3 research outputs found

    Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin

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    Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (<b>BD140</b>) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites

    High-Efficiency in Vitro and in Vivo Detection of Zn<sup>2+</sup> by Dye-Assembled Upconversion Nanoparticles

    No full text
    Development of highly sensitive and selective sensing systems of divalent zinc ion (Zn<sup>2+</sup>) in organisms has been a growing interest in the past decades owing to its pivotal role in cellular metabolism, apoptosis, and neurotransmission. Herein, we report the rational design and synthesis of a Zn<sup>2+</sup> fluorescent-based probe by assembling lanthanide-doped upconversion nanoparticles (UCNPs) with chromophores. Specifically, upconversion luminescence (UCL) can be effectively quenched by the chromophores on the surface of nanoparticles via a fluorescence resonant energy transfer (FRET) process and subsequently recovered upon the addition of Zn<sup>2+</sup>, thus allowing for quantitative monitoring of Zn<sup>2+</sup>. Importantly, the sensing system enables detection of Zn<sup>2+</sup> in real biological samples. We demonstrate that this chromophore–UCNP nanosystem is capable of implementing an efficient in vitro and in vivo detection of Zn<sup>2+</sup> in mouse brain slice with Alzheimer’s disease and zebrafish, respectively

    High-Efficiency in Vitro and in Vivo Detection of Zn<sup>2+</sup> by Dye-Assembled Upconversion Nanoparticles

    No full text
    Development of highly sensitive and selective sensing systems of divalent zinc ion (Zn<sup>2+</sup>) in organisms has been a growing interest in the past decades owing to its pivotal role in cellular metabolism, apoptosis, and neurotransmission. Herein, we report the rational design and synthesis of a Zn<sup>2+</sup> fluorescent-based probe by assembling lanthanide-doped upconversion nanoparticles (UCNPs) with chromophores. Specifically, upconversion luminescence (UCL) can be effectively quenched by the chromophores on the surface of nanoparticles via a fluorescence resonant energy transfer (FRET) process and subsequently recovered upon the addition of Zn<sup>2+</sup>, thus allowing for quantitative monitoring of Zn<sup>2+</sup>. Importantly, the sensing system enables detection of Zn<sup>2+</sup> in real biological samples. We demonstrate that this chromophore–UCNP nanosystem is capable of implementing an efficient in vitro and in vivo detection of Zn<sup>2+</sup> in mouse brain slice with Alzheimer’s disease and zebrafish, respectively
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