Facile Synthesis of Highly Uniform Fe-MIL-88B Particles

Highly uniform Fe-MIL-88B micron particles with shape evolution from hexagonal bipyramids to bipyramidal hexagonal prism were obtained by a surfactant (polyvinylpyrrolidone, PVP) assisted modified solvothermal method. The modified solvothermal method further demonstrated its feasibility to produce highly uniform micron or nanosized MOF, which provides great opportunities for fabrication of new MOFs and investigation their potential applications in versatile research fields

Impact of interaction style and degree on the evolution of cooperation on Barabási–Albert scale-free network - Fig 8

<p><b>Average payoff</b><i>U</i>(<i>k</i>) <b>of a player of degree</b><i>k</i><b>in the stationary regime of evolutionary dynamics under (a)</b><i>W</i> = 4, <i>b</i> = 1.01, <b>(b)</b> <i>W</i> = 6, <i>b</i> = 1.2 <b>and (c)</b> <i>W</i> = 8, <i>b</i> = 1.3. Cooperator is denoted by C with black and defector by D with red.</p

Probability <i>P</i>_<i>C</i>(<i>k</i>) of a player of degree <i>k</i> playing as a cooperator in the stationary regime of evolutionary dynamics.

<p>The random selection rule is applied and <i>l</i> = 20.</p

Representative result of high-throughput screening of BSH inhibitors.

<p>(A) Plate layout for screening BSH inhibitors. Pink boxes (columns 3–22) indicate test wells that contain library compounds of interest, BSH, and reaction mix containing substrate. Library compounds were shot into the well bottom using Echo 550/555 and enzyme and reaction mix were added using Multidrop Combi. Controls were added manually to the side wells (columns 1–2 and 23–24): blue boxes indicate activity controls (BSH, reaction mix containing substrate, and solvent DMSO), yellow boxes correspond to inhibition controls (BSH, reaction mix containing substrate, and NaIO₃), and white boxes are negative controls with no BSH added but include reaction mix as well as substrate. (B) The HTS results represented by one 384-well plate. Control wells indicate the assay proceeded normally. The wells in columns 3–22 that appeared clear, regardless of alternative color due to compound, and had low absorbance readings were considered hits (putative BSH inhibitors).</p

Interaction relations among all cooperators in the stationary regime of evolutionary dynamics under upper-bound <i>W</i> = 4.

<p>Defect temptation is set to <i>b</i> = 1.01.</p

Effect of selected HTS hits on BSH activity^a.

<p>^a Unless specified, the final concentration of compound in the reaction mix was 5 mM to achieve optimal resolution with the quantitative BSH activity assay.</p><p>^b The final concentration of riboflavin in reaction mix was 1 mM.</p><p>^c The final concentration of chrysophanol in reaction mix was 1.25 mM.</p><p>^d The final concentration of folic acid in reaction mix was 1.5 mM.</p

Density of cooperators <i>P</i>_<i>C</i> on scale-free networks as a function of defection temptation <i>b</i> for different upper-bounds <i>W</i> under a standard PDG parameter combination <i>R</i> = 1, <i>P</i> = 0, <i>S</i> = −0.1,2.1 > <i>b</i> > 1.

<p>Population size is set as <i>N</i> = 1000. <i>R</i> and <i>H</i> correspond to random interaction and high-degree interaction, respectively. The result on corresponding regular ring-<i>W</i> networks is denoted by the dotted line with the same color.</p

The interacting process of players.

<p>The interacting process of players.</p

Effect of different classes of antibiotics on BSH activity.

<p>The antibiotic hits by HTS.</p

Dosing effects of selected BSH inhibitors on BSH activity.

<p>(A) Inhibition of BSH activity by caffeic acid phenethyl ester (CAPE). (B) Inhibition of BSH activity by riboflavin. All assays were performed in triplicate. The procedure is detailed in Materials and Methods.</p