13 research outputs found
Temporal and regional risks for Zika virus introductions and transmission.
<p>(A) The temporal probabilities for Zika virus introductions via infected travelers and establishment of local transmssion are shown for 10 links, listed as origin-destination (rank). Relative risk profiles for temporal and regional Zika virus (B) exportations and (C) introductions were estimated using the network-level transmission probabilities (top 5 ranked regions shown for each). (D) Geographic variation in relative Zika virus importation risk for June, 2016. All Zika virus transmission data can be found in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006194#pntd.0006194.s001" target="_blank">S1 Data</a>. The maps were generated using open source shape files from Natural Earth (<a href="http://www.naturalearthdata.com/" target="_blank">http://www.naturalearthdata.com/</a>).</p
International air passenger travel used to construct the Zika virus epidemic model network.
<p>Passenger air travel from regions that reported Zika virus transmission was used to construct a network for potential international virus spread from the epidemic origin, Brazil (pink). The monthly travel volumes were normalized to fit between 0 and 1 and summed across the 18 month study period. The color gradient represents the relative arrival volumes at each destination. The weighted lines represent the travel volumes along each route with the thicker end pointing towards the destination. The travel routes connect region centroids, not specific air ports. We only used departing flights from Brazil (pink). The maps were generated using open source shape files from Natural Earth (<a href="http://www.naturalearthdata.com/" target="_blank">http://www.naturalearthdata.com/</a>).</p
Reported suspected and confirmed Zika cases in the Americas.
<p>Monthly distribution of reported (A) Zika cases and (B) Zika incidence rates for 15 countries and territories during the 2015-2016 epidemic (listed in descending order of incidence rates). (C) The number of countries and territories reporting local Zika cases per month. (D) The total number of Zika cases reported in the Americas per month and cumulatively during the epidemic.</p
Regional variability in socioeconomic and human population data in the Americas.
<p>Regional values for (A) gross domestic product (GDP) purchasing power parity rates per capita and (B) human population densities (people per sq. km of land area) used in the models. The boxes show zoomed in views of the Caribbean Islands. The maps were generated using open source shape files from Natural Earth (<a href="http://www.naturalearthdata.com/" target="_blank">http://www.naturalearthdata.com/</a>).</p
Sensitivity analyses accounting for delays in reporting Zika virus transmission.
<p>The model was re-run by shifting the reported Zika virus cases by three and six months for each region to account for delays in outbreak detection.</p
Inferring the risk factors behind the geographical spread and transmission of Zika in the Americas
<div><p>Background</p><p>An unprecedented Zika virus epidemic occurred in the Americas during 2015-2016. The size of the epidemic in conjunction with newly recognized health risks associated with the virus attracted significant attention across the research community. Our study complements several recent studies which have mapped epidemiological elements of Zika, by introducing a newly proposed methodology to simultaneously estimate the contribution of various risk factors for geographic spread resulting in local transmission and to compute the risk of spread (or re-introductions) between each pair of regions. The focus of our analysis is on the Americas, where the set of regions includes all countries, overseas territories, and the states of the US.</p><p>Methodology/Principal findings</p><p>We present a novel application of the Generalized Inverse Infection Model (GIIM). The GIIM model uses real observations from the outbreak and seeks to estimate the risk factors driving transmission. The observations are derived from the dates of reported local transmission of Zika virus in each region, the network structure is defined by the passenger air travel movements between all pairs of regions, and the risk factors considered include regional socioeconomic factors, vector habitat suitability, travel volumes, and epidemiological data. The GIIM relies on a multi-agent based optimization method to estimate the parameters, and utilizes a data driven stochastic-dynamic epidemic model for evaluation. As expected, we found that mosquito abundance, incidence rate at the origin region, and human population density are risk factors for Zika virus transmission and spread. Surprisingly, air passenger volume was less impactful, and the most significant factor was (a negative relationship with) the regional gross domestic product (GDP) per capita.</p><p>Conclusions/Significance</p><p>Our model generates country level exportation and importation risk profiles over the course of the epidemic and provides quantitative estimates for the likelihood of introduced Zika virus resulting in local transmission, between all origin-destination travel pairs in the Americas. Our findings indicate that local vector control, rather than travel restrictions, will be more effective at reducing the risks of Zika virus transmission and establishment. Moreover, the inverse relationship between Zika virus transmission and GDP suggests that Zika cases are more likely to occur in regions where people cannot afford to protect themselves from mosquitoes. The modeling framework is not specific for Zika virus, and could easily be employed for other vector-borne pathogens with sufficient epidemiological and entomological data.</p></div
Estimated risk factors for Zika virus transmission and spread.
<p>(A) The mean and standard deviation of the model coefficients estimated across 20 runs. The estimated coefficients represent the relative contributions of each risk factor. (B) The model performance mean and standard deviation values for each month of the observations period.</p
Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL
<div><p>Zika virus (ZIKV) is a mosquito-borne flavivirus of significant public health concern. In the summer of 2016, ZIKV was first detected in the contiguous United States. Here we present one of the first cases of a locally acquired ZIKV infection in a dengue-naïve individual. We collected blood from a female with a maculopapular rash at day (D) 5 and D7 post onset of symptoms (POS) and we continued weekly blood draws out to D148 POS. To establish the ontogeny of the immune response against ZIKV, lymphocytes and plasma were analyzed in a longitudinal fashion. The plasmablast response peaked at D7 POS (19.6% of CD19<sup>+</sup> B-cells) and was undetectable by D15 POS. ZIKV-specific IgM was present at D5 POS, peaked between D15 and D21 POS, and subsequently decreased. The ZIKV-specific IgG response, however, was not detected until D15 POS and continued to increase after that. Interestingly, even though the patient had never been infected with dengue virus (DENV), cross-reactive IgM and IgG binding against each of the four DENV serotypes could be detected. The highest plasma neutralization activity against ZIKV peaked between D15 and D21 POS, and even though DENV binding antibodies were present in the plasma of the patient, there was neither neutralization nor antibody dependent enhancement (ADE) of DENV. Interestingly, ADE against ZIKV arose at D48 POS and continued until the end of the study. CD4<sup>+</sup> and CD8<sup>+</sup> T-cells recognized ZIKV-NS2A and ZIKV-E, respectively. The tetramer positive CD8<sup>+</sup> T-cell response peaked at D21 POS with elevated levels persisting for months. In summary, this is the first study to establish the timing of the ontogeny of the immune response against ZIKV.</p></div
ZIKV induced maculopapular rash.
<p>Upon admission into the study photos of the patient’s rash were taken D3 POS. This rash was neither itchy, nor sensitive to the touch. (A) A close-up of the initiation point of the rash on the front of the torso near the midline. (B) The rash then spread from the torso to the neck and head.</p
Ontogeny of the plasmablast response of a primary ZIKV infection in a flavivirus-naïve individual.
<p>The blue box in each flow graph represents the frequency of Pbs from isolated PBMCs as a percentage of total CD19<sup>+</sup> B-Cells. The Pb frequency was significantly elevated at D5 POS when compared to a naïve uninfected individual as well as to convalescent phase PBMCs from Hu0015 (D148 POS).</p