Data_Sheet_1_The association between parent-child relationship and problematic internet use among English- and Chinese-language studies: A meta-analysis.ZIP

As past studies of the association between parent-child relationship and problematic internet use show mixed results and are influenced by many factors, this meta-analysis of 75 primary Chinese and English language studies from 1990 to 2021 with 110,601 participants (aged 6−25 years) explored (a) the overall association between parent-child relationship and problematic internet use, and (b) whether the association is affected by their types, country, measures, objects of the parent-child relationship, gender, age, year and publication types. We used funnel plots, Classic fail-safe N and Egger's test to test for publication bias and for moderation with the homogeneity tests. The results showed a negative association between quality of parent-child relationship and problematic internet use (r = −0.18, 95% CI = [−0.20, −0.15]). The moderation analysis found that compared with internet addiction tendency, the association between social media addiction and parent-child relationship was stronger. Moreover, the association between the parent-child relationship and problematic internet use of emerging adults (18–25 years old) was stronger than that of adolescents (12−18 years old). Furthermore, the negative association between parent-child relationship and problematic internet use was weaker (a) in Italy than those in Turkey and China, (b) when using CPS (Closeness to Parents Scale), IPPA (Inventory of Parent and Peer Attachment), or PARQ (Parent-Child Relationship Questionnaire) measuring parent-child relationship than using PCCS (Parent-Child Communication Scale), (c) when using IAT measuring problematic internet use rather than using IGDS or APIUS. Hence, these results indicate a negative association between parent-child relationships and problematic internet use, and the association is moderated by types of problematic internet use, age, country, scales of both parent-child relationship and problematic internet use.</p

High-throughput sequencing reveals biofluid exosomal miRNAs associated with immunity in pigs

Large numbers of miRNAs are found in biofluid exosomes. We isolated ~50–200 nm diameter exosomes from four types of porcine biofluid (urine, plasma, semen, and bile) using serial centrifugation and ultracentrifugation procedures. A total of 42.15 M raw data were generated from four small RNA libraries. This produced 40.17 M map-able sequences, of which we identified 204 conserved miRNAs, and 190 novel candidate miRNAs. Furthermore, we identified 34 miRNAs specifically expressed in only one library, all with well-characterized immune-related functions. A set of five universally abundant miRNAs (miR-148a-3p, miR-21-5p, let-7f-5p, let-7i-5p, and miR-99a-5p) across all four biofluids was also found. Function enrichment analysis revealed that the target genes of the five ubiquitous miRNAs are primarily involved in immune and RNA metabolic processes. In summary, our findings suggest that porcine biofluid exosomes contain a large number of miRNAs, many of which may be crucial regulators of the immune system. High-throughput sequencing evidence indicates that porcine biofluid exosomes contain a large number of miRNAs, many of which may be crucial regulators of the immune system.</p

<i>SMY2</i> and <i>SYH1</i> suppress defects in ribosome biogenesis caused by <i>ebp2</i> mutations

<div><p>Ebp2 is an assembly factor of the 60S ribosomal subunit in yeast. We demonstrate that overexpression of <i>SMY2</i> or <i>SYH1</i> partially suppresses defects in growth and ribosome biogenesis of <i>ebp2</i> mutants, and that <i>smy2Δ</i> and <i>syh1Δ</i> exhibit synthetic growth defects with the <i>ebp2</i> allele. These results suggest that Smy2 and Syh1 may be involved in ribosome biogenesis in relation to Ebp2.</p></div

Study of biological up-flow roughing filters designed for drinking water pretreatment in rural areas: using ceramic media as filter material

Roughing filters have been successfully employed as a pretreatment method in rural water supply, but few studies have reported the performance of up-flow roughing filters (URFs) packed with ceramic media. In this study, two pilot-scale URFs filled with ceramic media were designed. Filter performance, height profiles and head loss development were fully investigated. The average DOC, UV254 absorbance, NH4+-N and total bacterial counts removal efficiencies of filters were found to be close to 8%, 10%, 70% and 0.6 log (75%), respectively. Both filters could remove about 60–90% of turbidity with influent turbidity ranging from 1 to 500 NTU and high removal efficiencies (∼60%) were achieved when influent turbidity was lower than 2.5 NTU at a hydraulic load up to 2 m h−1. Height profiles revealed that UV254 absorbance, NH4+-N and turbidity were primarily removed in the former part of filter columns and that the separated solids stored within 20 cm of ceramic media layer above the bottom contributed to over 90% of total head loss. Filter run times were estimated to be around 60–80 days with a maximum head loss of 30 cm and an average influent turbidity of 10 NTU. The results indicated that the two URFs, combining low-cost operation and simple maintenance with good performance, were well suited to small waterworks in rural areas.</p

Profiling the microbial contamination in aviation fuel from an airport

Microbial contamination during fuel storage can cause fuel system fouling and corrosion. Characterizing microbial contamination is critical for preventing and solving these problems. In this study, culture-based combing with the culture-independent methods, were used to profile the microbial contamination in aviation fuel. High-throughput sequencing (HTS) modified by propidium monoazide (PMA) revealed a higher diversity of contaminating microorganisms in samples than the culture method. Proteobacteria (47%), Actinobacteria (21%) and Ascomycota (>99%, fungi) were the most abundant phyla, and the neglected archaea was also detected. Additionally, qPCR-based methods revealed all samples contained a heavy level of microbial contamination, which was more accurate than its culturable counterparts, and fungal contamination was still a problem in aviation fuel. The application of a PCR-based method gives deeper insight into microbial contamination in aviation fuel than the conventional culture method, thus using it for regular detection and accurate description of fuel contamination is strongly recommended in the case of explosive microbial growth.</p