95 research outputs found

    DataSheet_1_Live birth rate following frozen-thawed blastocyst transfer is higher in high-grade day 6 blastocysts than in low-grade day 5 blastocysts.docx

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    BackgroundDay 5 (D5) blastocysts are generally given priority to transfer than day 6 (D6) blastocysts; however, which one should be prioritized to transfer when only low-grade D5 and high-grade D6 blastocysts are available?MethodsA large retrospective cohort study was carried out to evaluate the live birth rate (LBR) following D5 and D6 blastocysts in single frozen-thawed blastocyst transfer (FBT) during January 2014 and December 2018. A multivariate logistic regression was conducted to evaluate the combined impact of expansion day (D5 and D6) and blastocyst quality (high grade/low grade) on LBR, accounting for the potential confounding factors. The biopsied blastocysts from a consecutive PGT-A case series during February 2013 to December 2021 were analyzed in a supplementary study.ResultsThe LBR achieved in high-grade D6 blastocyst transfer was significantly higher than that in low-grade D5 blastocyst transfer (50.43% vs. 40.70%, aOR 1.54, 95% CI 1.05–2.26, p = 0.027). There were no significant differences in preterm birth rate, very preterm birth rate, mean live birth weight, and birth weight 4,000 g between the two cohorts. As for aneuploidy analysis in PGT, there were 54.55% of euploid blastocysts (30/55) among high-grade D6 blastocysts, significantly higher than the 41.39% of euploid blastocysts (565/1,365) among low-grade D5 blastocysts (p ConclusionsOur data suggest that D6 blastocysts with high morphology grading are preferred than D5 blastocysts with low morphology grading when selecting blastocyst transfer to shorten the time of conception.</p

    Surface modification by grafting of poly(SBMA-<i>co</i>-AEMA)-<i>g</i>-PDA coating and its application in CE

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    <div><p>In this paper, a novel copolymer consisting of sulfobetaine methacrylate (SBMA) and 2-aminoethyl methacrylate (AEMA) named as poly(SBMA-<i>co</i>-AEMA) was synthesized by conventional free-radical polymerization, the poly(SBMA-<i>co</i>-AEMA) zwitterionic copolymer was immobilized onto glass slides surface through polydopamine (PDA)-anchored coating and formed poly(SBMA-<i>co</i>-AEMA)-<i>g</i>-PDA coating. The defined copolymer was characterized by nuclear magnetic resonance hydrogen spectroscopy (<sup>1</sup>H NMR) and gel permeation chromatography. The surface morphology, thickness, and chemical component of poly(SBMA-<i>co</i>-AEMA)-<i>g</i>-PDA coating were studied by atom force microscope, ellipsometry, and X-ray photoelectron spectroscopy, respectively. The hydrophilicity and stability of these coatings were investigated by static water contact angles. And finally, the poly(SBMA-<i>co</i>-AEMA)-<i>g</i>-PDA coating was successfully applied into capillary inner surface for suppression electro-osmotic flow and protein separation by capillary electrophoresis.</p></div

    Dinuclear Aluminum Poly(phenolate) Complexes as Efficient Catalysts for Cyclic Carbonate Synthesis

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    A series of dinuclear aluminum complexes <b>1</b>–<b>4</b> stabilized by amine-bridged poly­(phenolato) ligands have been synthesized, which are highly active in catalyzing the cycloaddition of epoxides and CO<sub>2</sub>. In the presence of 0.3 mol % complex <b>3</b> and 0.9 mol % NBu<sub>4</sub>Br at 1 bar CO<sub>2</sub> pressure, terminal epoxides bearing different functional groups were converted to cyclic carbonates in 60–97% yields. Complex <b>3</b> is one of the rare examples of Al-based catalysts capable of promoting the cycloaddition at 1 bar pressure of CO<sub>2</sub>. Moreover, reactions of more challenging disubstituted epoxides also proceeded at an elevated pressure of 10 bar and afforded cyclic carbonates in 52–90% yields

