6 research outputs found
Transcriptomic analysis of <i>Crassostrea sikamea</i> × <i>Crassostrea angulata</i> hybrids in response to low salinity stress
<div><p>Hybrid oysters often show heterosis in growth rate, weight, survival and adaptability to extremes of salinity. Oysters have also been used as model organisms to study the evolution of host-defense system. To gain comprehensive knowledge about various physiological processes in hybrid oysters under low salinity stress, we performed transcriptomic analysis of gill tissue of <i>Crassostrea sikamea</i> ♀ × <i>Crassostrea angulata</i>♂ hybrid using the deep-sequencing platform Illumina HiSeq. We exploited the high-throughput technique to delineate differentially expressed genes (DEGs) in oysters maintained in hypotonic conditions. A total of 199,391 high quality unigenes, with average length of 644 bp, were generated. Of these 35 and 31 genes showed up- and down-regulation, respectively. Functional categorization and pathway analysis of these DEGs revealed enrichment for immune mechanism, apoptosis, energy metabolism and osmoregulation under low salinity stress. The expression patterns of 41 DEGs in hybrids and their parental species were further analyzed by quantitative real-time PCR (qRT-PCR). This study will serve as a platform for subsequent gene expression analysis regarding environmental stress. Our findings will also provide valuable information about gene expression to better understand the immune mechanism, apoptosis, energy metabolism and osmoregulation in hybrid oysters under low salinity stress.</p></div
Length distribution of unigenes and transcripts.
<p>Length distribution of unigenes and transcripts.</p
Primer sequences for amplification of target and reference genes in three different species.
<p>Primer sequences for amplification of target and reference genes in three different species.</p
Cluster analysis of DEGs in SS, SA and AA oysters based on their relative expression level as determined by qRT-PCR.
<p>Blue represents lower expression, and red represents higher expression. Each column represents a comparison between low salinity samples and control samples for each species. Each row represents a gene. The yellow square represents immune- or apoptosis-related gene, the green square represents genes involved in osmotic regulation, and the purple square represents genes involved in energy regulation. The 41genes were classified into five groups.</p