9 research outputs found
Shared and unique OTUs of the PCR-based and shotgun-based sponge datasets.
<p>Circle sizes are proportional to OTU number. (A) 0.01 phylogenetic distance OTU. (B) 0.03 phylogenetic distance OTU. (C) 0.05 phylogenetic distance OTU.</p
Abundance and primer-mismatches in the top OTUs at the 0.01 phylogenetic distance level for the sponge datasets.
<p>Asterisk, primer-mis-match event.</p
16S rRNA gene contigs and chimeric contigs for simulated datasets.
<p>Open circle: non-chimeric contigs; solid circle: chimeric contigs containing one contaminating read; solid triangles: chimeric contigs containing more than one contaminating read. Arrow: chimera detected by UChime. (A) HC. (B) MC. (C) LC.</p
Reads, 16S rRNAcontigs, OTUs and chimera examination of the simulated communities.
<p>Reads, 16S rRNAcontigs, OTUs and chimera examination of the simulated communities.</p
Taxonomic classification of assembled and unassembled shotgun 16S rRNA gene reads for simulated datasets.
<p>(A) HC. (B) MC. (C) LC.</p
Phylum-level classification of the sponge pyro-tag-sequencing and shotgun sequencing datasets.
<p>(A) 16S rRNA gene PCR approach. (B) Unassembled shotgun 16S rRNA gene reads. (C) Assembled shotgun 16S rRNA gene reads. (D) Single-copy gene analysis.</p