    Preparation and characterization of brush-like PEGMA-<i>graft</i>-PDA coating and its application for protein separation by CE

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    <div><p>In this paper, a novel brush-like copolymer consisting of poly(ethylene glycol) methyl ether methacrylate and 2-aminoethyl methacrylate (AEMA) named as poly(PEGMA<sub>300</sub>-<i>co</i>-AEMA) was synthesized by atom transfer radical polymerization (ATRP), and then, poly(PEGMA<sub>300</sub>-<i>co</i>-AEMA) copolymer was immobilized onto material surfaces through polydopamine (PDA)-anchored coating. The defined copolymer structure was characterized by nuclear magnetic resonance hydrogen spectroscopy (<sup>1</sup>H NMR) and gel permeation chromatography (GPC). The chemical component and surface morphology of the brush-like copolymer-<i>graft</i>-PDA coating were studied by using X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM), respectively. The hydrophilicity of the brush-like copolymer-<i>graft</i>-PDA coating was investigated by using static water contact angle. The protein-resistant property of the brush-like copolymer-<i>graft</i>-PDA coating was investigated by using quartz crystal microbalance with dissipation (QCM-D), and finally the coating was applied to capillary inner surface for protein separation by capillary electrophoresis (CE).</p></div

    Dinuclear Aluminum Poly(phenolate) Complexes as Efficient Catalysts for Cyclic Carbonate Synthesis

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    A series of dinuclear aluminum complexes <b>1</b>–<b>4</b> stabilized by amine-bridged poly­(phenolato) ligands have been synthesized, which are highly active in catalyzing the cycloaddition of epoxides and CO<sub>2</sub>. In the presence of 0.3 mol % complex <b>3</b> and 0.9 mol % NBu<sub>4</sub>Br at 1 bar CO<sub>2</sub> pressure, terminal epoxides bearing different functional groups were converted to cyclic carbonates in 60–97% yields. Complex <b>3</b> is one of the rare examples of Al-based catalysts capable of promoting the cycloaddition at 1 bar pressure of CO<sub>2</sub>. Moreover, reactions of more challenging disubstituted epoxides also proceeded at an elevated pressure of 10 bar and afforded cyclic carbonates in 52–90% yields

    Morphometric analysis of ONL thickness in CeNPs- and saline- treated P23H-1 rats.

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    <p>One μl of 1 mM CeNPs (172 ng) or saline was delivered to each eye of the animals at P23 and eyes were harvested at 28 dpi; three eyes from 3 individuals were examined per treatment group. (A-D) Representative photomicrographs of H&E stained retinal sections from wildtype, Sprague Dawley (SD) or P23H-1 animals uninjected or treated with either saline or CeNPs. Similar regions were shown: 1 mm from the ONH in the inferior region. (E) shows quantification of the ONL thickness measurements. The overall ONL thickness was higher in CeNPs-treated than in saline-treated animals although the increases were not statistically significant across many of the regions. INL = inner nuclear layer, ONL = outer nuclear layer, I/OS = rod inner/outer segment, ONH = optic nerve head. *P<0.05.</p

    Defining the Catalytic Activity of Nanoceria in the P23H-1 Rat, a Photoreceptor Degeneration Model

