Table_1_Low-coverage whole genome sequencing of diverse Dioscorea bulbifera accessions for plastome resource development, polymorphic nuclear SSR identification, and phylogenetic analyses.docx

Dioscorea bulbifera (Dioscoreaceae), a versatile herbaceous climber native to Africa and Asia, holds significant nutritional and medicinal value. Despite extensive characterization and genetic variability analyses of African accessions, studies on the genetic variation of this species in China are limited. To address this gap, we conducted low-coverage whole genome sequencing on D. bulbifera accessions from diverse regions across mainland China and Taiwan island. Our initial investigation encompassed comprehensive comparative plastome analyses of these D. bulbifera accessions, and developing plastome resources (including plastome-derived repetitive sequences, SSRs, and divergent hotspots). We also explored polymorphic nuclear SSRs and elucidated the intraspecific phylogeny of these accessions. Comparative plastome analyses revealed that D. bulbifera plastomes exhibited a conserved quadripartite structure with minimal size variation mainly attributed to intergenic spacer regions, reinforcing prior observations of a high degree of conservation within a species. We identified 46 to 52 dispersed repeats and 151 to 163 plastome-derived SSRs, as well as highlighted eight key divergent hotspots in these D. bulbifera accessions. Furthermore, we developed 2731 high-quality candidate polymorphic nuclear SSRs for D. bulbifera. Intraspecific phylogenetic analysis revealed three distinct clades, where accessions from Southeast China formed a sister group to those from South China and Taiwan island, and collectively, these two clades formed a sister group to the remaining accessions, indicating potential regional genetic divergence. These findings not only contributed to the understanding of the genetic variation of D. bulbifera, but also offered valuable resources for future research, breeding efforts, and utilization of this economically important plant species.</p

Table_3_Low-coverage whole genome sequencing of diverse Dioscorea bulbifera accessions for plastome resource development, polymorphic nuclear SSR identification, and phylogenetic analyses.xlsx

Dioscorea bulbifera (Dioscoreaceae), a versatile herbaceous climber native to Africa and Asia, holds significant nutritional and medicinal value. Despite extensive characterization and genetic variability analyses of African accessions, studies on the genetic variation of this species in China are limited. To address this gap, we conducted low-coverage whole genome sequencing on D. bulbifera accessions from diverse regions across mainland China and Taiwan island. Our initial investigation encompassed comprehensive comparative plastome analyses of these D. bulbifera accessions, and developing plastome resources (including plastome-derived repetitive sequences, SSRs, and divergent hotspots). We also explored polymorphic nuclear SSRs and elucidated the intraspecific phylogeny of these accessions. Comparative plastome analyses revealed that D. bulbifera plastomes exhibited a conserved quadripartite structure with minimal size variation mainly attributed to intergenic spacer regions, reinforcing prior observations of a high degree of conservation within a species. We identified 46 to 52 dispersed repeats and 151 to 163 plastome-derived SSRs, as well as highlighted eight key divergent hotspots in these D. bulbifera accessions. Furthermore, we developed 2731 high-quality candidate polymorphic nuclear SSRs for D. bulbifera. Intraspecific phylogenetic analysis revealed three distinct clades, where accessions from Southeast China formed a sister group to those from South China and Taiwan island, and collectively, these two clades formed a sister group to the remaining accessions, indicating potential regional genetic divergence. These findings not only contributed to the understanding of the genetic variation of D. bulbifera, but also offered valuable resources for future research, breeding efforts, and utilization of this economically important plant species.</p

Table_2_Low-coverage whole genome sequencing of diverse Dioscorea bulbifera accessions for plastome resource development, polymorphic nuclear SSR identification, and phylogenetic analyses.xlsx

Dioscorea bulbifera (Dioscoreaceae), a versatile herbaceous climber native to Africa and Asia, holds significant nutritional and medicinal value. Despite extensive characterization and genetic variability analyses of African accessions, studies on the genetic variation of this species in China are limited. To address this gap, we conducted low-coverage whole genome sequencing on D. bulbifera accessions from diverse regions across mainland China and Taiwan island. Our initial investigation encompassed comprehensive comparative plastome analyses of these D. bulbifera accessions, and developing plastome resources (including plastome-derived repetitive sequences, SSRs, and divergent hotspots). We also explored polymorphic nuclear SSRs and elucidated the intraspecific phylogeny of these accessions. Comparative plastome analyses revealed that D. bulbifera plastomes exhibited a conserved quadripartite structure with minimal size variation mainly attributed to intergenic spacer regions, reinforcing prior observations of a high degree of conservation within a species. We identified 46 to 52 dispersed repeats and 151 to 163 plastome-derived SSRs, as well as highlighted eight key divergent hotspots in these D. bulbifera accessions. Furthermore, we developed 2731 high-quality candidate polymorphic nuclear SSRs for D. bulbifera. Intraspecific phylogenetic analysis revealed three distinct clades, where accessions from Southeast China formed a sister group to those from South China and Taiwan island, and collectively, these two clades formed a sister group to the remaining accessions, indicating potential regional genetic divergence. These findings not only contributed to the understanding of the genetic variation of D. bulbifera, but also offered valuable resources for future research, breeding efforts, and utilization of this economically important plant species.</p

