TRANSFORMASI LEARNING CENTER MANJADI CORPORATE UNIVERSITY: Studi Kasus : Strategi Pengelolaan dan Pengembangan Organisasi Pembelajar Pada Telkom Corporate University

Transformasi Learning Center menjadi Telkom Corporate University (TCU) merupakan tonggak dan fokus Telkom dalam invest in people untuk Pengembangan Sumber Daya Manusia sebagai penentu hidup korporat dari tajamnya persaingan bisnis Telekomunikasi, informasi, media, edutainment, service solution (TIMES), dengan membangun center of excellence. sebagai organisasi pembelajar, yang merupakan strategi human capital dalam meningkatkan daya saing korporat. Tujuan Penelitian ini untuk mengetahui: peran penting TCU-Center of Excellence, Pengelolaan proses dengan pendekatan manajemen strategic Balance Scorecard TCU, strategi budaya Korporat dalam pengembangan TCU yang bakal mencetak para eksekutif muda berbakat dengan kelembutan spiritual yang siap go global. Penelitian ini merekomendasikan TCU, untuk lebih memanfaatkan pengelolaan pengetahuan Learning Organization menuju Teaching Organization sebagai Leader-Driven Organization (organisasi pencetak para pemimpin berbakat), tentu dengan memanfaatkan strategi budaya korporat dan manajemen kinerja ekselen agar gugus knowledge Management menjadi “Brain Center”, sebagai dapur percepatan pembelajaran & pengembangan SDM, baik sebagai human capital dan human energy maupun manfaat bagi Succession Planning. Transformasi sekarang adalah sasaran antara untuk menyiasati layanan bisnis era customer centric ini guna memenangkan lomba-lomba kebaikan layanan TIMES di era customer intention centric dengan lingkungan yang berubah lebih cepat, tiada terprediksi dan turbolensi ditengah kemanjaan ragam customer need & want. Transforming the Learning Center to Telkom Corporate University (TCU) represents a Telkom’s milestone and focus in people investment (invest in people) for the development of human resources which is the determinant of corporate survival due to strict competition in telecommunication, information, media, edutainment, and service solution (TIMES) business. As a learning organization, this is faced by Telkom by building a center of excellence which represents a human capital strategy for improving its corporate competitiveness. The objective of this research is to find out the pivotal role played by TCU center of excellence, process management by TCU Balanced Scorecard strategic management approach, corporate culture strategy for developing TCU to produce talented young executives with spiritual excellence ready to go global. This research recommends TCU as integral part of Telkom to make more use of the Learning Center’s knowledge management aiming at Teaching Orgainization namely as Leader Driven Orgainization producing talented leaders, by making use of corporate culture strategy and excellent performance management in order to transform the knowledge management unit to become a “Brain Center” functioning as a human resources learning and developing accelerator both as human capital and human energy as well as benefit for successsion planning. The current transformation is an intermediate objective to reengineer business service in the customer centric era and to win the competition in TIMES services in the customer intention centric era with unpredictable, turbulent, rapid changing environment amid the customer’s numerous ever developing need and want

Treatment of adult female <i>B. malayi</i> with <i>Bm-cpl-1</i>, <i>Bm-cpl-5</i>, and <i>Bm-cpz</i> dsRNAs leads to phenotypic changes in developing embryos.

<p>Intrauterine progeny from individual female worms were examined 2 d after treatment with medium control (A), <i>Ov-cpz-Int2</i> control dsRNA (B), <i>Bm-cpl-5</i> dsRNA (C) and <i>Bm-cpl Pro</i> dsRNA (D).</p

Demonstration of uptake of Cy3-labeled dsRNAs or siRNA by <i>B. malayi</i>.

<p>Adult female <i>B. malayi</i> (2 groups of 4 worms) were soaked in normal culture medium containing Cy3-dsRNA (0.01 mg/ml) for 24–72 h. Uptake was examined for <i>Bm-cpl-5</i> dsRNA (∼800 bp) (A), <i>Bm-cpl-5</i> siRNA (B), <i>Bm-cpl Pro</i> dsRNA (∼400 bp) (C) and <i>Bm-cpl Pro</i> siRNA (D). Fluorescence was visualized using a Zeiss Axiovert fluorescence microscope using the rhodamine filter set, using emission 590 nm.</p

dsRNA-mediated silencing of the <i>B. malayi</i> cathepsin-like genes, <i>Bm-cpl-1</i>, <i>Bm-cpl-5</i>, and <i>Bm-cpz</i> leads to a reduction in microfilaria release from <i>B. malayi in vitro</i>.

