26 research outputs found

    Expression of miRNA precursor and its major mature form in 15 mouse tissues.

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    <p>A heat map was constructed using GenePattern software based on the normalized miRNA reads. Heat maps showed precursors and major forms to have similar patterns of expression.</p

    Expression of mature miRNAs in 16 mouse tissues.

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    <p>Expression analysis was performed using real-time RT-PCR. The expression levels were normalized to U6 snRNA. (a) miR-194-5p expression, (b) miR-192-5p expression, (c) miR-215-5p expression. * Preferential expressed tissues identified by ANOVA (<i>P</i><0.05) and Turkey's HSD test</p

    Expression of the miRNA precursor and its major and minor mature forms in 15 mouse tissues.

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    <p>Heat maps were constructed using GenePattern software based on the normalized miRNA reads. This heat map shows precursors and minor forms to have different patterns of expression in many paralogous families.</p

    Tissue coexpression scores of major/minor pairs.

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    <p>Selection was based on the score ranked top 10% and Fisher exact test between minor forms with <i>P</i> values below 0.05 in particular tissues. If the minor form did not have any reads, then the coexpression score of its major/minor pair was considered to be zero.</p><p>BM: bone marrow, Thy: thymus, Spl: Spleen, Lym: lymph, Bra: brain, Lung: lung, Liv: Liver, Kid: kidneys, Pan: pancreas, Ski: skin, Sal: salivary glands, Tes: testes, Ova: ovary.</p

    Locations and expressions of the host genes and minor forms of mouse miR-128-1, 2 in 16 mouse tissues.

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    <p>(a) Structure of R3hdm1 as miR-128-1 host gene. (b) Structure of Arpp21 as miR-128-2 host gene. (c) qRT-PCR analysis of R3hdm1 expression, the levels of expression were normalized to 18S rRNA. (d) qRT-PCR analysis of Arpp21 expression, the levels of expression were normalized to 18S rRNA. (e) qRT-PCR analysis of the expression of miR-128-1-5p and miR-128-2-5p, the levels of expression were normalized to U6 snRNA. * Preferential expressed tissues identified by ANOVA (<i>P</i><0.05) and Turkey's HSD test.</p
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