Morfologi, Fisiologi, Preservasi Sel Sperma Ikan Betok, Anabas Testudineus Bloch 1792 Dan Ketahanannya Terhadap Kejut Listrik [Morphology, Physiology, Preservation of Climbing Perch Anabas Testudineus Bloch 1792 Sperm and the Endurance to Electric Shock]

Kualitas sperma yang baik dan dalam keadaan tak aktif selama proses elektroporasi sangat menentukan keberhasilan transgenesis. Penelitian ini bertujuan untuk mengeksplorasi karakter morfologis dan fisiologis sel sperma, serta menge-valuasi efek voltase dan jumlah kejut listrik berbeda terhadap motilitas dan viabilitas sperma ikan betok. Pengamatan karakter morfologi dilakukan pada mikroskop dengan pembesaran 400x, karakter fisiologi dengan berbagai parameter yakni tingkat osmolaritas, volume, pH, kepadatan dan total sperma. Hasil penelitian menunjukkan bahwa sperma ikan betok terdiri atas tiga bagian, yakni kepala yang berbentuk oval, bagian tengah, dan ekor sperma. Viabilitas sperma ikan betok dalam air adalah selama 116±34 detik. Induksi ovaprim dapat meningkatkan volume sperma, pH, kepadatan dan total sperma (p<0,05). Tingkat osmolaritas tubuh dan testis ikan betok (390-405 mOsmol kg-1). Penyimpanan sperma yang diencerkan dengan larutan NaCl 1,3% hanya dapat bertahan maksimum selama 60 menit pada suhu ruang (26°C) dan suhu dingin (4°C), sedangkan tanpa pengenceran dapat bertahan selama 360 menit. Derajat pembuahan dan kelangsungan hidup larva hingga 10 hari pascatetas menggunakan sperma dari testes cacah sama dengan pemijahan semi alami. Elektroporasi sperma ikan betok dengan pengencer larutan NaCl 1,3% berhasil dilakukan menggunakan lama kejut 0,5 milidetik, dan jeda antar kejut 0,1 detik. Peningkatan voltase dan jumlah kejut yang diberikan saling berinter-aksi menurunkan motilitas dan viabilitas sperma (p<0,05). Metode kejut listrik tersebut berpotensi digunakan untuk menghasilkan ikan betok transgenik dengan metode elektroporasi

Efeito do tamanho de gotas e formulações de iscas tóxicas sobre a mortalidade de adultos da mosca-das-frutas sul-americana

Neste trabalho foi avaliado o efeito de diferentes tamanhos de gota de formulações de iscas tóxicas sobre a mortalidade de adultos de A. fraterculus em laboratório

Variabilidade espacial e temporal de Anastrepha fraterculus na cultura da videira.

O comportamento dos insetos ao longo do tempo e do espaço fornece informações que permitem identificar sua dispersão permitindo direcionar estratégias de manejo. Neste trabalho, foi avaliada a distribuição espaçotemporal de adultos da mosca-das-frutas-sulamericana, Anastrepha fraterculus, em área de produção comercial de uva da cv. ?Moscato ? (Vitis vinífera L.) plantada em no espaçamento de 2,5m x 1,5m

Collaborative environments in RD&I institutions of the brazilian agricultural sector.

Research, development and technological innovation (RD&I) companies promote inter- and intra-organizational interactions based on the formation of research nets. This arrangement prioritizes the use of competencies of both people and institutions, stimulating the generation of new knowledge and competencies, and consequently technological innovation. The Brazilian Agricultural Research Corporation – Embrapa, responsible for knowledge generation and technological innovation in the Brazilian agricultural sector, has put a lot of effort into the establishment of a collaborative environment which allows the exchange of information, knowledge and experience among researchers and promote new knowledge, competencies and technological innovation. The present paper discusses conceptual bases and presents a model of collaborative environment established by the above-mentioned corporation in order to favor the learning and the production of innovative solutions in agricultural research nets

Feminisasi Sidat, Anguilla Bicolor Bicolor Mc Clelland, 1844 Melalui Penyuntikan Hormon Estradiol Dan Metil Testosteron Yang Dikombinasi Dengan HCG Dan Anti Dopamin [Feminization of Indonesian Short-finned Eel (Anguilla Bicolor Bicolor Mc Clelland, 1844) Through Injection of Estradiol and Methyl Testosterone at the Combination with HCG and Anti Dopamin]

