosomal recessive anatomical anomaly with an incidence ranging between 1/8,000 and 1/25,000 births. We present a video of transvaginal specimen extraction following totally laparoscopic D2 distal gastrectomy performed in a 59-year-old woman known to have SIT. Preoperative investigations revealed that the patient had early GC at the antrum. A gastroscopy report from the local hospital showed signet-ring cell carcinoma. The preoperative computed tomography scan revealed irregular thickening of the gastric wall at the junction of the greater curvature and antrum without metastasis to the lymph nodes. In total, laparoscopic D2 distal gastrectomy was performed with transvaginal specimen extraction. Billroth II with Braun anastomosis was performed for reconstruction. The length of the operation was 240 min without intraoperative complications and with minimal blood loss of 50 ml. The patient was uneventfully discharged on postoperative Day 7. The final pathology confirmed signet-ring cell carcinoma confined to the mucosal muscle without metastasis in 16 lymph nodes. Transvaginal specimen extraction following totally laparoscopic D2 distal gastrectomy can be safely performed in patients with SIT and has similar surgical outcomes to usual laparoscopic gastrectomy.</p

Additional file 4 of TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling

Additional file 4: Fig. S4. The effects of TRPM8 agonists on BDL-induced liver fibrosis in mice. H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in liver sections of BDL-treated mice (n = 5 per group). Image J was used to quantify positively stained areas. Scale bars, 100 μm. The results are expressed as mean ± SD

Additional file 1 of TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling

Additional file 1: Fig. S1. KEGG pathway and GO enrichment analysis

Additional file 2 of TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling

Additional file 2: Fig. S2. The effects of TRPM8 agonists on CCl4-induced liver fibrosis in mice. H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in liver sections of CCl4-treated mice (n = 5 per group). Image J was used to quantify positively stained areas. Scale bars, 100 μm. Results are expressed as mean ± SD

Additional file 3 of TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling

Additional file 3: Fig. S3. TRPM8 inhibitor reduces fibrogenesis in BDL-treated mice. A H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in liver sections of BDL-treated mice (n = 5 per group). Image J was used to quantify positively stained areas. Scale bars, 100 μm. B Serum levels of ALT and AST were measured in mice (n = 5 per group). C Expressions of α-SMA and COL1A1 were detected by immunoblotting (n = 3 per group). D Hepatic mRNAs of fibrogenic genes were measured by qRT-PCR assays in mice treated with M8-B or DMSO after BDL induction (n = 5 per group). The results are expressed as mean ± SD. *P < 0.05, **P < 0.01

Additional file 6 of TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling

Additional file 6: Table S1. Sequences of the primers used in the study. Table S2. Demographics and clinicalcharacteristics of fibrosis/cirrhosis patients with different etiologies

Additional file 5 of TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling

Additional file 5: Fig. S5. TRPM8 inhibitor attenuates ductular reaction and regulates the expression of S100A9 and HNF4α. A, B IHC staining for CK19 in liver sections of mice treated with M8-B or DMSO after CCl4 or BDL induction (n = 5 per group). Image J was used to quantify positively stained areas. Scale bars, 100 μm. C, D Expressions of TRPM8, S100A9, and HNF4α were detected by immunoblotting in the liver of mice treated with M8-B or DMSO after CCl4 or BDL induction (n = 3 per group). The results are expressed as mean ± SD. *P < 0.05, **P < 0.01