Characterizing the Circumgalactic Medium of the Lowest-mass Galaxies: A Case Study of IC 1613

Using 10 sight lines observed with the Hubble Space Telescope/Cosmic Origins Spectrograph, we study the circumgalactic medium (CGM) and outflows of IC 1613, which is a low-mass (M_* ~ 10⁸ M_⊙), dwarf irregular galaxy on the outskirts of the Local Group. Among the sight lines, four are pointed toward UV-bright stars in IC 1613, and the other six sight lines are background QSOs at impact parameters from 6 kpc (<0.1R_(200)) to 61 kpc (0.6R_(200)). We detect a number of Si ii, Si iii, Si iv, C ii, and C iv absorbers, most of which have velocities less than the escape velocity of IC 1613 and thus are gravitationally bound. The line strengths of these ion absorbers are consistent with the CGM absorbers detected in dwarf galaxies at low redshifts. Assuming that Si ii, Si iii, and Si iv comprise nearly 100% of the total silicon, we find 3% (~8 × 10³ M_⊙), 2% (~7 × 10³ M_⊙), and 32%–42% [~(1.0–1.3) × 10⁵ M_⊙] of the silicon mass in the stars, interstellar medium, and within 0.6R_(200) of the CGM of IC 1613. We also estimate the metal outflow rate to be Ṁ_(out,Z) ⩾ 1.1 x 10⁻⁵ M_⊙ yr⁻¹ and the instantaneous metal mass loading factor to be η_Z ≥ 0.004, which are in broad agreement with available observation and simulation values. This work is the first time a dwarf galaxy of such low mass is probed by a number of both QSO and stellar sight lines, and it shows that the CGM of low-mass, gas-rich galaxies can be a large reservoir enriched with metals from past and ongoing outflows

An in vitro proof-of-principle study of sonobactericide

Infective endocarditis (IE) is associated with high morbidity and mortality rates. The predominant bacteria causing IE is Staphylococcus aureus (S. aureus), which can bind to existing thrombi on heart valves and generate vegetations (biofilms). In this in vitro flow study, we evaluated sonobactericide as a novel strategy to treat IE, using ultrasound and an ultrasound contrast agent with or without other therapeutics. We developed a model of IE biofilm using human whole-blood clots infected with patient-derived S. aureus (infected clots). Histology and live-cell imaging revealed a biofilm layer of fibrin-embedded living Staphylococci around a dense erythrocyte core. Infected clots were treated under flow for 30 minutes and degradation was assessed by time-lapse microscopy imaging. Treatments consisted of either continuous plasma flow alone or with different combinations of therapeutics: oxacillin (antibiotic), recombinant tissue plasminogen activator (rt-PA; thrombolytic), intermittent continuous-wave low-frequency ultrasound (120-kHz, 0.44 MPa peak-to-peak pressure), and an ultrasound contrast agent (Definity). Infected clots exposed to the combination of oxacillin, rt-PA, ultrasound, and Definity achieved 99.3 ± 1.7% loss, which was greater than the other treatment arms. Effluent size measurements suggested low likelihood of emboli formation. These results support the continued investigation of sonobactericide as a therapeutic strategy for IE

The Highly Unusual Chemical Composition of the Hercules Dwarf Spheroidal Galaxy

We report on the abundance analysis of two red giants in the faint Hercules dwarf spheroidal (dSph) galaxy. These stars show a remarkable deficiency in the neutron-capture elements, while the hydrostatic alpha-elements (O, Mg) are strongly enhanced. Our data indicate [Ba/Fe] and [Mg/Fe] abundance ratios of <-2 dex and ~+0.8 dex, respectively, with essentially no detection of other n-capture elements. In contrast to the only other dSph star with similar abundance patterns, Dra 119, which has a very low metallicity at [Fe/H]=-2.95 dex, our objects, at [Fe/H]~-2.0 dex, are only moderately metal poor. The measured ratio of hydrostatic/explosive alpha-elements indicates that high-mass (~35 M_sun) Type II supernovae progenitors are the main, if not only, contributors to the enrichment of this galaxy. This suggests that star formation and chemical enrichment in the ultrafaint dSphs proceeds stochastically and inhomogeneously on small scales, or that the IMF was strongly skewed to high mass stars. The neutron capture deficiencies and the [Co/Fe] and [Cr/Fe] abundance ratios in our stars are similar to those in the extremely low metallicity Galactic halo. This suggests that either our stars are composed mainly of the ejecta from the first, massive, population III stars (but at moderately high [Fe/H]), or that SN ejecta in the Hercules galaxy were diluted with ~30 times less hydrogen than typical for extreme metal-poor stars.Comment: 5 pages, 3 figures, accepted by Astrophysical Journal Letter

Tentative detection of the circumgalactic medium of the isolated low-mass dwarf galaxy WLM

