Supplementary_Table_2._Attentional_bias_ANOVA_results – Supplemental material for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation

Supplemental material, Supplementary_Table_2._Attentional_bias_ANOVA_results for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation by Daniel E Schoth, Laura Parry and Christina Liossi in Journal of Health Psychology</p

Supplementary_Table_1._Stimuli – Supplemental material for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation

Supplemental material, Supplementary_Table_1._Stimuli for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation by Daniel E Schoth, Laura Parry and Christina Liossi in Journal of Health Psychology</p

Supplementary_Tables_3a_-_3f._Correlations – Supplemental material for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation

Supplemental material, Supplementary_Tables_3a_-_3f._Correlations for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation by Daniel E Schoth, Laura Parry and Christina Liossi in Journal of Health Psychology</p

Supplementary_Figure_1 – Supplemental material for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation

Supplemental material, Supplementary_Figure_1 for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation by Daniel E Schoth, Laura Parry and Christina Liossi in Journal of Health Psychology</p

Supplementary_Questionnaire_Information_and_Data – Supplemental material for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation

Supplemental material, Supplementary_Questionnaire_Information_and_Data for Combined cognitive biases for pain and disability information in individuals with chronic headache: A preliminary investigation by Daniel E Schoth, Laura Parry and Christina Liossi in Journal of Health Psychology</p

Additional file 1: Figure S1. of Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation

Expression of (a) Rxfp1, (b) Il1β, (c) VegfA total, (d) Vegfr2, (e) Esr1, (f) Esr2, (g) Mmp14 and (h) Timp3 in the uterus of Rln +/+ and Rln -/- mice on day 6 of pregnancy (Study 3, n = 6–7). Horizontal bars indicate mean values. (PDF 35 kb

Additional file 2: Figure S2. of Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation

Expression of (a) 18 s, (b) PpiA, (c) Sdha and (d) Tbp in the uterus of Rln +/+ mice on days 1 to 4 of pregnancy (n = 6–8). Horizontal bars indicate mean values. Groups that do not share a letter are significantly different from one another (p < 0.05). (PDF 37 kb

Additional file 3: Table S1. of Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation

Primer and probe sequences for the quantitative amplification of murine genes. Table S2. Mean CT values and the range of CT values for each gene analyzed by qPCR in the uterus of wildtype (Rln +/+ ) and relaxin deficient (Rln -/- ) mice (n = 6–8). Table S3. Primer sequences for the RT-PCR amplification of murine genes. (DOC 111 kb

Ocular Phenotype of Relaxin Gene Knockout (Rln^-/-) Mice

Purpose: To test if relaxin deficiency affects ocular structure and function we investigated expression of relaxin (Rln) and RXFP receptors (Rxfp1, Rxfp2), and compared ocular phenotypes in relaxin gene knockout (Rln−/−) and wild type (Rln+/+) mice. Materials and Methods: Rln, Rxfp1 and Rxfp2 mRNA expression was detected in ocular tissues of Rln+/+ mice using RT-PCR. The eyes of 11 Rln−/− and 5 Rln+/+ male mice were investigated. Corneal and retinal thickness was assessed using optical coherence tomography. Intraocular pressure was measured using a rebound tonometer. Retinal, choroidal and sclera morphology and thickness were evaluated histologically. Eyes were collected and fixed for immunofluorescence staining or used for RNA extraction to evaluate mRNA expression using real-time PCR. Results: Rln mRNA was expressed only in the retina, whereas Rxfp1 transcripts were detected in the retina, cornea and sclera/choroid. Rxfp2 was only present in the cornea. None of these genes were expressed in the lacrimal gland, eyelid or lens. Intraocular pressure was higher and central cornea of Rln−/− mice was significantly thicker and had significantly larger endothelial cells and a lower endothelial cell density than Rln+/+ mice. Immunohistochemistry demonstrated no significant difference in AQP3 and AQP5 staining in the cornea or other regions between wildtype and Rln−/− mice. mRNA expression of Aqp4 was significantly higher in Rln−/− than in Rln+/+ corneas, whereas Col1a2, Mmp9, Timp1 and Timp2 were significantly decreased. Expression of Aqp1, Aqp4, Aqp5, Vim and Tjp1 was significantly decreased in Rln−/− compared to Rln+/+ uvea. No significant differences in these genes were detected in the retina. Retinal, choroidal and scleral thicknesses were not different and morphology appeared normal. Conclusion: The findings indicate that loss of Rln affects expression of several genes in the uvea and cornea and results in thicker corneas with altered endothelial cells. Many of the gene changes suggest alterations in extracellular matrix and fluid transport between cells.</p