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    <div><p>Purpose</p><p>Inorganic catalytic nanoceria or cerium oxide nanoparticles (CeNPs) are bona fide antioxidants that possess regenerative radical scavenging activities <i>in vitro</i>. Previously, we demonstrated that CeNPs had neuroprotective and anti-angiogenic properties in rodent retinal degeneration and neovascularization models. However, the cellular mechanisms and duration of the catalytic activity of CeNPs in preventing photoreceptor cell loss are still unknown. In this study, we sought to answer these questions using the P23H-1 rat, an autosomal dominant retinitis pigmentosa (adRP) model.</p><p>Methods</p><p>A single dose of either saline or CeNPs was delivered intravitreally into the eyes of P23H-1 rats at 2–3 weeks of age. Retinal functions were examined at 3 to 7 weeks post injection. We quantified retinal proteins by Western blot analyses and counted the number of apoptotic (TUNEL+) profiles in the outer nuclear layer (ONL) of retinal sections. We measured free 8-isoprostanes to quantify lipid peroxidation in retinal tissues.</p><p>Results</p><p>We observed increased rod and cone cell functions up to three weeks post injection. Apoptotic cells were reduced by 46%, 56%, 21%, and 24% at 3, 7, 14, 21 days, respectively, after CeNPs injection compared to saline. Additionally, reduction of lipid peroxidation in the retinas of CeNPs-treated vs saline-treated animals was detected 14 days post injection.</p><p>Conclusions</p><p>We validated that CeNPs were effective in delaying loss of photoreceptor cell function in an adRP rat model. This represents the fourth rodent retinal disease model that shows delay in disease progression after a single application of CeNPs. We further demonstrated that CeNPs slowed the rate of photoreceptor cell death. We deduced that the catalytic activity of CeNPs <i>in vivo</i> in this rat model to be undiminished for at least 7 days and then declined over the next 14 days after CeNPs administration.</p></div

    A Comparative Study on Dinuclear and Mononuclear Aluminum Methyl Complexes Bearing Piperidyl–Phenolato Ligands in ROP of Epoxides

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    Dinuclear aluminum methyl complexes stabilized by piperidyl–phenolato ligands were prepared and characterized. The ring-opening polymerizations of cyclohexene oxide (CHO) and propylene oxide (PO) initiated by dinuclear complexes and mononuclear analogues were investigated and compared. Enhanced activity of dinuclear complexes compared to that of mononuclear analogues in both the ring-opening polymerization of CHO and PO proves the synergistic interaction of two Al centers in the former. End-group analysis of oligomers by MALDI-TOF mass spectrometry confirms the role of methyl groups as initiating groups. A bimetallic mechanism is proposed, in which the cooperation of two Al centers are involved in polymerization processes

    Effect of hypoxia preconditioned CPCs in treating MI.

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    <p>CPCs were cultured under either normoxia (Nx) or hypoxia (Hx) for six hours with or without AMD3100 (CXCR4-selective antagonist, 5 µg/mL) and then intramyocardially injected into mice after surgical MI. Mice in the control group were administered DMEM. (A) Echocardiographic measurements were performed seven days after CPC transplantation. Representative M-mode images at the level of papillary muscles were recorded. (B) Representative Masson’s trichrome staining of transverse heart sections seven days after coronary ligation and CPC administration. (C) Representative phase micrographs show TUNEL-positive apoptotic cells in bordering myocardium adjacent to the infarct zone seven days after coronary ligation (×200 magnification). (D) Quantitative analysis of infarct size. Values are expressed as mean ± SD. n = 5. *<i>P</i><0.05 vs. control group, **<i>P</i><0.01 vs. control group, # <i>P</i><0.05 vs. normoxia (Nx) & hypoxia (Hx) + AMD group. (E) Quantitative analysis of apoptotic cells in bordering myocardium. Values are expressed as mean ± SD. n = 5. *<i>P</i><0.01 vs. control group, <sup># </sup><i>P</i><0.01 vs. normoxia (Nx) & hypoxia (Hx) + AMD group. (F) Representative TUNEL+ apoptotic engrafted CPCs in heart tissues which were collected 72 h after cell transplantation (×200 magnification). (G) The percentage of apoptotic engrafted CPCs. The number of apoptotic engrafted CPCs was assessed by double positive of GFP+/TUNEL+ (yellow). Values are expressed as mean ± SD. n = 5. *<i>P</i><0.01 vs. normoxia (Nx) group & hypoxia (Hx) + AMD group.</p
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