data crossinfection experiment Antagonistic coevolution accelerates the evolution of reproductive isolation in Tribolium castaneum

data crossinfection experiment Antagonistic coevolution accelerates the evolution of reproductive isolation in Tribolium castaneu

data main experiment Antagonistic coevolution accelerates the evolution of reproductive isolation in Tribolium castaneum

data main experiment Antagonistic coevolution accelerates the evolution of reproductive isolation in Tribolium castaneu

Distribution of induced virulence (%) from May to October 2010.

<p>Shown are median virulence values (%) per sampling month, with an interquartile box and a 5 to 95% range. Outliers are marked by black dots.</p

Lag analyses for the correlation between diversity (Shannon Index) and mean temperature integrated over 28 days prior to the sampling event.

<p>Day 0 corresponds to the temperature measured at the sampling day representing an immediate response, while day 28 uses the mean temperature of the previous month representing a slower response to temperature shifts. Significant correlations between diversity and temperature are depicted by black triangles.</p

Phylogenetic relationships of <i>Vibrio</i> isolates sampled from oyster hemolymph.

<p>Maximum likelihood method (GTR+G+I substitution model) using concatenated sequences of three genes (16S rRNA, <i>GyrB</i>, <i>PyrH</i>) (2560 bp). Bootstrap percentages above 80% are represented by grey dots at the parent nodes. Virulence of each strain is depicted by the grey bars on the outer ring (a value of 100 corresponds to 100% mortality during inoculation experiments). Assignment to the different clades (see legend) based on 99% sequence similarity by BLASTN analysis is depicted in color on the inner ring.</p

Predicted probability to encounter highly pathogenic strains (i.e. induced mortality >25%) for each sampling month.

<p>Predicted probability to encounter highly pathogenic strains (i.e. induced mortality >25%) for each sampling month.</p

Temporal variation in the amount of <i>Vibrio</i> bacteria in oyster hemolymph (HL).

<p>Quantification of total <i>Vibrio</i> spp. (open circles and triangles) isolated from oyster hemolymph stemming from the four transplant groups (origin_site), i.e. DB_DB, DB_OW, OW_DB, OW_OW. Blue lines represent oysters assayed in site OW and black lines represent oysters assayed in site DB. Solid lines show oyster origin DB, while dashed lines show oyster origin OW. Water temperature is shown on the secondary y-axis (full circles, dotted line). The area shaded in grey marks the spawning period. Different letters indicate significant differences according to Tukey HSD post hoc test. Pi charts show relative proportion of <i>Vibrio</i> isolates by phylogenetic association based on 99% similarity by BLASTN analysis in each sampling month. Color codes correspond to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094256#pone-0094256-g002" target="_blank">Figure 2</a>.</p

Number of OTUs and richness estimators (Chao1 and Shannon-Wiener Index) for each sampling month (May–Oct) as well as at both sampling sites (Dietrichsenbank and Oddewatt) on the basis of 99% identity cut-off.

<p>Number of OTUs and richness estimators (Chao1 and Shannon-Wiener Index) for each sampling month (May–Oct) as well as at both sampling sites (Dietrichsenbank and Oddewatt) on the basis of 99% identity cut-off.</p

Shama et al_Ischnura ms data

microsatellite genotype

Sample sizes of introduced <i>Mytilicola</i> species in different sub-categories.

<p>Sample sizes of introduced <i>Mytilicola</i> species in different sub-categories.</p