Penelitian ini bertujuan untuk menjelaskan pengaruh estradiol (E2) dan metil testosteron (MT) yang dikombinasikan dengan hCG dan anti dopamin (AD) terhadap persentase jenis kelamin betina dan kualitas perkembangan ovari sidat (Anguilla bicolor bicolor). Sidat dengan bobot 200±15g pada faseyellow diinjeksi dengan larutan estradiol 3 mg mL-1 + hCG, 2 mg mL-1 + AD 10 mg mL-1 (hEA), MT 3 mg mL-1 + hCG 2 mg mL-1 + AD 10 mg mL-1 (hTA), estradiol 3 mg mL-1 + AD 10 mg mL-1 (EA), MT 3 mg mL-1 + AD 10 mg mL-1 (TA), AD 10 mg mL-1 (A), dan kontrol 9 mg mL-1 NaCl (F). Frekuensi injeksi sebanyak enam kali dengan periode dua minggu sekali. Pemberian hormon kombinasi dengan perlakuan hEA dan hTA ternyata efektif meningkatkan persentase jenis kelamin betina berturut-turut sebesar 72% dan 66% setelah enam minggu. Perkembangan gonad betina terbaik ditemukan pada perlakuan hTA yaitu mencapai fase vitellogenesis akhir dengan nilai GSI 4,80

[Hormone Profile of FSH, LH and Estradiol with Glucose Blood Level of Indonesian Short-finned Eel (Anguilla Bicolor Bicolor Mc Clelland, 1844) Stimulated by HCG, MT, E2 and Dopamine Inhibitory

This study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range. AbstrakThis study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range

Pematangan Gonad Ikan Sidat Betina (Anguilla Bicolor Bicolor) melalui Induksi Ekstrak Hipofisis

Tujuan penelitian ini adalah untuk menjelaskan pengaruh pemberian ekstrak hipofisis ikan mas terhadap pematangan gonad ikan sidat tropis betina (Anguilla bicolor bicolor). Ikan sidat dengan bobot 600 gram telah digunakan sebagai materi biologis. Ekstrak hipofisis ikan mas dengan tingkat 0, ¼ dan ½ dosis dan tiga tipe frekuensi penyuntikan yakni setiap 1, 2 dan 3 minggu dijadikan sebagai perlakuan. Parameter yang diamati adalah Gonado somatic index (GSI), Hepatosomatic index (HSI), diameter folikel, kadar lemak dalam folikel, serta kadar glikokonjugat dalam folikel dan hati. Hasil percobaan menunjukkan bahwa penyuntikan ekstrak hipofisis ikan mas dengan ½ dosis yang dilakukan setiap minggu selama 10 minggu memberikan pengaruh yang terbaik

BRS Harmonia - triticale cultivar.

The triticale cultivar BRS Harmonia has moderate resistance to leaf rust, is outstanding in grain yield and quality, and its flour is suitable for cookie baking. It is an excellent alternative as winter crop for the different grain production systems in southcentral Brazil

Automated information extraction from free-text EEG reports

In this study we have developed a supervised learning to automatically detect with high accuracy EEG reports that describe seizures and epileptiform discharges. We manually labeled 3,277 documents as describing one or more seizures vs no seizures, and as describing epileptiform discharges vs no epileptiform discharges. We then used Naïve Bayes to develop a system able to automatically classify EEG reports into these categories. Our system consisted of normalization techniques, extraction of key sentences, and automated feature selection using cross validation. As candidate features we used key words and special word patterns called elastic word sequences (EWS). Final feature selection was accomplished via sequential backward selection. We used cross validation to predict out of sample performance. Our automated feature selection procedure resulted in a classifier with 38 features for seizure detection, and 23 features for epileptiform discharge detection. The average [95% CI] area under the receiver operating curve was 99.05 [98.79, 99.32]% for detecting reports with seizures, and 96.15 [92.31, 100.00]% for detecting reports with epileptiform discharges. The methodology described herein greatly reduces the manual labor involved in identifying large cohorts of patients for retrospective neurophysiological studies of patients with epilepsy