Comparison of Global-τFCS analysis with known R6G and RhB sample mixtures.

<p>Recovery of molecular concentrations across nine known concentration ratios using Global-τFCS. Calibrated known concentrations (solid lines) compared to recovered concentrations of R6G (red circles) and RhB (blue circles). Data points and error bars represent the average and standard deviation of three repeated experiments.</p

τFCS analysis of binary dye mixtures with known R6G and RhB concentrations.

<p>(a) Recovery of molecular concentrations across nine known concentration ratios using τFCS. Measured concentrations are shown as red and blue squares, and the solid lines show the known concentration of each dye mixture. (b) Sample parameters detailing concentrations, diffusion coefficients, molecular brightnesses and fluorescence lifetimes of the prepared R6G and RhB mixtures. Recovery of RhB diffusion coefficient (c), molecular brightness (d), and fluorescence lifetime (e) respectively. Data points and error bars represent the average and standard deviation of three repeated experiments.</p

Identifying a second component from simulated mixture data <i>via</i> incorrect single component analysis.

<p>These curves shown represent the chi-squared contours for the different fitting methods when fitting two-component data with a single component model. When the χ² value exceeds ∼1.3 we conclude that the single component analysis fails, indicating the presence of a second component in the sample. Four χ² surfaces demonstrate the boundary in parameter space for 1) FCS (green lines); 2) Fluorescence Lifetime (yellow lines); 3) Fluorescence Lifetime with average intensity constraint (blue lines); and 4) τFCS with average intensity constraint (red lines).</p

Molecular parameter values recovered using Global-τFCS compared to known parameter values.

<p>The accuracy of the recovered information goes well beyond previous experimental capabilities, which is more remarkable given that no fitting constraints or <i>a priori</i> assumptions were required for these fitting results.</p

Direct Observation of Nucleation and Growth in Amyloid Self-Assembly

Direct Observation of Nucleation and Growth in Amyloid Self-Assembl

Direct Observation of Nucleation and Growth in Amyloid Self-Assembly

Direct Observation of Nucleation and Growth in Amyloid Self-Assembl

Direct Observation of Nucleation and Growth in Amyloid Self-Assembly

Direct Observation of Nucleation and Growth in Amyloid Self-Assembl