<p>Following 24 h culture in normal culture medium, two groups of 3 female <i>B. malayi</i> worms were soaked for 18 h in 2 mg/ml gene-specific dsRNA (<i>Bm-cpl-1</i>, <i>Bm-cpl-5</i>, <i>Bm-cpz</i>, <i>Ov-cpz-Int2</i>) or medium alone (control). Following dsRNA treatment, individual worms were transferred to dsRNA-free medium and cultured for an additional 48 h. Released microfilariae were collected and counted daily. Results are expressed as Mf release before RNAi treatment (A), 24 h (B) and 48 h (C) after treatment. Each graph represents one experiment which is representative of at least 3 separate experiments. <i>P</i> values denote a significant difference between dsRNA-treated worms and either untreated medium controls or negative control (<i>Ov-cpz-Int2</i>) (Mann-Whitney <i>U</i>-test).</p

GenBank accession numbers, and primer sequences and positions used for gene specific dsRNA production and real-time RT-PCR (qRT-PCR) with the resulting fragment size.

<p>GenBank accession numbers, and primer sequences and positions used for gene specific dsRNA production and real-time RT-PCR (qRT-PCR) with the resulting fragment size.</p

Embryogenesis effects following treatment of adult female <i>B. malayi</i> with dsRNA or siRNA.

<p>Intrauterine progeny from individual female worms (groups of four adult worms) were examined 2 d after dsRNA treatment and expressed as the relative proportions of progeny at different stages of development. Data represents one representative experiment from at least 3 separate experiments.</p

Chitin and chitosan measured in <i>C. elegans</i> tissues.

<p>Chitin and chitosan measured in <i>C. elegans</i> tissues.</p

Nematode PDAs Identified Through Bioinformatics using resources from NCBI, Sanger, and www.nematode.net.

a,b<p>The sequences ADY42356 and ADY43217 share 99% sequence identity within the predicted NodB homology domain, but only 32% sequence identity outside this region. ADY42356 has a truncated portion of the catalytic domain. We are unable to discern these as 1 or 2 homologs, but given the catalytic domain identity we use only ADY43217 for alignments.</p>c<p>Based on our sequencing of this region of the gene we have shown that predicted residues 1534–1556 (relative to predicted sequence associated with Accession No. CCD67046) would not be present in the protein sequence.</p>d<p><i>M. incognita</i> Msp9 is a potential homolog which has sequence verification from two clones available from <a href="http://www.nematode.net" target="_blank">www.nematode.net</a>. Msp30 (AY142120) shows some homology as well, but there is less supporting data.</p

Developmental time course of F48E3.8 and C54G7.3 gene expression in germline-ablated <i>C. elegans</i> using RT-PCR.

<p>Synchronous non-permissive (germline-ablated) worms of this strain were grown on solid media and used to collect RNA from various time-points following L1 arrest at hatching and continuing into adulthood. First strand cDNA (+) was generated from each RNA extract (including No RT controls, –) and used as a template for PCR to analyze expression of the genes F48E3.8, C54G7.3, the eggshell chitin synthase <i>chs-1</i> and the pharyngeal chitin synthase <i>chs-2</i>. The gene <i>ama-1</i>, encoding the large subunit of RNA polII, was used as a positive control (as done by Johnstone and Barry 1996 and Veronico <i>et. al.</i> 2001) although we note that expression of the gene is not consistent as previously described. Ablation of the germline was confirmed by the attenuation in expression of <i>chs-1</i>. The transition to adult stages was confirmed by the expression of <i>col-19</i>, an adult specific collagen gene. All primers and PCR conditions are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040426#pone.0040426.s002" target="_blank">Table S2</a>.</p

Potential roles for nematode PDAs.

<p>Potential roles for nematode PDAs.</p