Light-Powered Tumbler Movement of Graphene Oxide/Polymer Nanocomposites

Photoresponsive lamina and flexible graphene oxide/polymer nanocomposite films were fabricated using a simple solution casting method. Fast, stable, and reversible photomechanical behavior of the nanocomposite films upon irradiation with visible light was observed based on the photothermal effect of graphene oxide and the shape memory effect of the polymer matrix. According to the principle of equilibrium apparatus, light-powered tumbler movement was achieved in these films by imitating the structure of a wobbly man. Although photodriven contraction, expansion, bending, twisting, oscillation, and cilia movement have been realized in photomechanical materials, novel forms of complicated motion are still a bottleneck problem limiting their practical applications. This work would have a significant impact on photomechanical materials in device applications for advanced functions

Light-Powered Tumbler Movement of Graphene Oxide/Polymer Nanocomposites

Photoresponsive lamina and flexible graphene oxide/polymer nanocomposite films were fabricated using a simple solution casting method. Fast, stable, and reversible photomechanical behavior of the nanocomposite films upon irradiation with visible light was observed based on the photothermal effect of graphene oxide and the shape memory effect of the polymer matrix. According to the principle of equilibrium apparatus, light-powered tumbler movement was achieved in these films by imitating the structure of a wobbly man. Although photodriven contraction, expansion, bending, twisting, oscillation, and cilia movement have been realized in photomechanical materials, novel forms of complicated motion are still a bottleneck problem limiting their practical applications. This work would have a significant impact on photomechanical materials in device applications for advanced functions

Light-Powered Tumbler Movement of Graphene Oxide/Polymer Nanocomposites

Photoresponsive lamina and flexible graphene oxide/polymer nanocomposite films were fabricated using a simple solution casting method. Fast, stable, and reversible photomechanical behavior of the nanocomposite films upon irradiation with visible light was observed based on the photothermal effect of graphene oxide and the shape memory effect of the polymer matrix. According to the principle of equilibrium apparatus, light-powered tumbler movement was achieved in these films by imitating the structure of a wobbly man. Although photodriven contraction, expansion, bending, twisting, oscillation, and cilia movement have been realized in photomechanical materials, novel forms of complicated motion are still a bottleneck problem limiting their practical applications. This work would have a significant impact on photomechanical materials in device applications for advanced functions

Light-Powered Tumbler Movement of Graphene Oxide/Polymer Nanocomposites

Photoresponsive lamina and flexible graphene oxide/polymer nanocomposite films were fabricated using a simple solution casting method. Fast, stable, and reversible photomechanical behavior of the nanocomposite films upon irradiation with visible light was observed based on the photothermal effect of graphene oxide and the shape memory effect of the polymer matrix. According to the principle of equilibrium apparatus, light-powered tumbler movement was achieved in these films by imitating the structure of a wobbly man. Although photodriven contraction, expansion, bending, twisting, oscillation, and cilia movement have been realized in photomechanical materials, novel forms of complicated motion are still a bottleneck problem limiting their practical applications. This work would have a significant impact on photomechanical materials in device applications for advanced functions

Characterization and hyperosmotic treatment of the mouse conjunctival epithelium primary cells.

<p>The confirmation of the origin of primary epithelial cells was done by assessing the levels of cytokeratin 4(K4) and cytokeratin 12(K12) by RT-qPCR (Fig 3A). Under hyperosmotic conditions (350 mOsm and 400 mOsm) for 24h, significant increase in the mRNA expression of proinflammatory cytokine markers, TNF-α and IL-1β, was noted (Fig 3B).</p

Co-immunoprecipitation (CoIP) of AQP5 and MUC5AC.

<p>(2A) The detection of MUC5AC in Ab-Ag complex by immunoprecipitation of AQP5 from rabbit conjunctival tissue protein lysate with AQP5 antibody bound to Dynabead^® Magnetic beads coupled Protein A. Input: The normal rabbit conjunctival tissue protein lysate (Positive control for MUC5AC); Co-IP: MUC5AC protein signal detected in the Co-IP Ab-Ag complex by AQP5 antibody; Negative controls: Beads+AQP5 (magnetic beads incubated with only the AQP5 antibody); Beads + rabbit IgG (magnetic beads incubated with only the IgG antibody); Beads only (magnetic beads alone incubated in the reaction buffer). (2B) The CoIP of AQP5 by immunoprecipitation of MUC5AC from rabbit conjunctival tissue protein lysate with MUC5AC antibody bound to Dynabead^® Magnetic beads coupled Protein A. Input: The normal rabbit conjunctival tissue protein lysate (Positive control for AQP5); Co-IP: AQP5 protein signal detected in the Co-IP Ab-Ag complex by MUC5AC antibody; Negative controls: Beads+ MUC5AC: Beads + rabbit IgG; Beads only.</p

Increase in conjunctival AQP5 and MUC5AC expression in rabbits after surgery.

<p>In comparison to before excision (BE), the AQP5 protein increased significantly at 1, and 2 months after excision (AE), and then decreased slightly at the end of 3 months AE. The MUC5AC protein expression followed similar pattern of upregulation, demonstrating a synchronous relationship with AQP5. The protein (immunoblot) signals of AQP5 and MUC5AC were presented relative to the internal control “Gapdh” signal.</p

Table_1_A novel immune-related gene signature predicts the prognosis of hepatocellular carcinoma.xls

Immune-related genes play a key role in regulating the cancer immune microenvironment, influencing the overall survival of patients with hepatocellular carcinoma (HCC). Along with the rapid development of immunotherapy, identifying immune-related genes with prognostic value in HCC has attracted increasing attention. Here, we aimed to develop a prognostic signature based on immune-related genes. By investigating the transcriptome landscape of 374 HCC and 160 non-HCC samples in silico, a total of 2251 differentially expressed genes were identified. Among which, 183 differentially expressed immune-related genes were subjected to a univariate Cox proportional hazard model to screen for genes with possible prognostic significance. A 10-gene prognostic signature, including HLA-G, S100A9, S100A10, DCK, CCL14, NRAS, EPO, IL1RN, GHR and RHOA, was generated employing a multivariate Cox proportional hazard model. Kaplan–Meier and Receiver Operator Characteristic (ROC) curves were used to evaluate the prognostic utility of the 10-gene signature. Moreover, the underlying mechanisms of these genes were analyzed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. According to the Tumor Immune Estimation Resource (TIMER) database, our prognostic signature was significantly associated with tumor-infiltrating B cells, CD4 T cells, dendritic cells, macrophages and neutrophils. Our study provides a novel prognostic signature based on immune-related genes associated with clinical outco mes of HCC.</p

Characterization of M35 family genes from nine fungi we used.

<p>Characterization of M35 family genes from nine fungi we used.</p

Hybrid Inorganic–Organic Polyrotaxane, Pseudorotaxane, and Sandwich

Inorganic copper­(I)/silver­(I) halide/pseudohalide components are used to thread classical organic tetracationic macrocycles, cyclobis­(paraquat-<i>p</i>-phenylene) and cyclobis­(paraquat-4,4′-biphenylene), to construct crystalline inorganic–organic adducts, featuring an unprecedented hybrid polyrotaxane and several unusual hybrid pseudorotaxanes and sandwiches