6 research outputs found

    Correlation between DC maIL-15 and A3G mRNA or protein in CD4<sup>+</sup> central and effector T or B memory cells.

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    <p>Correlation between maIL-15 on DC and A3G mRNA in PBMC (A<b>–</b>C), intracellular A3G protein in CD4<sup>+</sup>CD95<sup>+</sup>CCR7<sup>+</sup> central memory cells (D<b>–</b>F), CD4<sup>+</sup>CD95<sup>+</sup>CCR7<sup>−</sup> effector memory cells (G,I), CD20<sup>+</sup> B cells (J<b>–</b>L) or CD20<sup>+</sup>CD27<sup>+</sup> memory B cells (M<b>–</b>O) in the combined groups (1<b>–</b>5), group 1 or group 3 macaques, respectively. IL-15 and A3G were assayed after the last immunization and before the animals were challenged with SHIV SF162.P4. In all figures the 2 uninfected macaques in group 3 are indicated by a solid circle.</p

    AID and A3G expression in CD20<sup>+</sup> B cells pre- and post-2<sup>nd</sup> immunization and their correlation.

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    <p>Comparative investigation of (A) AID and (B) A3G expression in CD20<sup>+</sup> B cells before and after the 2<sup>nd</sup> immunization in the 4 groups of immunized macaques; group 5 unimmunized controls remained unchanged (data not presented). Correlation between A3G and AID expression in CD20<sup>+</sup> B cells in the 5 groups after 2<sup>nd</sup> immunization is presented in (C). Representative flow cytometry of AID and A3G in pre- and post 2<sup>nd</sup> immunization is shown in (D). * p<0.05 and ** p<0.01. In all figures the 2 uninfected macaques in group 3 are indicated by a solid circle.</p

    Membrane-associated (ma) IL-15 of DC and CD40L expression of CD4<sup>+</sup> T cells in 5 groups of macaques.

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    <p>(A) Membrane-associated (ma) IL-15 of DC and (B) CD40L expression on CD4<sup>+</sup> T cells in 5 groups of macaques pre- and post-4<sup>th</sup> immunization, presented as % mean (±sem). The significance between pre- and post-immunization was analysed by paired “t” test and differences between the 5 groups after immunization was analysed by ANOVA.</p

    A3G in CD20<sup>+</sup> and CD27<sup>+</sup> memory B cells pre- and post-immunization in the 4 groups.

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    <p>Expression of A3G in (A) CD20<sup>+</sup> B cells and (B) CD20<sup>+</sup>CD27<sup>+</sup> memory B cells in 5 groups of macaques before and after the 4<sup>th</sup> immunization assayed by flow cytometry with MAb to A3G, CD20 and CD27 and (C) representative illustration; (n = 8 per group, except gp4 n = 6). * p<0.05. In all figures the 2 uninfected macaques in group 3 are indicated by a solid circle.</p

    Correlation between the viral load and A3G mRNA, protein in B cells or AID.

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    <p>Correlation between PVL or CVL and A3G mRNA in PBMC (A,B), AID (C, D), A3G proteins in CD20<sup>+</sup> B cells (E,F) and A3G in CD4<sup>+</sup>CD95<sup>+</sup>CCR7<sup>−</sup> effector memory T cells (G,H). AID and A3G were assayed after the 4<sup>th</sup> immunization, whereas CVL was calculated as the “area under the curve”. The two protected macaques are shown by open circles. Pearson's correlation coefficient was used for statistical analysis. In all figures the 2 uninfected macaques in group 3 are indicated by a solid circle.</p

    Correlation between A3G memory B and CD4<sup>+</sup> T cells, and A3G mRNA with HLA or neutralizing antibodies.

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    <p>Correlation between A3G in CD20<sup>+</sup>CD27<sup>+</sup> memory B cells and CD4<sup>+</sup>CD95<sup>+</sup>CCR7<sup>−</sup> effector memory T cells (A) in all 5 groups, (B) in group 1 (without SHIV antigens) and (C) in group 3 macaques. Correlation between A3G mRNA in PBMC and serum anti-HLA class I antibodies (D<b>–</b>F), anti-HLA class II antibodies (MFI) (G<b>–</b>I) assayed by the Luminex HLA antibody method and neutralizing activity (J<b>–</b>L) determined by using a TZM-b1 assay in the corresponding groups. In all figures the 2 uninfected macaques in group 3 are indicated by a solid circle.</p
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