426 research outputs found
sj-sav-1-hpq-10.1177_13591053211062347 – Supplemental material for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life
Supplemental material, sj-sav-1-hpq-10.1177_13591053211062347 for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life by Shuai Jin, Lei Zheng, Jie Wen and Miao Miao in Journal of Health Psychology</p
sj-docx-9-hpq-10.1177_13591053211062347 – Supplemental material for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life
Supplemental material, sj-docx-9-hpq-10.1177_13591053211062347 for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life by Shuai Jin, Lei Zheng, Jie Wen and Miao Miao in Journal of Health Psychology</p
Additional file 1 of Cell competition between wild-type and JAK2V617F mutant cells prevents disease relapse after stem cell transplantation in a murine model of myeloproliferative neoplasm
Additional file 1. Materials and methods
Additional file 1 of A prediction model for predicting the risk of acute respiratory distress syndrome in sepsis patients: a retrospective cohort study
Additional file 1: Table S1. The difference analysis between the training set and testing se
Direct Screening for Cytometric Bead Assays for Adenosine Triphosphate
Cytometric
bead assays have caught much attention because of their many exceptional
advantages. Unfortunately, the immobilization of existing molecular
recognition elements including monoclonal antibodies and aptamers
onto solid particles may lead to the functional failure of the molecular
recognition elements since they are generally obtained in free state.
Herein we develop a powerful screening approach for direct and rapid
discovery of aptamer based cytometric bead assays (AB-CBAs) by individually
measuring the functional activity of every aptamer particles in a
library and sorting them at rates of up to 108 particles
per hour. The strategy is based on the transformation of molecular
libraries into pools of monoclonal aptamer particles so that one individual
particle displays ∼105 copies of an identical aptamer
sequence. Our library design incorporates a two-color fluorescent
reporter system in which changes in aptamer structure generate an
optical readout, such that we can use fluorescence-activated cell
sorting to rapidly and selectively separate the individual aptamer
particles that exhibit large fluorescent signal change upon target
binding. For demonstration, we isolated AB-CBA aptamer particles with
high signaling performance for ATP after just 3 rounds of screening.
We believe that the rapid and direct screening features of this strategy
make it an excellent platform for generating AB-CBAs for for a wide
range of important analytes
sj-spv-6-hpq-10.1177_13591053211062347 – Supplemental material for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life
Supplemental material, sj-spv-6-hpq-10.1177_13591053211062347 for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life by Shuai Jin, Lei Zheng, Jie Wen and Miao Miao in Journal of Health Psychology</p
sj-rar-5-hpq-10.1177_13591053211062347 – Supplemental material for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life
Supplemental material, sj-rar-5-hpq-10.1177_13591053211062347 for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life by Shuai Jin, Lei Zheng, Jie Wen and Miao Miao in Journal of Health Psychology</p
sj-rar-7-hpq-10.1177_13591053211062347 – Supplemental material for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life
Supplemental material, sj-rar-7-hpq-10.1177_13591053211062347 for The relationship between active coping and hope during the COVID-19 pandemic: The mediating role of meaning in life by Shuai Jin, Lei Zheng, Jie Wen and Miao Miao in Journal of Health Psychology</p
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