Distribution of CD4+ and antigen-presenting cells in the human endocervix.

<p>a: CD4+, Scale bar: 100 µm. Box depicts zoomed area proximal to columnar epithelium. b: CD11c+, Scale bar: 100 µm. Box depicts zoomed area proximal to columnar epithelium. c: CD68+, Scale bar: 100 µm. Box depicts zoomed area proximal to columnar epithelium. d: CD1a+, Scale bar: 100 µm. Box depicts zoomed area proximal to columnar epithelium. e: CD123+, Scale bar: 50 µm.</p

CD4 expression is present on T cells and antigen-presenting cell subsets.

<p>a: CD4+ (red),CD3+ (green), CD4+CD3+ (yellow), I: 3 µm, Scale bar: 25 µm. b: CD4+ (red), CD1a+ (green), CD4+CD1a+ (yellow), I: 24 µm, Scale bar: 25 µm. c: CD4+ (red), CD11c+ (green), CD4+CD11c+ (yellow), Scale bar: 25 µm. d: CD11c+ (red), CD1a (green), CD11c+CD1a+ (yellow), Scale bar: 25 µm. e: CD123+ (red), CD4+ (green,) CD123+CD4+ (yellow), Scale bar: 25 µm. f: CD123+ (red), BDCA-2+ (green,) CD123+BDCA-2+ (yellow), Scale bar: 25 µm. Arrows indicate single positive cells; arrowheads indicate double positive cells.</p

Endocervical distribution of immune effector cells.

<p>a: CD8+, Scale bar: 100 µm. Box depicts zoomed area proximal to columnar epithelium. b: CD56+, Scale bar: 100 µm. c: CD19+, Scale bar 50 µm. d: IgD+, Scale bar 50 µm.</p

HIV-binding CLRs and CCR5 are expressed in the human endocervix.

<p>a: Langerin+, Scale bar: 100 µm. b: CD11c+ (red), Langerin+ (green), CD11c+ Langerin+ (yellow), Scale bar: 25 µm. c: CD4+ (red), Langerin+ (green), CD4+ Langerin+ (yellow), Scale bar: 25 µm. d: MR+, Scale bar: 100 µm. e: DC-SIGN+, Scale bar: 100 µm. f: MR+ (red),CD68+ (green), MR+CD68+ (yellow), Scale bar: 25 µm. g: DC-SIGN+ (red), CD68+ (green), DC-SIGN+CD68+ (yellow), Scale bar: 25 µm. h: MR+ (red), CD11c+ (green), MR+CD11c+ (yellow), Scale bar: 25 µm. i: DC-SIGN+ (red), CD11c+ (green), DC-SIGN+CD11c+ (yellow), Scale bar: 25 µm. j: CD1a+ (green), CCR5+ (red), Scale bar: 25 µm. k: CD4+ (green), CCR5+ (red), CD4+CCR5+ (yellow), Scale bar: 25 µm. l: CD11c+ (green), CCR5+ (red), CD11c+CCR5+ (yellow), Scale bar: 100 µm. Arrows indicate single positive cells; arrowheads indicate double positive cells.</p

CLR expression on various DC subsets present in the endometrium.

<p>(a) Langerin positive cells were mainly detected within the luminal and glandular epithelium. Positive co-localization (yellow) was detected with Langerin+ cells (green) and (b) CD1a (red) and (c) CD11c (red). (d) Double-positive cells (yellow) expressing Langerin+ (green) and CD4+ (red) cells were also detected within CD4+ lymphoid aggregates. (e) DC-SIGN and (f) MR were detected in the lamina superficialis, just beneath the luminal epithelium (I: minimum distance of cell body to endometrial lumen: 49 µm and 40 µm, respectively). Both (g) DC-SIGN (green) and (h) MR(green) were co-expressed (yellow) on CD68+ cells (red) as well as (i and j, respectively) CD11c+ cells (red). Neither (k) DC-SIGN (red) nor (l) MR (red) were expressed on CD1a+ (green) cells. Scale bars: a: 500 µm; b–d: 25 µm; e–l: 100 µm.</p

The proportional days of fever and days of antibiotic treatment during febrile neutropaenia episodes among A/A (n = 46) and A/O+O/O (n = 41) individuals.

<p> <b>(^*</b><b><i>p</i></b><b><0.05).</b></p

Schematic picture of the uterus.

<p>Schematic picture showing the myometrium and the different components of the human endometrium: I: luminal epithelium; II: lamina superficialis; III: lamina basalis; IV: myometrium and V: glandular epithelium. Quantitative assessment of endometrial tissue samples was performed in all sections but the myometrium.</p

Distribution of antigen-presenting cell subsets in human endometrium.

<p>(a) CD1a+ cells were rare and mainly found within the luminal or glandular epithelium. (b) CD1a+ cells (green) co-expressed (yellow) CD11c+ (red), although not all CD11c+ cells expressed CD1a. (c) The CD1a+ cells (green) also expressed CD4 (red). (d) CD11c+ and (e) CD68+ cells were present in much higher frequencies in both the epithelium and stroma and clusters of both (f) CD11c+ and (g) CD68+ cells were present in the lamina basalis adjacent to glandular epithelium (marked area: 7.1×10⁴ µm² and 8.1×10⁴ µm², respectively). (h) CD123+ cells were solely present in the stroma as scattered cells and in streak formations. (i) Some but not all CD123+ (red) cells co-localized (yellow) with BDCA-2 (green). BDCA-2+ (green) neither co-localized with (j) CD11c+ (red) nor (k) CD68+ (red) cells, although they were occasionally detected in close proximity to each other. Arrowheads indicate example of positive staining. Scale bars: a: 500 µm; b, c, j, k: 25 µm; d–i: 100 µm.</p

MBL concentrations and microbiological findings of febrile and afebrile neutropaenic episodes.

1<p>Comparison of MBL concentrations from episodes of A/A individuals with fever and without fever.</p>2<p>Comparison of MBL concentrations from episodes of A/O and O/O individuals with fever and without fever.</p><p>N.D. Bacteria investigations were not performed as a routine.</p

Plasma concentrations of (A) MBL and (B) CRP, in afebrile and febrile episodes based on pathogen detected.

<p>Comparisons were made between afebrile episodes and febrile episodes with further catergorisation based on microbiological findings. Numbers in the respective groups are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030819#pone-0030819-t002" target="_blank">Table 2</a> (**<i>p</i><0.01, ***<i>p</i><0.001).</p