Ethnobotanical use, phytochemical study, and antioxidant activity of Globularia alypum

This work aimed to highlight the biological effects of Globularia alypum L., a medicinal plant widely used in phytotherapy.Globularia alypum L. is a plant used in traditional medicine in Morocco, the crude extracts prepared from the leaves were obtained using maceration in methanol, ethyl acetate, butanol, and water. The content of total polyphenols was determined using the Folin-Ciocalteu reagent, the first range was for the ethyl acetate extract, followed by butanol, methanol, and aqueous extracts, respectively. The flavonoids amount was determined using the aluminum chloride colorimetric method, the methanolic extract presented the highest content of flavonoids, followed by the one of butanol, then the ethyl acetate and aqueous extracts. The antioxidant activity was carried out using the anti-free radical method based on 2,2-diphenyl-1-picrylhydrazyle (DPPH), the IC50 values were estimated for methanolic, ethyl acetate, butanol and aqueous extracts while that of BHT and ascorbic acid. The high phenolic and flavonoid amounts as well as the antioxidant potential of the extracts indicated that the G. alypum could be exploited as a source of natural antioxidants

An ethnobotanical investigation of medicinal and aromatic plants used in Al Hoceima National Park

In the northern region of Morocco, specifically in the Province of Al Hoceima, the establishment of the Al Hoceima National Park (ANP) serves as a designated protected area. The primary purpose of this park is to promote the conservation, protection, and sustainable development of the diverse natural resources present. The ANP boasts a significant variety of flora, particularly medicinal and aromatic plants (AMP). This research aimed to identify and highlight the AMP the local rural population utilizes. Conducted between August 2020 and March 2021, an ethnobotanical survey engaged herbalists, traditional healers, and rural residents through structured questionnaires. These surveys elicited specific information about the informants and details regarding the plants in use. This survey revealed the identification of 47 taxa based on vernacular names, which were subsequently matched with their scientific names according to available literature. These taxa are distributed among 29 botanical families, with notable prevalence in Lamiaceae, comprising 8 species, followed by Asteraceae, Fabaceae, Rosaceae, and Solanaceae, each with 3 species. The study further indicates that the most utilized parts of these plants are the foliage and leafy stems, with decoctions being the predominant method of preparation for remedies. The investigation into the diseases treated revealed that digestive infections rank highest, followed by respiratory infections. Sociodemographic characteristics analysis indicated a slightly higher usage of medicinal plants by women than men. The insights gleaned from this research offer valuable information for the studied region and contribute to the broader understanding of the national medicinal flora

Phytochemical Study and Antioxidant Potential of Three Leaf Extracts of Cannabis sativa L from the Al-Hoceima Region, Morocco

Within Morocco's efforts to increase the value of cannabis, we investigated the 'Beldeya' cannabis sativa L plant, which is produced in the Ketama area of northern Morocco. In this investigation, we performed phytochemical screening and quantitative analyses of ethanol, chloroform, and hexane extracts from cannabis leaves. Additionally, we explored the antioxidant potential using two extraction methods: Maceration and Soxhlet extraction. According to the results of the phytochemical screening, most metabolites were present in the extracts, except for alkaloids and anthraquinones which were not detected in any of the extracts. We employed standard methods for the quantitative measurement of total phenol (TPC) and flavonoid content (TFC), and the results showed that the TPC and TFC were abundantly present in the hexane extract using both maceration and Soxhlet extraction methods. Antioxidant potential was assessed through the Assay for reducing 2,2-diphenyl-1-picrylhydrazyl(DPPH). The findings demonstrate that the ethanolic extract shown greater activity for both extraction techniques, displaying an IC50 value of 222.96 μg/ml for Soxhlet extraction and 256.21 μg/ml for the maceration method

Qualitative and Quantitative Study of the Bioactive Compounds in

The qualitative and quantitative analysis aims to identify secondary metabolites, including phenolic compounds, alkaloids, terpenes, and other substances commonly referred to as phytochemicals. Qualitative phytochemical screening relies on color and precipitation tests that indicate the presence or absence of a metabolite, while quantitative analysis accounts for the quantity or concentration of the bioactive compounds contained within the extracts. During the quantitative determination of the total content of phenolics and flavonoids present in the extracts, we used the conventional soxhlet extraction (SE) method with solvents of different polarities. The purpose of this study is to ascertain the impact of solvents with varying polarity on both the extraction yield and phytochemical composition of A. unedo leaves. The findings indicate that yield and polyphenol content vary from one extract to another. Thus, the ethanol extract (EEAU) showed the highest percentage extraction yield (15.23%) and the highest total phenolic content (TPC) with 122.82 ± 5.42 mg GAE/gext. In contrast, the dichloromethane extract (EDAU) had the lowest extraction yield (1.76%), while the methanol extract (EMAU) showed the highest total flavonoid content (TFC) with 45.8 ± 1.3 mg QE/gext. Generally, these data provide information into the phytochemical content of various extracts of the plant growing in the Ait Ammart region. These extracts contain several bioactive substances that will be useful in the formulation of pharmaceutical products for a variety of diseases and may have applications in the food industry

Phytochemical, antioxidant, and anti-inflammatory properties of

Tetraclinis articulata (Vahl) Masters (TA), a member of the Cupressaceae family, is frequently used in traditional medicine to treat various health problems in Al Hoceima Province. Our study aimed to determine the content of polyphenols and flavonoids, and to evaluate the biological antioxidant activities using DPPH and FRAP tests. At the same time, we assessed the anti-inflammatory activity of various extracts of TA from Al Hoceima, using the BSA method for each of the extracts studied. The results reveal that ethyl acetate extracts (28.11 mg EAG/g ES), followed by ethanolic extracts (22.42 mg EAG/g ES), show higher quantities than the other extracts. Regarding flavonoids, ethyl acetate extract (90 mg EQ/g ES) and ethanolic extracts (64 mg EQ/g ES) showed the highest concentrations. For antioxidant activity, ethyl acetate and ethanolic extracts showed significant activity, while for anti-inflammatory activity, ethyl acetate and hexanolic extracts showed more marked activity than ethanolic and dichloromethanic extracts. This study reveals that Tetraclinis articulata leaf extracts are an effective antioxidant and natural anti-inflammatory agent. The results indicate the efficacy of TA leafs extracts from the province of Al Hoceima

Ethnobotanical survey of medicinal plants used in cosmetics in Ketama (North of Morocco)

Medicinal plants serve as a significant source of bioactive molecules, with their utilization in the medical realm dating back to ancient times through herbal preparations. In Morocco, the utilization of plants remains integral to traditional medicine. Despite numerous studies documenting indigenous knowledge regarding plant use, scant attention has been given to northern Morocco, particularly the Rif region. This study seeks to enrich and consolidate our understanding of plant utilization in this area. The ethnobotanical investigation was centred on medicinal plants employed in cosmetics within northern Morocco. A questionnaire was disseminated, gathering data encompassing plant names, utilized plant parts, and preparation methods. The study identified 32 plant species utilized in cosmetics, including Cannabis sativa L., Dittrichia viscosa (L.) Greuter, Juglans regia L., and Rubus fruticosus L. Leaves emerged as the most used plant parts, with decoction and infusion being the predominant preparation methods. This survey facilitated a comprehensive examination of ethnobotanical knowledge, shedding light on the primary plants employed in cosmetics. Prudent management of these resources is imperative to prevent future shortages