Additional file 1 of Choosing panels of genomics assays using submodular optimization

Additional figures and text. (PDF 606 kb

Additional file 2 of Choosing panels of genomics assays using submodular optimization

List of all assays used. File is in gzipped, tab-delimited format. Columns correspond to: (1) assay type, (2) cell type, (3) file name of file on original server, and (4) URL of server that the file was downloaded from. (TAB 300 kb

Cascade C(sp³)–S Bond Cleavage and Imidoyl C–S Formation: Radical Cyclization of 2‑Isocyanoaryl Thioethers toward 2‑Substituted Benzothiazoles

A cascade radical cyclization of 2-isocyanoaryl thioethers with <i>H</i>-phosphorus oxides, organoboronic acids, or alkyl radical precursors has been efficiently developed, providing a novel and highly efficient methodology to structurally diverse C2-substituted benzothiazole derivatives with broad functional group tolerance and good yields. This cascade radical process achieves the first cycloaddition of an imidoyl radical from isocyanide to sulfur atom, rending C­(sp²)–S bond formation

Mechanical Activation of Platinum–Acetylide Complex for Olefin Hydrosilylation

Harnessing mechanical forces to activate latent catalysts has emerged as a novel approach to control the catalytic reactions in organic syntheses and polymerization processes. However, using polymer mechanochemistry to activate platinum-based catalysts, a class of important organometallic catalysts in industry, has not been demonstrated so far. Here we show that the platinum–acetylide complex is mechanoresponsive and can be incorporated into a polymer backbone to form a new mechanophore. The mechanically induced chain scission was demonstrated to be able to release catalytically active platinum species which could catalyze the olefin hydrosilylation process. Various control experiments were conducted to confirm that the chain scission and catalytic reaction were originated from the ultrasound-induced dissociation of platinum–acetylide complex. This work further exemplifies the utilization of organometallic complexes in design and synthesis of latent catalysts for mechanocatalysis and development of self-healing materials based on silicone polymers

Indoor particle bounce reductions rates using mineral-oil-spread agar plate in replace of agar plate; the particle concentration levels were monitored using an optical particle counter; data points represent averages and standard deviations of 20 min continuous data; a negative value suggests an increase in particle bounce.

<p>Indoor particle bounce reductions rates using mineral-oil-spread agar plate in replace of agar plate; the particle concentration levels were monitored using an optical particle counter; data points represent averages and standard deviations of 20 min continuous data; a negative value suggests an increase in particle bounce.</p

The illustration of use of mineral-oil-spread agar plate in improving the culturable bioaerosol sampling by the Andersen type sampler.

<p>The illustration of use of mineral-oil-spread agar plate in improving the culturable bioaerosol sampling by the Andersen type sampler.</p

Biological collection efficiencies of BioStage impactor together with agar plate and mineral-oil-spread agar plate in sampling the total fungal aerosols under different sampling times (5, 10 and 20 min) in an indoor environment; 100 µL mineral oil was evenly spread onto entire agar plate; 30 mL agar was used to fill the agar plate; data points represent averages and standard deviations of three independent sampling experiments;***** indicates a statistically significant difference.

<p>Biological collection efficiencies of BioStage impactor together with agar plate and mineral-oil-spread agar plate in sampling the total fungal aerosols under different sampling times (5, 10 and 20 min) in an indoor environment; 100 µL mineral oil was evenly spread onto entire agar plate; 30 mL agar was used to fill the agar plate; data points represent averages and standard deviations of three independent sampling experiments;***** indicates a statistically significant difference.</p

Size specific biological collection efficiencies of Andersen six-stage impactor together with agar plate and mineral-oil-spread agar plate in sampling fungal aerosols at a sampling time of 10 min in an indoor environment; 100 µL mineral oil was evenly spread onto entire agar plate; 30 mL agar was used to fill the agar plate; data points represent averages and standard deviations of three independent sampling experiments;***** indicates a statistically significant difference.

<p>Size specific biological collection efficiencies of Andersen six-stage impactor together with agar plate and mineral-oil-spread agar plate in sampling fungal aerosols at a sampling time of 10 min in an indoor environment; 100 µL mineral oil was evenly spread onto entire agar plate; 30 mL agar was used to fill the agar plate; data points represent averages and standard deviations of three independent sampling experiments;***** indicates a statistically significant difference.</p

Isolation and purification of polysaccharides.

<p>(A) Ion exchange chromatogram of TPPPS on DEAE-cellulose column, eluted with distilled water, 0.01, 0.03, 0.05, 0.1, and 0.3 M NaCl. T1: TPPPS1; T2: TPPPS2; T3: TPPPS3. (B, C, and D) Gel filtration chromatogram of TPPPS1, TPPPS2, and TPPPS3, respectively, on Sephadex G-200 column eluted with 0.1 M NaCl.</p

Effects of TPPPS1-3 and TPPPS on antiviral activity <i>in vitro</i>.

<p>(A) DF1 cells cultured in 24-well plates were infected with 10 μL/well (TCID₅₀ of 10^3.75/0.1 mL) ALV-B. After 9 d of culture in MM containing different concentrations of TPPPS or TPPPS1-3, viral titers in the supernatant were directly determined by TCID₅₀ assays on DF1 cells. PBS treatment wells served as the control. All values shown are presented as means ± SD from three independent experiments. An asterisk indicates that the value of the corresponding group was significantly different from that of the control group (<i>P</i> < 0.05, ANOVA). (B) DF1 cells infected with ALV-B were treated with 50 μg/mL of TPPPS3 at different viral infection periods, and viral titers in the supernatant were directly determined by TCID₅₀ assays on DF1 cells. Ad is defined as the “adsorption” period; AA is defined as the period “after adsorption”; Al is the period wherein the polysaccharide was always present. PBS treatment wells served as the control. All values shown are presented as means ± SD from three independent experiments. Different superscripts indicate a significant difference (<i>P</i> < 0.05).</p