We report a tentative detection of the circumgalactic medium (CGM) of Wolf–Lundmark–Melotte (WLM), an isolated, low-mass (logM*/M⊙ ≈ 7.6), dwarf irregular galaxy in the Local Group (LG). We analyse an HST/COS archival spectrum of a quasar sightline (PHL2525), which is 45 kpc (0.5 virial radius) from WLM and close to the Magellanic Stream (MS). Along this sightline, two ion absorbers are detected in Si II, Si III, Si IV, C II, and C IV at velocities of ∼−220 km s⁻¹ (Component v-220) and ∼−150 km s⁻¹ (Component v-150). To identify their origins, we study the position–velocity alignment of the components with WLM and the nearby MS. Near the magellanic longitude of PHL2525, the MS-related neutral and ionized gas moves at ≲−190 km s⁻¹, suggesting an MS origin for Component v-220, but not for Component v-150. Because PHL2525 passes near WLM and Component v-150 is close to WLM’s systemic velocity (∼−132 km s⁻¹), it is likely that Component v-150 arises from the galaxy’s CGM. This results in a total Si mass in WLM’s CGM of M^(CGM)_(Si)∼(0.2−1.0)×10⁵ M⊙ using assumption from other COS dwarf studies. Comparing M^(CGM)_(Si) to the total Si mass synthesized in WLM over its lifetime (∼1.3 × 10⁵ M⊙), we find ∼3 per cent is locked in stars, ∼6 per cent in the ISM, ∼15–77 per cent in the CGM, and the rest (∼14–76 per cent) is likely lost beyond the virial radius. Our finding resonates with other COS dwarf galaxy studies and theoretical predictions that low-mass galaxies can easily lose metals into their CGM due to stellar feedback and shallow gravitational potential

Synthesis and structural characterization of a mimetic membrane-anchored prion protein

During pathogenesis of transmissible spongiform encephalopathies (TSEs) an abnormal form (PrPSc) of the host encoded prion protein (PrPC) accumulates in insoluble fibrils and plaques. The two forms of PrP appear to have identical covalent structures, but differ in secondary and tertiary structure. Both PrPC and PrPSc have glycosylphospatidylinositol (GPI) anchors through which the protein is tethered to cell membranes. Membrane attachment has been suggested to play a role in the conversion of PrPC to PrPSc, but the majority of in vitro studies of the function, structure, folding and stability of PrP use recombinant protein lacking the GPI anchor. In order to study the effects of membranes on the structure of PrP, we synthesized a GPI anchor mimetic (GPIm), which we have covalently coupled to a genetically engineered cysteine residue at the C-terminus of recombinant PrP. The lipid anchor places the protein at the same distance from the membrane as does the naturally occurring GPI anchor. We demonstrate that PrP coupled to GPIm (PrP-GPIm) inserts into model lipid membranes and that structural information can be obtained from this membrane-anchored PrP. We show that the structure of PrP-GPIm reconstituted in phosphatidylcholine and raft membranes resembles that of PrP, without a GPI anchor, in solution. The results provide experimental evidence in support of previous suggestions that NMR structures of soluble, anchor-free forms of PrP represent the structure of cellular, membrane-anchored PrP. The availability of a lipid-anchored construct of PrP provides a unique model to investigate the effects of different lipid environments on the structure and conversion mechanisms of PrP

Bridging the gap between low and high mass dwarf galaxies

While the dark matter content within the most massive giant and smallest dwarf galaxies has been probed -- spanning a range of over one million in mass -- an important observational gap remains for galaxies of intermediate mass. This gap covers K band magnitudes of approximately -16 > M_K > -18 (for which dwarf galaxies have B--K ~ 2). On the high mass side of the gap are dwarf elliptical (dE) galaxies, that are dominated by stars in their inner regions. While the low mass side includes dwarf spheroidal (dSph) galaxies that are dark matter-dominated and ultra compact dwarf (UCD) objects that are star-dominated. Evolutionary pathways across the gap have been suggested but remain largely untested because the `gap' galaxies are faint, making dynamical measurements very challenging. With long exposures on the Keck telescope using the ESI instrument we have succeeded in bridging this gap by measuring the dynamical mass for five dwarf galaxies with M_K ~ -17.5 (M_B ~ --15.5). With the exception of our brightest dwarf galaxy, they possess relatively flat velocity dispersion profiles of around 20 km/s. By examining their 2D scaling relations and 3D fundamental manifold, we found that the sizes and velocity dispersions of these gap galaxies reveal continuous trends from dE to dSph galaxies. We conclude that low-luminosity dwarf elliptical galaxies are dominated by stars, not by dark matter, within their half light radii. This finding can be understood if internal feedback processes are operating most efficiently in gap galaxies, gravitationally heating the centrally-located dark matter to larger radii. Whereas external environmental processes, which can strip away stars, have a greater influence on dSph galaxies resulting in their higher dark matter fractions. Abridged.Comment: 20 pages, includes 12 figures, accepted for publication in MNRA