Effect of CCN3 overexpression on CCN1 and CCN2 expression.

<p>The mRNA levels of CCN1 (A) and CCN2 (B) in tissues of the left common carotid artery were analyzed by qRT-PCR. *p<0.05 vs. PBS or Ad-GFP denotes a statistically significant difference.</p

Expression of CCN3 in atherosclerosis.

<p>The mRNA level (A) and protein level (B) of CCN3 in the left common carotid artery of mice were evaluated by qRT-PCR and Western blot analysis, respectively. 1 represents control mice (wild-type C57BL; 6 mice); 2 represents 25-week-old ApoE^−/− mice fed a high-fat diet. GAPDH was used as a control. *p<0.05, <i>N</i> = 30 per group.</p

Effect of overexpression of CCN3 on atherosclerosis.

<p>CCN3 mRNA (A) and protein (B) levels in different treatment groups of mice were analyzed by qRT-PCR and Western blot, respectively. PBS, mice injected with PBS as a control; Ad-GFP, mice injected with recombinant adenovirus expressing GFP as a non-specific control; Ad-CCN3, mice injected with recombinant adenovirus expressing CCN3. Serum levels of LDL cholesterol (C), HDL cholesterol (D), total cholesterol (E), and triglycerides (F) in the three groups of mice are shown. Cross-section histological analyses of plaques stained by HE (G) are shown. Quantitative analysis of plaque area (H), fibrous cap (I), cap-to-core ratio (J), and intima-media thickness (K) in the three groups of mice are also shown. *p<0.05 vs. PBS or Ad-GFP denotes a statistically significant difference.</p

Effect of proinflammatory cytokines on CCN3 expression.

<p>HAECs (A) and HUVECs (B) were treated with TNF-α (10 ng/ml) or IL-1β (4 ng/ml) for 24 h. Total RNA and cell protein were harvested for qRT-PCR or Western blot analysis, respectively. mRNA expression was normalized to GAPDH (<i>N</i> = 6, *p<0.05 and **p<0.01). Protein loading was normalized to equal amounts of cells.</p

Effect of CCN3 overexpression on gene expression of adhesion molecules.

<p>qRT-PCR was used to analyze the mRNA levels of VCAM-1 (A) and ICAM-1 (B) in tissues of the left common carotid artery. *p<0.05 or **p<0.01 vs. PBS or Ad-GFP denotes a statistically significant difference.</p

Pyrénées et océan : organe des stations thermales et des bains de mer desservant tout le littoral et la chaîne des Pyrénées, chronique mondaine du baigneur et du touriste... ["puis" journal des intérêts économiques des Pyrénées et du Sud-Ouest : organe officiel de la Fédération des syndicats d'initiative du Sud-Ouest]

31 mars 19071907/03/31 (N86).Appartient à l’ensemble documentaire : Aquit1Appartient à l’ensemble documentaire : MidiPyren

Experimental Study on Effect of Simulated Microgravity on Structural Chromosome Instability of Human Peripheral Blood Lymphocytes

<div><p>Experimental study was made by keeping human peripheral blood lymphocytes under simulated microgravity in a Rotary Cell Culture System bioreactor to investigate the changes that occur in the number of chromosomes, the expression rate of chromosome fragile site, and the expressions of DNA replication- and repair-related genes. Experimental results indicate simulated microgravity has no effect on the numerical chromosome instability of human peripheral blood lymphocytes, but it enhances the structural chromosome instability of human peripheral blood lymphocytes through the inhibition of DNA replication and the reduction of DNA repair. So, the mechanism of chromosome fragile site induced by simulated microgravity can be explained using the changes that occur in the chromosome structure of human peripheral blood lymphocytes, the DNA replication and repair under the effect of simulated microgravity.</p></div

Laccase production by <i>Phomopsis liquidambari</i> B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

<div><p>Food waste is the most difficult waste to manage because of the high treatment costs and the risk of environmental contamination. Wheat straw burning has become an increasingly discussed topic in recent years because of the air pollution it causes. However, food waste and wheat straw contain high amounts of nutrient elements; efficient utilization of these wastes can improve the environment by lessening airborne pollutant emission and the amount of waste that must be landfilled. It is well known that use of low-cost and abundant waste materials in microbial fermentations can reduce product costs. We aimed to use these resources while improving laccase production by the endophytic fungus <i>Phomopsis liquidambari</i> B3. We cultured <i>P. liquidambari</i> B3 in medium containing food waste and wheat straw as the main nitrogen and carbon sources, respectively. We optimized the fermentation conditions by response surface methodology (RSM), using a Box–Behnken design for RSM I and a central composite design for RSM II. Optimization resulted in an 11.07-fold (1.98-fold RSM I; 5.59-fold RSM II) increase in laccase yield compared with that before optimization. The model was validated by mathematical evaluations and by comparisons between predicted and experimental values. Under optimized conditions, 53.76% of lignin in wheat straw was degraded. By optimizing fermentation conditions and using multiple bioresources, laccase production by this fungus was increased. These results provide the foundation for future research and for scaled-up laccase production. </p><p></p><p>Implications:</p><p>Food waste and wheat straw are waste products, but they could be bioresources if they were managed properly. In this work, we innovatively used a mixture of food waste and wheat straw as a substrate for the novel strain <i>Phomopsis liquidambari</i> B3 to produce laccase. This process, which provided the foundation for the subsequent research and for scaled-up laccase production, offers solutions for both rural and urban pollution problems: that is, it reduces the amount of waste material that needs to be disposed of by burning or dumping, and it also produces a valuable product.</p><p></p><p></p></div

Additional file 12: Table S8. of Dynamic transcriptome and DNA methylome analyses on longissimus dorsi to identify genes underlying intramuscular fat content in pigs

The differentially methylated cytosines (DMCs) and corresponding genes in 120 and 240 days old Laiwu pigs. The DMCs were produced by sequencing depth ≥ 4×, and FDR ≤ 0.05. (XLSX 6864 kb

Number of chromosome fragile sites in 100 cells of 3 independent samples.

<p>The PBL cells were cultured in folate-free M199 and 0.4 µM aphidicolin added medium. 100 mitotic images of were collected from each sample, and both the number of cells observed and the number of chromosomes with fragile sites were counted. The chromosome fragile sites are determined according to nonstaining chromosome, chromatid gaps or chromosome breakpoints. The number of chromosome fragile sites is much higher for the cells after the cells being kept under simulated microgravity for 72 hours than that of the untreated control group for the three independent samples. *0.01<<i>P</i><0.05 and **<i>P</i><0.01 (-test as compared to control group).</p