4 research outputs found

    Biochemical Parameters for Longitudinal Monitoring of Liver Function in Rat Models of Partial Hepatectomy Following Liver Injury

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    <div><p>Background</p><p>While evaluation of liver function in preclinical animal studies is commonly performed at selected time-points by invasive determination of the liver/body weight ratio and histological analyses, the validation of longitudinal measurement tools for monitoring liver function are of major interest.</p><p>Aims</p><p>To longitudinally evaluate serum cholinesterase (CHE) and total serum bilirubin (TSB) levels as non-invasive markers to determine injury- and partial hepatectomy (PHx)-induced alterations of liver function in rats.</p><p>Methods</p><p>Male and female Lewis rats were subjected to either methionine/choline deficient (MCD) diet or treatment with FOLFOX chemotherapy prior to PHx. Body weight and CHE/TSB levels are determined weekly. Following PHx and at the study end, histological analyses of liver tissue are performed.</p><p>Results</p><p>Following MCD diet, but not after FOLFOX chemotherapy treatment, results indicate gender-specific alterations in serum CHE levels and gender-independent alterations in TSB levels. Likewise, histological analyses of resected liver parts indicate significant liver injury following MCD-diet, but not following FOLFOX treatment. While TSB levels rapidly recover following MCD diet/FOLFOX treatment combined with a PHx, serum CHE levels are subject to significant model- and gender-specific differences, despite full histopathological recovery of liver tissue.</p><p>Conclusions</p><p>Longitudinal measurements of serum CHE levels and TSB levels in rats are highly complementary as non-invasive parameters for evaluation of liver injury and/or recovery.</p></div

    Recovery assessment following partial hepatectomy or MCD diet or chemotherapy in combination with PHx.

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    <p>(A/D) Evolution of body weight of male/female rats for each experimental group. Data are expressed in gram ± standard error. (B/E) Evolution of total serum bilirubin (TSB) of male/female rats for each experimental group. Data are expressed in milligram per deciliter ± standard error. (C/F) Evolution of serum cholinesterase (CHE) level of male/female rats for each experimental group. Data are expressed in units per liter ± standard error.</p

    Study design and liver weight following PHx or MCD diet or chemotherapy in combination with PHx.

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    <p>(A) Study design, for both male and female rats. (B/C) Mean liver weight for each experimental group at week 10 before PHx (left panels), at week 10 directly after PHx (middle panels) and at week 18 (end of the study, right panels); for male (B) and female (C). Data are expressed in gram ± standard error.</p

    Histological analysis of liver tissue following intraportal administration of BM-MSC after MCD diet and PHx in rat.

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    <p>Haematoxylin-eosin (H–E) staining (upper panel), Oil Red O staining (middle panel) and PAS/OX-1 staining (lower panel). (i) after 13 weeks of control diet (GROUP A), (ii) after 4 weeks of MCD diet, followed by PHx and control diet for 9 weeks (GROUP B), (iii) after 4 weeks of MCD diet, followed by PHx, BM-MSC administration and control diet for 9 weeks (GROUP C). Representative images were chosen out of 3 mice analysed per experimental group. For H-E staining, scale bars in the main image indicate 500 μm, while scale bars in the inset images indicate 50 μm. For Oil Red O and PAS/OX-1 staining, scale bars indicate 100 μm.</p
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