Data_Sheet_1_Selective Feeding of a Mixotrophic Dinoflagellate (Lepidodinium sp.) in Response to Experimental Warming and Inorganic Nutrient Imbalance.docx

Mixotrophic protists are widely observed in the aquatic ecosystems, while how they respond to inorganic nutrient imbalance and ocean warming remains understudied. We conducted a series of experiments on a mixotrophic dinoflagellate Lepidodinium sp. isolated from subtropical coastal waters to investigate the combined effect of temperature and medium nitrate to phosphate ratio (N:P ratio) on the ingestion activities of mixotrophic protists. We found Lepidodinium sp. displayed selective feeding behaviour with a higher ingestion rate on high-N prey (N-rich Rhodomonas salina) when the ambient inorganic N:P ratio was equal to or below the Redfield ratio. The Chesson selectivity index α increased with increasing temperature, suggesting that warming exacerbated the selective feeding of Lepidodinium sp. Under inorganic nitrogen sufficient conditions (N:P ratio = 64), no selective feeding was observed at 25 and 28°C, while it occurs at 31°C, which also indicates that warming alters the feeding behaviour of Lepidodinium sp. In addition, our results revealed that the total ingestion rate of Lepidodinium sp. under the condition with normal inorganic nutrients (Redfield ratio) was significantly lower than that under nutrient-imbalanced conditions, which indicates that Lepidodinium sp. developed compensatory feeding to balance their cellular stoichiometry and satisfy their growth. Our study is the first attempt on revealing the selective feeding behaviours of mixotrophic protists on prey under different inorganic nutrient environments and rising temperatures, which will contribute to our understanding of the response of marine plankton food web to projected climate changes.</p

Direct Enol Ether Metalation–Negishi Coupling Strategy To Prepare α‑Heteroaryl Enol Ethers

A robust direct enol ether metalation–Negishi coupling using heteroaryl halides catalyzed by the palladium-Cy-DPEPhos system is reported. This method, which was demonstrated with a broad substrate scope, is a highly complementary method to the existing Heck coupling of synthesizing challenging α-heteroaryl-α-alkoxy alkenes

Additional file 1 of Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Additional file 1: Figure S1. GO classification and statistical results for all genes. The genes were summarized in biological process, cellular component and molecular function terms. A total of 28,662 genes were categorized

Additional file 7 of Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Additional file 7: Table S5. Primer pairs used for qRT-PCR verification of gene expression in rice

Additional file 4 of Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Additional file 4: Table S3. List of DEGs between Fen treatment and CK at 4 h

Additional file 5 of Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Additional file 5: Table S4. List of DEGs between Fen treatment and CK at 24 h

Additional file 6 of Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Additional file 6: Figure S2. GO classification and statistical results for DEGs at 4 h (A) and 24 h (B) of treatment. The genes were summarized in biological process, cellular component and molecular function terms. A total of 168 differentially expressed genes at 4 h of treatment and 68 differentially expressed genes at 24 h of treatment were annotated

Additional file 3 of Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Additional file 3: Table S2. Read FPKM per gene

Degradation dynamics of dimethoate in Bok choy by TiO₂/Ce.

<p>The residue change of dimethoate in Bok choy (<b>A</b>: original data; <b>B</b>: log transferred data). C₀ and C represent the initial and reacting (time = t) residues of dimethoate in Bok choy, respectively. The C₀ of dimethoate residual concentrations of 600 g a.i. /ha for time zero was 15.859 mg/kg.</p

Bond length on main atoms in dimethoate molecule at the RHF/STO-3 level.

<p>Bond length on main atoms in dimethoate molecule at the RHF/STO-3 level.</p