ANTIDIABETIC AND ANTIDYSLIPIDEMIC PROPERTIES OF GOA-111, A MIXTURE OF GYMNEMA SYLVESTRAE, OCIMUM SANCTUM AND AZADIRACHTA INDICA EXTRACT IN THE RATIO OF 1:1:1 STUDIED IN HIGH FAT DIET FED- LOW DOSE STREPTOZOTOCIN INDUCED EXPERIMENTAL TYPE 2 DIABETES IN RAT

Diabetes mellitus emerges from multiple biochemical and cellular impairments, including decreased insulin secretion from the pancreatic β-cells and impaired insulin action in peripheral tissues. The present study was systematically carried out to evaluate antidiabetic and  antidyslipidemic properties of GOA-111,a herbal extract containing a mixture of Gymnema sylvestrae, Ocimum sanctum leaves and seed kernel of Azadirachta indica  in the ratio of 1:1:1 in ameliorating both the primary and secondary complications of type 2 diabetes mellitus in high fat diet fed low dose streptozotocin induced diabetic rats Experimental type 2 diabetes was induced with a low dose streptozotocin in rats  fed on a high fat diet. Diabetic rats were treated with three different doses GOA 111  (150,300 and 450 mg/Kg b.wt/rat/day)   for 30 days. The toxicological parameters such as AST, ALT and ALP were assayed. Biochemical parameters such as fasting blood glucose, glycosylated hemoglobin, insulin, insulin resistance and lipid profile were measured. Oral treatment with GOA 111 significantly decreased the elevated levels of fasting glucose, glycosylated hemoglobin, AST, ALT and ALP. The insulin level was improved in insulin resistant diabetic rats.  GOA 111 also normalized the lipid profile. Though the  results showed a dose dependent impact on the parameters, a dose of  300mg/Kg B/W/rat/day GOA 111 exerts maximum potential anti-hyperglycemic and antidyslipidemic effects in HFD/STZ-induced type 2 diabetic rats. Key words: GOA 111; High fat diet; Streptozotocin; antidiabetic; antidyslipidemi

ANTIDIABETIC AND ANTIDYSLIPIDEMIC PROPERTIES OF GOA-111, A MIXTURE OF GYMNEMA SYLVESTRAE, OCIMUM SANCTUM AND AZADIRACHTA INDICA EXTRACT IN THE RATIO OF 1:1:1 STUDIED IN HIGH FAT DIET FED- LOW DOSE STREPTOZOTOCIN INDUCED EXPERIMENTAL TYPE 2 DIABETES IN RAT

Diabetes mellitus emerges from multiple biochemical and cellular impairments, including decreased insulin secretion from the pancreatic β-cells and impaired insulin action in peripheral tissues. The present study was systematically carried out to evaluate antidiabetic and  antidyslipidemic properties of GOA-111,a herbal extract containing a mixture of Gymnema sylvestrae, Ocimum sanctum leaves and seed kernel of Azadirachta indica  in the ratio of 1:1:1 in ameliorating both the primary and secondary complications of type 2 diabetes mellitus in high fat diet fed low dose streptozotocin induced diabetic rats&#x0D; Experimental type 2 diabetes was induced with a low dose streptozotocin in rats  fed on a high fat diet. Diabetic rats were treated with three different doses GOA 111  (150,300 and 450 mg/Kg b.wt/rat/day)   for 30 days. The toxicological parameters such as AST, ALT and ALP were assayed. Biochemical parameters such as fasting blood glucose, glycosylated hemoglobin, insulin, insulin resistance and lipid profile were measured.&#x0D; Oral treatment with GOA 111 significantly decreased the elevated levels of fasting glucose, glycosylated hemoglobin, AST, ALT and ALP. The insulin level was improved in insulin resistant diabetic rats.  GOA 111 also normalized the lipid profile.&#x0D; Though the  results showed a dose dependent impact on the parameters, a dose of  300mg/Kg B/W/rat/day GOA 111 exerts maximum potential anti-hyperglycemic and antidyslipidemic effects in HFD/STZ-induced type 2 diabetic rats.&#x0D; Key words: GOA 111; High fat diet; Streptozotocin; antidiabetic; antidyslipidemic</jats:p

