Bigradient Phase Referencing

We propose bigradient phase referencing (BPR), a new radio-observation technique, and report its performance using the Japanese very-long-baseline-interferometry network (JVN). In this method, a weak source is detected by phase-referencing using a primary calibrator, in order to play a role as a secondary calibrator for phase-referencing to a weak target. We will be given the opportunity to select a calibrator from lots of milli-Jansky sources, one of which may be located at the position closer to the target. With such a smaller separation, high-quality phase-referencing can be achieved. Furthermore, a subsequent more-sophisticated calibration can relocate array's focus to a hypothetical point much closer to the target; a higher quality of phase referencing is available. Our demonstrative observations with strong radio sources have proved the capabilities of BPR in terms of image dynamic ranges and astrometric reproducibility. The image dynamic range on a target has been improved with a factor of about six compared to that of normal phase-referencing; the resultant position difference of target's emission between two epochs was only 62+-50 micro-arcsecond, even with less than 2300-km baselines at 8.4 GHz and fast-switching of a target-calibrator pair of a 2.1-degree separation.Comment: 10 pages, 4 figures, accepted for publication in PAS

Multifrequency VLBI Observations of the Broad Absorption Line Quasar J1020+4320: Recently Restarted Jet Activity?

This paper reports very-long-baseline interferometry observations of the radio-loud broad absorption line (BAL) quasar J1020+4320 at 1.7, 2.3, 6.7, and 8.4 GHz using the Japanese VLBI network (JVN) and European VLBI network (EVN). The radio morphology is compact with a size of ~10 pc. The convex radio spectrum is stable over the last decade; an observed peak frequency of 3.2 GHz is equivalent to 9.5 GHz in the rest frame, suggesting an age of the order of ~100 years as a radio source, according to an observed correlation between linear size and peak frequency of compact steep spectrum (CSS) and giga-hertz peaked spectrum (GPS) radio sources. A low-frequency radio excess suggests relic of past jet activity. J1020+4320 may be one of the quasars with recurrent and short-lived jet activity during a BAL-outflowing phase.Comment: 7 pages, 2 figures, 2 tables, accepted for publication in PAS

Japanese VLBI Network observations of radio-loud narrow-line Seyfert 1 galaxies

We performed phase-reference very long baseline interferometry (VLBI) observations on five radio-loud narrow-line Seyfert 1 galaxies (NLS1s) at 8.4 GHz with the Japanese VLBI Network (JVN). Each of the five targets (RXS J08066+7248, RXS J16290+4007, RXS J16333+4718, RXS J16446+2619, and B3 1702+457) in milli-Jansky levels were detected and unresolved in milli-arcsecond resolutions, i.e., with brightness temperatures higher than 10^7 K. The nonthermal processes of active galactic nuclei (AGN) activity, rather than starbursts, are predominantly responsible for the radio emissions from these NLS1s. Out of the nine known radio-loud NLS1s, including the ones chosen for this study, we found that the four most radio-loud objects exclusively have inverted spectra. This suggests a possibility that these NLS1s are radio-loud due to Doppler beaming, which can apparently enhance both the radio power and the spectral frequency.Comment: 8 pages, 2 figures, accepted for publication in PAS

Mapping Observations of 6.7 GHz Methanol Masers with Japanese VLBI Network

We have observed 13 methanol maser sources associated with massive star-forming regions; W3(OH), Mon R2, S 255, W 33A, IRAS 18151-1208, G 24.78+0.08, G 29.95-0.02, IRAS 18556+0136, W 48, OH 43.8-0.1, ON 1, Cep A and NGC 7538 at 6.7 GHz using the Japanese VLBI Network (JVN). Twelve of the thirteen sources were detected at our longest baseline of $\sim$50 M$\lambda$, and their images are presented. Seven of them are the first VLBI images at 6.7 GHz. This high detection rate and the small fringe spacing of $\sim$4 milli-arcsecond suggest that most of the methanol maser sources have compact structure. Given this compactness as well as the known properties of long-life and small internal-motion, this methanol maser line is suitable for astrometry with VLBI.Comment: 15 pages, 13 figures, accepted for publication in PAS

VLBI Detections of Parsec-Scale Nonthermal Jets in Radio-Loud Broad Absorption Line Quasars