Light Excitation of a Bismuth Iodide Complex Initiates I–I Bond Formation Reactions of Relevance to Solar Energy Conversion

The titration of iodide into acetonitrile solutions of BiI₃ resulted in the formation of [BiI₆]^3–. Ligand-to-metal charge transfer (LMCT) excitation of [BiI₆]^3– yielded a transient species assigned as the diiodide anion I₂^•– directly ligated to Bi, [Bi­(I₂^•–)­I_<i>x</i>]^<i>n</i>. With 20 ns time resolution, transient absorption measurements revealed the appearance of two species assigned on the analysis of the iodine molecular orbitals as an η² ligated I₂^•–, [(η²-I₂)­BiI₄]^3– (λ_max = 640 nm), and an η¹ species [(η¹-I₂)­BiI₄]^3– (λ_max = 750 nm). The rapid appearance of this intermediate was attributed to intramolecular I–I bond formation. The [(η²-I₂)­BiI₄]^3– subsequently reacted with 1 equiv of iodide to yield [(η¹-I₂)­BiI₅]^4–. Interestingly, [(η¹-I₂)­BiI₅]^4– decayed to ground state products with a first-order rate constant of <i>k</i> = 2 × 10³ s^–1. Under the same experimental conditions, I₂^•– in CH₃CN rapidly disproportionates with a tremendous loss of free energy, Δ<i>G</i>^o = −2.6 eV. The finding that metal ligation inhibits this energy wasting reaction is of direct relevance to solar energy conversion. The photochemistry itself provides a rare example of one electron oxidized halide species coordinated to a metal ion of possible relevance to reductive elimination/oxidation addition reaction chemistry of transition metal catalysts

The value of squamous cell carcinoma antigen (SCCa) to determine the lymph nodal metastasis in cervical cancer: A meta-analysis and literature review

<div><p>Background</p><p>The diagnostic power of CT or MRI on the lymph node status was limited. Supplement measurements were needed to assist the diagnosis of lymph node metastasis. The SCCa was reported to be close related to lymph node status. But currently the clinical value of serum SCCa measurement in lymph node status has not been clearly defined. This meta-analysis was to investigate this topic on a large scale.</p><p>Method</p><p>Searching the Pubmed, Embase, Cochrane library, CNKI and Wanfang database for SCC-Ag/SCCA/SCC-antigen and cervical cancer/tumor/carcinoma/neoplasm published in any language from Jan 1 1990 to Aug 1 2017. QUADAS (quality assessment of diagnostic accuracy studies) was used to evaluate the quality of the articles. An eligible set of data should include true positive, true negative, false positive and false negative number. Every set of data was extracted and analyzed by STATA 14.0. The forest plot and bivariate boxplot were utilized to evaluate the heterogeneity. The funnel graph was used to test the publication bias. The SROC curve was draw via random effect model and HSROC model.</p><p>Result</p><p>17 sets of data and 3985 patients were included for the diagnostic meta-analysis. There was heterogeneity, which was partially from SCCa cut-off value. The pooled sensitivity was 0.70 and specificity was 0.63. AUC was 0.73. Eight articles provided the relative risk value of lymphatic metastasis when SCCa increased. The relative risk of lymph node metastasis increased ranging from 2.3–40 as with different SCCa cut off value.</p><p>Conclusion</p><p>The diagnostic value of SCCa for lymph nodal metastasis was medium and it was strongly related to lymph node status. Thus SCCa could assist imaging tests to detect lymph node metastasis. Besides, it was correlated with para-aortic lymph node metastasis.</p></div

The PRISMA flow diagram of literature screening.

<p>13 articles meet the inclusion and exclusion requirement eventually and were eligible for the meta-analysis.</p

The articles which provided the information about the relationship between the elevated SCCa and the risk of pelvic nodal metastasis.

<p>The articles which provided the information about the relationship between the elevated SCCa and the risk of pelvic nodal metastasis.</p

The results of quality evaluation of QUADAS.

<p>The results of quality evaluation of QUADAS.</p

The forest plot about the pooled sensitivity and specificity for the diagnostic value of SCCa on the lymph node metastasis was drawn.

<p>And the heterogeneity could be determined on the figure. The Irawn. And the heterogeneity could be determined on the figure. SCindicating there were heterogeneity between studies.(I²>50 suggested heterogeneity existed). The pooled sensitivity was 0.7 and specificity was 0.63, indicating a medium diagnostic power.</p

The bivariate boxplot about the heterogeneity was drawn.

<p>It demonstrated that 3 sets of data were out of the circles, which indicated there was heterogeneity between articles we included.</p