Modelling nonlinear ultrasound propagation in absorbing media using the k-Wave toolbox: Experimental validation

This data was collected to validate simulations performed using the k-Wave toolbox when the wave propagation is nonlinear and the medium is absorbing. Experiments were conducted using a diagnostic ultrasound scanner (Sonix RP, Ultrasonix) with a linear array probe (L9-4/38) in both water and olive oil. Measurements were performed using a two-axis automated scanning tank with a 0.4 mm membrane hydrophone.  The coordinate system used is as follows: X is the elevation direction relative to the transducer (out-of-plane), Y is the lateral (in-plane) direction, Z is the axial direction. In total, this study contains 12 datasets contained in 4 files as follows. File 1. X-Y scans of transducer in water with no steering (4 datasets with planes measured at 1.3, 11.1, 21.3, and 31.3 mm from the transducer). File 2. X-Y scans of transducer in water with a steering angle of 20 degrees (2 datasets with planes measured at 1.3 and 21.3 mm from the transducer). File 3. Y-Z scans of the transducer in water with steering angles of 0 (no steering) and 20 degrees (2 datasets). File 4. X-Y scans of transducer in olive oil with no steering (4 datasets with planes measured at 2.4, 12.4, 22.4, 32.4 mm from the transducer). A MATLAB script is provided to produce simple summary plots of the data. Notes: For all X-Y scans, the reported Z-positions are approximate. Z-alignment was performed using a manual translation stage, and the position of the transducer relative to the hydrophone in the first scan plane was estimated using a diagnostic ultrasound image. A more accurate estimate could potentially be obtained using the time-of-flight data for the recorded waveforms. For dataset 3, the reported X-Y coordinate origin for the scan plane at 2.39mm (dataset1) is not aligned with the X-Y coordinate origin for the other scan planes (dataset2, dataset3, dataset4). This could potentially be corrected, e.g., using acoustic holography and aligning the projections to the measured planes. </p

Supplementary document for A single optical element to generate meter-scale THz diffraction-free beam - 6082331.pdf

Supplemental material</p

Supplementary document for Generation of terahertz quasi-Pearcey beam and its investigation in ptychography - 6689974.pdf

Derivation of the Eq. (5

Novel Biphasically and Reversibly Transparent Phase Change Material to Solve the Thermal Issues in Transparent Electronics

Highly integrated transparent electronic systems are experiencing significant thermal bottlenecks due to the rapid growth of transparent electronics and the lack of suitable transparent thermal management solutions. Therefore, transparent thermal management materials are highly desirable in modern transparent electronics. Based on the phase change properties of polyethylene glycol (PEG) and the encapsulable properties of epoxy resin (EP), we synthesize a biphasically and reversibly transparent PEG/EP composite for thermal energy storage (TPE–TES). Energy-driven structural rearrangements in cross-linked networks are responsible for the high transparency with practical thickness. According to SEM and TEM investigations, PEG and EP achieve submicron phase dispersion, while TPE–TES forms a smooth and continuous surface that suppresses diffuse reflections and contributes to improved visible light penetration. The unique combination of phase change and optical transparency gives TPE–TES the ability to regulate thermal storage, rapid temperature change, and spatial temperature uniformity of transparent electronics. Due to its flexibility, stability, and processability, TPE–TES is also suitable and ideal as thin surface coating films or thick transparent flexible substrates for a wide range of applications in the integration of electronic devices

MOESM4 of Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction

Additional file 4: Table S3. The distribution of V-J combinations in each sample

Additional file 1 of Identification of SARS-CoV-2-specific T cell and its receptor

Supplementary Material

MOESM7 of Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction

Additional file 7: Table S6. The distribution of AA usage in CDR3

Additional file 1 of Extracellular vesicles from Lactobacillus druckerii inhibit hypertrophic scar fibrosis

Additional file 1: Figure S1. Transmission electron microscopy imaging of LDEVs (scale bar = 200 nm). Figure S2. Nanoparticle tracking analysis of the sample from PBS. Figure S3. Representative images of α-SMA immunofluorescence staining in HFBs stimulated with LDEVs. scale bar = 500 μm. Figure S4. Representative images of immunofluorescence staining of Ki-67 in HFBs and NFBs exposure to LDEVs or PBS, scale bar = 125 μm. Figure S5. Quantification Ki-67 positive cells from NFBs and HFBs after PBS and LDEVs treatment. Figure S6. LDEVs inhibit HFBs proliferation according to CCK8 assay (A) and Transwell assay (B). Figure S7. qRT-PCR analysis of the fibrosis related factors (Collagen I, Collagen III and α-SMA) in HFBs treated with LDEVs. Figure S8. The expression of inflammatory factors (IL-1β, TNF-α, and IL-6) in RAW264.7 cells and the cell supernatants of RAW264.7 cells after LDEVs treatment. Figure S9. Representative images of wounds treated with PBS, LDEVs at days 0, 3, 6, 9, 12 and 15 post-wounding. Figure S10. The influence of LDEVs on the expression of MAPK signaling pathway in HFBs and NFBs

Data_Sheet_1_T Cell Receptor Immune Repertoires Are Promptly Reconstituted After Methicillin-Resistant Staphylococcus aureus Infection.PDF

T cells represent a subset of lymphocytes characterized by immunosurveillance and immunoregulation function. Peripheral blood mononuclear cells (PBMCs) are enriched in T cells, which exert critical antimicrobial roles in infectious diseases. High-throughput sequencing of the T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. Flow cytometry was used to analyse the distribution of αβ/γδ T cells and their CD69, IFN-γ/IL-17 expression from PBMCs. Here, we utilized next-generation sequencing (NGS) to detect the complementarity determining region 3 (CDR3) of TCRβ (TRB) and TCRδ (TRD) chain after methicillin-resistant Staphylococcus aureus (MRSA) infection. Our data demonstrated a significant increase in the activation of αβ and γδ T cells after MRSA infection. Simultaneously, significantly high CDR3 amino acid (AA) diversity and markedly reconstituted TCR immune repertoires were observed after MRSA infection. Finally, we identified several MRSA-specific initial CDR3 AA motifs after MRSA infection. Our work reveals the profiles of TRB and TRD immune repertoires in response to MRSA and demonstrates a reconstitution of the TCR immune repertoire after MRSA infection.</p

Table_3_T Cell Receptor Immune Repertoires Are Promptly Reconstituted After Methicillin-Resistant Staphylococcus aureus Infection.XLSX

T cells represent a subset of lymphocytes characterized by immunosurveillance and immunoregulation function. Peripheral blood mononuclear cells (PBMCs) are enriched in T cells, which exert critical antimicrobial roles in infectious diseases. High-throughput sequencing of the T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. Flow cytometry was used to analyse the distribution of αβ/γδ T cells and their CD69, IFN-γ/IL-17 expression from PBMCs. Here, we utilized next-generation sequencing (NGS) to detect the complementarity determining region 3 (CDR3) of TCRβ (TRB) and TCRδ (TRD) chain after methicillin-resistant Staphylococcus aureus (MRSA) infection. Our data demonstrated a significant increase in the activation of αβ and γδ T cells after MRSA infection. Simultaneously, significantly high CDR3 amino acid (AA) diversity and markedly reconstituted TCR immune repertoires were observed after MRSA infection. Finally, we identified several MRSA-specific initial CDR3 AA motifs after MRSA infection. Our work reveals the profiles of TRB and TRD immune repertoires in response to MRSA and demonstrates a reconstitution of the TCR immune repertoire after MRSA infection.</p