Table_1_Changes in lung cancer-related serum tumor markers in patients with chronic kidney disease and determination of upper reference limit.docx

AimsTo investigate the changes in lung cancer-related serum tumor markers in patients with chronic kidney disease (CKD) and determine the upper reference limit for patients with different stages.MethodsIncluded inpatients diagnosed with CKD who did not receive dialysis temporarily in our hospital from March to September 2020. Changes in serum CA125, HE4, CYFRA21-1, SCCA, NSE and ProGRP in CKD patients were analyzed. The non-parametric method was used to estimate the upper reference limit of the above indicators in patients with CKD stages 2-5.ResultsThe serum levels of HE4, CYFRA21-1, SCCA, and ProGRP in the CKD group were significantly higher than those in the healthy control group; CA125 and NSE levels were not statistically different. The false positives of SCC, CYFRA21-1, ProGRP, and HE4 increased significantly with the CKD stage. Still, NSE and CA125 did not show a significant increasing trend. Both HE4 and ProGRP have independent upper reference limits from CKD2 to CKD5 stage, namely 220.8 pmol/l and 101.4 pg/ml in the CKD2 stage, 496.7 pmol/l and 168.63 pg/ml in CKD3 stage, 4592.4 pmol/l and 272.8 pmol/l for CKD4 stage, CKD5 stage was 4778.2 pmol/l and 491.6 pmol/l.ConclusionThis study preliminarily determined the upper reference limits of Lung cancer-related tumor markers in patients with different CKD stages and provided laboratory support for the rational use and interpretation of Lung cancer-related tumor markers in special populations.</p

Image_1_Unraveling the intricacies of glioblastoma progression and recurrence: insights into the role of NFYB and oxidative phosphorylation at the single-cell level.tif

BackgroundGlioblastoma (GBM), with its high recurrence and mortality rates, makes it the deadliest neurological malignancy. Oxidative phosphorylation is a highly active cellular pathway in GBM, and NFYB is a tumor-associated transcription factor. Both are related to mitochondrial function, but studies on their relationship with GBM at the single-cell level are still scarce.MethodsWe re-analyzed the single-cell profiles of GBM from patients with different subtypes by single-cell transcriptomic analysis and further subdivided the large population of Glioma cells into different subpopulations, explored the interrelationships and active pathways among cell stages and clinical subtypes of the populations, and investigated the relationship between the transcription factor NFYB of the key subpopulations and GBM, searching for the prognostic genes of GBM related to NFYB, and verified by experiments.ResultsGlioma cells and their C5 subpopulation had the highest percentage of G2M staging and rGBM, which we hypothesized might be related to the higher dividing and proliferating ability of both Glioma and C5 subpopulations. Oxidative phosphorylation pathway activity is elevated in both the Glioma and C5 subgroup, and NFYB is a key transcription factor for the C5 subgroup, suggesting its possible involvement in GBM proliferation and recurrence, and its close association with mitochondrial function. We also identified 13 prognostic genes associated with NFYB, of which MEM60 may cause GBM patients to have a poor prognosis by promoting GBM proliferation and drug resistance. Knockdown of the NFYB was found to contribute to the inhibition of proliferation, invasion, and migration of GBM cells.ConclusionThese findings help to elucidate the key mechanisms of mitochondrial function in GBM progression and recurrence, and to establish a new prognostic model and therapeutic target based on NFYB.</p

IL-28B SNPs in LC and HCC caused by HBV, group of CHB and health control.

<p>Values are shown as number (frequency), Data is analyzed by chi-squre analyses.</p>*<p>refer to P<0.05.</p

Demographic characteristics of LC and HCC patients caused by hepatitis B.

<p>Note:values are shown as mean ± SD or median (range). Data were analyzed by chi-squre analyses test and Mann–Whitney U test.</p>*<p>:P<0.05.</p

IL-28B SNPs in LC and HCC caused by HBV (verification by independent sample).

<p>Values are shown as number (frequency), Data is analyzed by chi-squre analyses.</p>*<p>refer to P<0.05.</p

There are three groups of curves in the figure, red ones and green ones indicate homozygote, red curves on the right side indicates G or C whose Tm is high, green curves on the left side indicates A or T whose Tm is low, and the blue curves in the middle indicates heterozygote.

<p>The genotype of subset was defined according to known genotypes of controls which were determinate by gene sequencing.</p

Multiplexed Detection of Attomoles of Nucleic Acids Using Fluorescent Nanoparticle Counting Platform

The sensitive multiplexed detection of nucleic acids in a single sample by a simple manner is of pivotal importance for the diagnosis and therapy of human diseases. Herein, we constructed an automatic fluorescent nanoparticle (FNP) counting platform with a common fluorescence microscopic imaging setup for nonamplification multiplexed detection of attomoles of nucleic acids. Taking the advantages of the highly bright, multicolor emitting FNPs and magnetic separation, the platform enables sensitive multiplexed detection without the need for extra fluorescent labels. Quantification for multiplex DNAs, multiplex microRNAs (miRNA), as well as a DNA and miRNA mixture was achieved with a similar dynamic range, a limit of detection down to 5 amol (5 μL detection volume), and a 81–115% spike recovery from different biological sample matrices. In particular, the sensitivity for multiplex miRNA is by far among the highest without using amplification or the lock nucleic acid hybridization enhancement strategy. Results regarding miRNA-141 from four different cell lines were agreeable with those of the quantitative reverse transcription polymerase chain reaction. Simultaneous detection of miRNA-141 and miRNA-21 in four different cell lines yielded consistent results with publications, indicating the potential for monitoring multiplex miRNA expression associated with the collaborative regulation of important cellular events. This work expands the rule set of multiplex nucleic acid detection strategies and shows promising potential application in clinical diagnosis

Logistic regression of clinical characteristics and SNPs with HCC occurrence.

<p>Note: Logistic regression models were used for calculating odds ratios (95% confidence interval) and corresponding <i>P</i>-values for each SNP site and HCC occurrence. Age (continuous value) and sex (male  =  0, female  =  1) were adjusted by inclusion in logistic analysis as covariates. (*p<0.05).</p

The proportion of IgAN patients who were grouped as co-dominant model and recessive model for different age groups.

<p>Comparisons between groups were made by the χ2 test as appropriate.</p><p>The proportion of IgAN patients who were grouped as co-dominant model and recessive model for different age groups.</p

Genotype and allele distributions of rs2910164 C>G.

<p>Comparisons between groups were made by the χ2 test as appropriate.</p><p>*<i>P</i>-value of Hardy-Weinberg equilibrium; NS: no significance.</p><p>Genotype and allele distributions of rs2910164 C>G.</p