Additional file 8: Table S7. of Transcriptomic analyses of the radiation response in head and neck squamous cell carcinoma subclones with different radiation sensitivity: time-course gene expression profiles and gene association networks

Significantly enriched pathways of early and late responding genes after irradiation with 8 Gy. Pathways with FDR < 0.1 were considered statistically significant. (XLSX 102 kb

Additional file 5: Table S4. of Transcriptomic analyses of the radiation response in head and neck squamous cell carcinoma subclones with different radiation sensitivity: time-course gene expression profiles and gene association networks

Technical validation of microarray data. Eight of the differentially expressed genes detected with the microarray technology were arbitrarily chosen for technical validation by qRT-PCR. Correlation analysis results (Spearman’s rho coefficient) and fold-changes (microarray and qRT-PCR) are shown. Asterisks (*) indicate fold-change values for genes detected as differentially expressed by microarray analysis. (DOCX 52 kb

Endothelial expression of adhesion and signaling molecules, composition of the vascular wall, and shear rates in the venular microvasculature.

<p>Representative confocal microscopy images of ICAM-1/CD54, VCAM-1/CD106, ICAM-2/CD102, PECAM-1/CD31, JAM-A, and CCL2 expression in venular endothelial cells of the cremaster muscle of WT mice before (open dots) and after (filled dots) stimulation with CCL2 (<b>A</b>; scale bar 50 μm). Panels show quantitative expression levels of these proteins in dependency of the venular diameter as well as the corresponding venular shear rates (mean ± SEM; <i>n</i> = 3–4 per group; #<i>p</i> < 0.05 versus PBS). Panels (<b>B</b>) show representative images and quantitative data for the number of perivascular macrophages, pericytes, and collagen IV LERs of venular microvessels in dependency of the venular diameter (scale bar 50 μm; mean ± SEM; <i>n</i> = 3 per group).</p

Endothelial cell interactions of platelets in the microvasculature.

<p>Interactions of platelets (white) and endothelial cells (blue) were analyzed in the microvasculature of the inflamed cremaster muscle of CX₃CR1^GFP/+ mice by multichannel in vivo microscopy (<b>A</b>; scale bar 100 μm). Panels show results for intravascularly rolling and firmly adherent platelets in venules after 6 h of intrascrotal stimulation with phosphate-buffered saline (PBS) or CCL2 in dependency of the venular diameter (mean ± standard error of the mean [SEM] for <i>n</i> = 4 per group; #<i>p</i> < 0.05 versus PBS). Panel (<b>B;</b> scale bar: 10 μm) shows the relative localization of platelets (white), neutrophils (red), and iMOs (green) to PECAM-1/CD31-immunoreactive endothelial junctions (blue; mean ± SEM for <i>n</i> = 3 per group). Panel (<b>C</b>) shows the adhesion dynamics of platelets, neutrophils, and iMOs during the course of the acute inflammatory response (mean ± SEM for <i>n</i> = 3–4 per group).</p

Role of platelets for the transmigration efficacy of leukocytes.

<p>Representative multichannel in vivo microscopy serial images of intravascularly adherent platelets (white) capturing an intraluminally crawling neutrophil (red) or iMO (green; <b>A</b>; arrows indicate direction of blood flow; scale bar 20 μm). Representative multichannel in vivo microscopy images as well as quantitative analysis of neutrophils and iMOs during their successive extravasation from sites of intravascularly adherent platelets (white; <b>B</b>; mean ± SEM; <i>n</i> = 4 per group; scale bar 20 μm). Panels (<b>C</b>) show quantitative data on the transmigration efficacy of total leukocytes, neutrophils, and iMOs in CCL2-stimulated cremasteric venules of animals receiving platelet-depleting mAbs or isotype control antibodies (mean ± SEM; <i>n</i> = 4 per group; *<i>p</i> < 0.05 versus isotype control).</p

Additional file 12: of Integrative analysis of the microRNA-mRNA response to radiochemotherapy in primary head and neck squamous cell carcinoma cells

Primer sequences for TP53 and EGFR sequencing. (PDF 42 kb

Additional file 7: of Integrative analysis of the microRNA-mRNA response to radiochemotherapy in primary head and neck squamous cell carcinoma cells

Significantly deregulated mRNAs in primary HN2092 after in vitro radiochemotherapy treatment. (PDF 143 kb

Additional file 8: of Integrative analysis of the microRNA-mRNA response to radiochemotherapy in primary head and neck squamous cell carcinoma cells

Pathway enrichment analysis of differentially expressed genes in HN1957 after radiochemotherapy treatment. (PDF 66 kb

Interactions of platelets, neutrophils, and iMOs in the microvasculature.

<p>Interactions of neutrophils (red) and iMOs (green) with endothelial cells (blue) were analyzed in the microvasculature of the cremaster muscle of CX₃CR1^GFP/+ mice by multichannel in vivo microscopy (<b>A</b>; scale bar 100 μm). Panels show results for intravascularly rolling and firmly adherent as well as transmigrated neutrophils or iMOs in venules after 6 h of intrascrotal stimulation with PBS or CCL2 receiving a platelet-depleting anti-CD42b mAb (<b>B</b>), a neutrophil-depleting anti-Ly-6G mAb (<b>C</b>), or isotype control antibodies in dependency of the venular diameter (mean ± SEM for <i>n</i> = 4 per group; #<i>p</i> < 0.05 versus PBS; *<i>p</i> < 0.05 versus isotype control).</p

Graphical synopsis.

<p>Schematic illustration of the sequential steps in the leukocyte recruitment cascade including platelet-directed guidance of leukocytes to their site of extravasation.</p