Molecular Docking Analysis of Compounds from Polyherbal Powder Against the Proteins Involved in Diabetes Mellitus

Objective: The objective of the present work is to perfom in silico docking analysis against the molecular targets involved in diabetes mellitus using active compounds from Polyherbal powder. Materials and methods: Polyherbal powder is prepared using Gymnema sylvestre, Ocimum sanctum and Azadirachta indica. Three compounds from the polyherbal powder (identified using HR-LCMS) were used for the docking against the targets (α-glucosidase, α amylase, peroxisome proliferator activated receptor gamma). Docking was performed using Autodock 4.2 software. Pymol was used for the visualization of the docking result files. Results: Binding energy of Usnic acid (-4.25 kcal/mol for 1PRG, -6.69 kcal/mol for 3WY1, -5.72 kcal/mol for 4GQQ) was low as that of standard drug Metformin (-5.07 kcal/mol for 1PRG, -6.3 kcal/mol for 3WY1, -4.02 kcal/mol for 4GQQ). Conclusion: The polyherbal powder containing the lead compounds can be used for the treatment of diabetes mellitus.</jats:p

03.MDI

Abstract In the present study, an attempt was made to compare the statistical tools used for analysing the data of repeated dose toxicity studies with rodents conducted in 45 countries, with that of Japan. The study revealed that there was no congruence among the countries in the use of statistical tools for analysing the data obtained from the above studies. For example, to analyse the data obtained from repeated dose toxicity studies with rodents, Scheffé&apos;s multiple range and Dunnett type (joint type Dunnett) tests are commonly used in Japan, but in other countries use of these statistical tools is not so common. However, statistical techniques used for testing the above data for homogeneity of variance and inter-group comparisons do not differ much between Japan and other countries. In Japan, the data are generally not tested for normality and the same is true with the most of the countries investigated. In the present investigation, out of 127 studies examined, data of only 6 studies were analysed for both homogeneity of variance and normal distribution. For examining homogeneity of variance, we propose Levene&apos;s test, since the commonly used Bartlett&apos;s test may show heterogeneity in variance in all the groups, if a slight heterogeneity in variance is seen any one of the groups. We suggest the data may be examined for both homogeneity of variance and normal distribution. For the data of the groups that do not show heterogeneity of variance, to find the significant difference among the groups, we recommend Dunnett&apos;s test, and for those show heterogeneity of variance, we recommend Steel&apos;s test

03.MDI

Abstract In the present study, an attempt was made to compare the statistical tools used for analysing the data of repeated dose toxicity studies with rodents conducted in 45 countries, with that of Japan. The study revealed that there was no congruence among the countries in the use of statistical tools for analysing the data obtained from the above studies. For example, to analyse the data obtained from repeated dose toxicity studies with rodents, Scheffé&apos;s multiple range and Dunnett type (joint type Dunnett) tests are commonly used in Japan, but in other countries use of these statistical tools is not so common. However, statistical techniques used for testing the above data for homogeneity of variance and inter-group comparisons do not differ much between Japan and other countries. In Japan, the data are generally not tested for normality and the same is true with the most of the countries investigated. In the present investigation, out of 127 studies examined, data of only 6 studies were analysed for both homogeneity of variance and normal distribution. For examining homogeneity of variance, we propose Levene&apos;s test, since the commonly used Bartlett&apos;s test may show heterogeneity in variance in all the groups, if a slight heterogeneity in variance is seen any one of the groups. We suggest the data may be examined for both homogeneity of variance and normal distribution. For the data of the groups that do not show heterogeneity of variance, to find the significant difference among the groups, we recommend Dunnett&apos;s test, and for those show heterogeneity of variance, we recommend Steel&apos;s test

Hypolipidemic effect of <i>Coriandrum sativum </i>L. in<b> </b>triton-induced hyperlipidemic rats

909-912In the biphasic model of triton-induced hyperlipidemia, C. sativum at a dose of 1g/kg body weight reduced cholesterol and triglycerides levels in both synthesis and excretory phases in rats, and the results were comparable with that of Liponil, a commercially available herbal hypolipidemic drug. The results suggest that coriander decreases the uptake and enhances the breakdown of lipids. From the study it can be assumed that coriander has the potential to be popularized as a household herbal remedy with preventive and curative effect against hyperlipidemia