3 research outputs found
Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin
Elucidating
how molecules bind to HSA is fundamental for predicting
drug incompatibilities. Through combinatorial screening, we identified
a novel fluorescent dye (<b>BD140</b>) with turn-on fluorescence
emission and specific binding at HSA drug site 2. We further combined
it with dansylamide to develop a fluorescent dye cocktail for high-throughput
mapping of the interaction between therapeutics at HSA drug-binding
sites
High-Efficiency in Vitro and in Vivo Detection of Zn<sup>2+</sup> by Dye-Assembled Upconversion Nanoparticles
Development
of highly sensitive and selective sensing systems of
divalent zinc ion (Zn<sup>2+</sup>) in organisms has been a growing
interest in the past decades owing to its pivotal role in cellular
metabolism, apoptosis, and neurotransmission. Herein, we report the
rational design and synthesis of a Zn<sup>2+</sup> fluorescent-based
probe by assembling lanthanide-doped upconversion nanoparticles (UCNPs)
with chromophores. Specifically, upconversion luminescence (UCL) can
be effectively quenched by the chromophores on the surface of nanoparticles
via a fluorescence resonant energy transfer (FRET) process and subsequently
recovered upon the addition of Zn<sup>2+</sup>, thus allowing for
quantitative monitoring of Zn<sup>2+</sup>. Importantly, the sensing
system enables detection of Zn<sup>2+</sup> in real biological samples.
We demonstrate that this chromophore–UCNP nanosystem is capable
of implementing an efficient in vitro and in vivo detection of Zn<sup>2+</sup> in mouse brain slice with Alzheimer’s disease and
zebrafish, respectively
High-Efficiency in Vitro and in Vivo Detection of Zn<sup>2+</sup> by Dye-Assembled Upconversion Nanoparticles
Development
of highly sensitive and selective sensing systems of
divalent zinc ion (Zn<sup>2+</sup>) in organisms has been a growing
interest in the past decades owing to its pivotal role in cellular
metabolism, apoptosis, and neurotransmission. Herein, we report the
rational design and synthesis of a Zn<sup>2+</sup> fluorescent-based
probe by assembling lanthanide-doped upconversion nanoparticles (UCNPs)
with chromophores. Specifically, upconversion luminescence (UCL) can
be effectively quenched by the chromophores on the surface of nanoparticles
via a fluorescence resonant energy transfer (FRET) process and subsequently
recovered upon the addition of Zn<sup>2+</sup>, thus allowing for
quantitative monitoring of Zn<sup>2+</sup>. Importantly, the sensing
system enables detection of Zn<sup>2+</sup> in real biological samples.
We demonstrate that this chromophore–UCNP nanosystem is capable
of implementing an efficient in vitro and in vivo detection of Zn<sup>2+</sup> in mouse brain slice with Alzheimer’s disease and
zebrafish, respectively