Virulence of ASFV BA71 strain.

<p>(A) Rectal temperatures were daily recorded after infection of pigs with 10² or 10⁴ HAU50 of BA71. (B) Survival of pigs after infection with 10² or 10⁴ HAU50 of BA71.</p

Differences between BA71 and BA71V: Large deletions/insertions.

<p>The positions in the genome of BA71 and BA71V of the sequences affected are indicated along with the size of the region not present in either of the genomes, in brackets. The genes affected by theses differences are also indicated.</p><p>Differences between BA71 and BA71V: Large deletions/insertions.</p

Differences between BA71 and BA71V: Variations in the number or in the sequence of tandem repeats.

<p>The positions in the genome of BA71 and BA71V of the sequences affected are indicated along with the number of repeats involved in the variation, and the identity of the genomic feature affected by the change. The size of the region not present in either of the genomes is indicated in brackets. In the case of difference 33, the highly variable central region located in ORF B602L, 4 of the 29 repeats of 12 nucleotides that compose this region have different sequence but there is no variation in the number of nucleotides.</p><p>Differences between BA71 and BA71V: Variations in the number or in the sequence of tandem repeats.</p

Genomic sequences used in this study.

<p>The table shows general information about the genomic sequences of the ASFV strains used in this study. Left and right indicates the size of the left and right genome variable regions. The left variable region includes the nucleotides from the 5’- end of the genome until gene A224L, whereas the right variable region includes the nucleotides from the 3’- end of the genome until gene DP238L.</p><p>Genomic sequences used in this study.</p

Differences between BA71 and BA71V: Point mutations.

<p>The positions of the point mutations in both genomes are indicated along with the nature of the nucleotides at each position, in brackets. The ORF implicated, its known function and the mutation produced in the protein sequence are also indicated. The asterisk indicates that a comparison with the sequences of other European isolates shows that the mutation has occurred in the virulent BA71 isolate.</p><p>Differences between BA71 and BA71V: Point mutations.</p

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 17.

<p>The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 17 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The ORFs are members of MGF 505.</p

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 49.

<p>The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 49 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as shown in the figure. The dash at the beginning of the names of some ORFs indicates where the prefix BA71- has been removed to avoid clutter.</p

Differences between BA71 and BA71V: changes in polynucleotide sequences.

<p>The position of the differences in each genome is indicated along with the size of the change, in brackets. The nature of the polynucleotide and the ORF affected, if any, are also indicated.</p><p>Differences between BA71 and BA71V: changes in polynucleotide sequences.</p

5’- and 3’- ends of the ASFV BA71 and BA71V genomes.

<p>The figure shows dot plot comparisons of the 5’- (A) and 3’-end (B) variable regions. For each of the four large deletions observed between the genomes, the deleted region is indicated by a light blue box in the corresponding axis and the position where the deletion occurred is indicated by an arrow in the opposite axis. ORFs are indicated by arrows, and the different groups of ORFs are identified by colors: MGF 360, violet; MGF 110, yellow; MGF 300, green; MGF 505, red; MGF 100, rose; p22 family, gray, other ORFs, black.</p

Clinical characteristics of osteosarcoma patients (<i>N</i> = 102).

<p>Clinical characteristics of osteosarcoma patients (<i>N</i> = 102).</p