Scheme of one possible reaction in permanganate oxidation of polystyrene and its subsequent reaction with DBAP.

<p>Scheme of one possible reaction in permanganate oxidation of polystyrene and its subsequent reaction with DBAP.</p

Absorbance of anti-DBP antibody bound to hapten-coated polystyrene wells.

<p>Absorbance at 492 nm under different concentrations of EDC and conjugation temperatures. Each data represents the mean of ±SD of three replicates (three plates on the same day).</p

Cross-reactivity of some compounds structurally related to DBP by hapten coated icELISA.

<p><i>^a</i>Each data represents the mean of ±SD of three replicates (three plates on the same day).</p

Recovery of DBP spiked into water samples.

<p><i>^a</i>Each data represents the mean of ±SD of three replicates (the same sample run on one plate on three different days).</p

Correlation of ELISA and GC-MS analysis of certain household items.

<p>Each point represents the mean of three replicates of one sample.</p

Representative icELISA standard curves for DBP.

<p>Each point represents the mean of ±SD of three replicates (one plate on three different days). The standard curve was calculated according to the 4-parameter equation <i>y</i> = (<i>A</i>−<i>D</i>)/[1+(<i>x</i>/<i>C</i>)<i>^B</i>)]+<i>D</i> with the following values: for hapten coated icELISA, <i>A</i> = 0.956, <i>B</i> = 0.705, <i>C</i> = 14.6 ng/mL, <i>D</i> = 0.027, R² = 0.9985; for conjugate coated icELISA, <i>A</i> = 0.892, <i>B</i> = 0.994, <i>C</i> = 106 ng/mL, <i>D</i> = 0.0094, R² = 0.9976.</p

Influence of organic solvents in assay buffer on parameters of icELISA.

<p>Each data represents the mean of ±SD of three replicates (three plates on the same day).</p

Titers and sensitivity of antisera of immunized mice.

<p><i>^a</i>The coating concentration was 1 µg/mL.</p><p><i>^b</i>The last dilution of serum that gave an absorbance at 492 nm of >1.0 was judged as the titer.</p><p><i>^c</i>IC₅₀ represents the mean of 3 wells replicates.</p

Effects of pH (A), ionic strength (B) and detergent (C) in assay buffer and blocking reagents (D) on icELISA for DBP.

<p>Each point represents the mean of ±SD of three replicates (three plates on the same day).</p

UV absorbance spectra of DBAP, OVA, DBAP-OVA (A) and DBCP, OVA, DBCP-OVA (B).

<p>All the concentrations of the tested substances were 0.5 mg/mL.</p