Additional file 1 of Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Additional file 1: Fig. S1. Construction of the 30-merUa. Two products (30-merUa1 and 30-merUa2) were obtained (Materials and methods). However, these two products equilibrate with each other and thus could not be isolated separately. In a previous report [7], Dubey, et al. revealed that Ua comprises the α- and β-anomers. The mixture of the two products (C289H370N103O176P29) was confirmed by ESI-MS (m/z 9000.727) and then was used as 30-merUa in our experiment

Additional file 2 of Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Additional file 2: Fig. S2. DNA synthesis across urea (Ua) by Kf exo−. DNA synthesis in Fig. S2 was conducted under the same condition as in Fig. 2C. Kf exo− (75 μU) was incubated with templates containing G (lane 3) or Ua (lane 4) and 100 μM of each of the four dNTPs (lanes 1–4). Lanes 1 and 2 contained no enzyme and are negative controls. The background darkness of panel A is adjusted in Panel B. Fig. S3. DNA synthesis across urea (Ua) by DNA polymerase η. DNA synthesis in Fig. S3 was conducted under the same condition as in Fig. 4B. DNA polymerase η (0.4 ng) was incubated with templates containing G (lane 2) or Ua (lane 4) and 100 μM of each of the four dNTPs (lanes 1, 2, 4 and 5). Lanes 1 and 5 contained no enzyme and are negative controls. The sample in lane 3 was a mixture of the samples in lanes 2 and 4