We conducted radio detection observations at 8.4 GHz for 22 radio-loud broad absorption line (BAL) quasars, selected from the Sloan Digital Sky Survey (SDSS) Third Data Release, by a very-long-baseline interferometry (VLBI) technique. The VLBI instrument we used was developed by the Optically ConnecTed Array for VLBI Exploration project (OCTAVE), which is operated as a subarray of the Japanese VLBI Network (JVN). We aimed at selecting BAL quasars with nonthermal jets suitable for measuring their orientation angles and ages by subsequent detailed VLBI imaging studies to evaluate two controversial issues of whether BAL quasars are viewed nearly edge-on, and of whether BAL quasars are in a short-lived evolutionary phase of quasar population. We detected 20 out of 22 sources using the OCTAVE baselines, implying brightness temperatures greater than 10^5 K, which presumably come from nonthermal jets. Hence, BAL outflows and nonthermal jets can be generated simultaneously in these central engines. We also found four inverted-spectrum sources, which are interpreted as Doppler-beamed, pole-on-viewed relativistic jet sources or young radio sources: single edge-on geometry cannot describe all BAL quasars. We discuss the implications of the OCTAVE observations for investigations for the orientation and evolutionary stage of BAL quasars.Comment: 10 pages, no figure, 3 tables, accepted for publication in PAS

Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment

Ability to form cellular aggregations such as tumorspheres and spheroids have been used as a morphological marker of malignant cancer cells and in particular cancer stem cells (CSC). However, the common definition of the types of cellular aggregation formed by cancer cells has not been available. We examined morphologies of 67 cell lines cultured on three dimensional morphology enhancing NanoCulture Plates (NCP) and classified the types of cellular aggregates that form. Among the 67 cell lines, 49 cell lines formed spheres or spheroids, 8 cell lines formed grape-like aggregation (GLA), 8 cell lines formed other types of aggregation, and 3 cell lines formed monolayer sheets. Seven GLA-forming cell lines were derived from adenocarcinoma among the 8 lines. A neuroendocrine adenocarcinoma cell line PC-3 formed asymmetric GLA with ductal structures on the NCPs and rapidly growing asymmetric tumors that metastasized to lymph nodes in immunocompromised mice. In contrast, another adenocarcinoma cell line DU-145 formed spheroids in vitro and spheroid-like tumors in vivo that did not metastasize to lymph nodes until day 50 after transplantation. Culture in the 3D nanoenvironment and in a defined stem cell medium enabled the neuroendocrine adenocarcinoma cells to form slowly growing large organoids that expressed multiple stem cell markers, neuroendocrine markers, intercellular adhesion molecules, and oncogenes in vitro. In contrast, the more commonly used 2D serum-contained environment reduced intercellular adhesion and induced mesenchymal transition and promoted rapid growth of the cells. In addition, the 3D stemness nanoenvironment promoted secretion of HSP90 and EpCAM-exosomes, a marker of CSC phenotype, from the neuroendocrine organoids. These findings indicate that the NCP-based 3D environment enables cells to form stem cell tumoroids with multipotency and model more accurately the in vivo tumor status at the levels of morphology and gene expression

Moiré superlattice and two-dimensional free-electron-like states of indium triple-layer structure on Si(111)

We studied the growth of an indium triple-atomic-layer film and the two-dimensional free-electron-like electronic states on Si(111) by low-energy electron diffraction (LEED), scanning tunneling microscopy (STM), and angle-resolved photoelectron spectroscopy (ARPES). By depositing In on the In/Si(111)- √ 7 × √ 3-rect surface below 100 K, followed by brief postannealing up to 140 K, we successfully obtained well-crystalline films exhibiting sharp superstructure LEED spots. We revealed an (11 × 11) superlattice of the triple-layer structure, while both LEED and STM showed a (5.5 × 5.5) pseudoperiodicity. This pseudoperiodicity was attributed to the moiré interference between the Si(111)-(11 × 11) lattice (a = 3.84 Å) and the In (13 × 13) hexagonal lattice, which has a lattice constant of 3.25 Å, with the ratio very close to 13/11. ARPES measurements unveiled two free-electron-like states with Fermi wave vectors of 1.32 and 1.46 Å⁻¹. We also observed replica Fermi surfaces, which are associated with the reciprocal lattice vectors of both the (1 × 1) Si(111) and the In hexagonal layers. This further confirms the hexagonal atomic arrangement of the In triple-layer structure

Ninjinyoeito Improves Behavioral Abnormalities and Hippocampal Neurogenesis in the Corticosterone Model of Depression

Ninjinyoeito (NYT), a traditional Chinese medicine consisting of 12 herbs, is designed to improve fatigue, cold limbs, anorexia, night sweats, and anemia. Recently, NYT was reported to improve cognitive outcome and depression in patients with Alzheimer’s disease. However, little is known about how NYT alleviates depression and cognitive dysfunction. In this study, we investigated the effects and mechanisms of NYT in a corticosterone (CORT)-induced model of depression. Chronic NYT treatment ameliorated the depressive-like behaviors induced by CORT treatment in three types of behavioral tests. In addition, chronic NYT treatment also improved memory disruptions induced by CORT in both the Y-maze and novel object recognition tests, without affecting locomotor activity. Furthermore, we also showed that NYT treatment attenuated the CORT-induced reduction in cell proliferation and immature neuronal cell numbers in mouse hippocampal dentate gyrus. These results suggest that NYT has therapeutic effects on CORT-induced behavioral abnormalities and inhibition of hippocampal neurogenesis