Additional file 1 of First case of endometrial cancer after yolk sac tumor in a patient with Li-Fraumeni syndrome

Additional file 1: Supplementary Table 1. The genes list of the 688 genes detected in this case

Additional file 1 of Systematic profiling of alternative splicing signature reveals prognostic predictor for cervical cancer

Additional file 1: Table S1. Alternative splicing events in 253 cancer samples

The antibody response induced by rTgPDI vaccine immunisation.

<p>Sera were collected from the BALB/c mice immunised with different doses of rTgPDI or PBS two weeks after the final inoculation. (A) Determination of the levels of specific anti-rTgPDI IgG in the sera of BALB/c mice immunised with different doses of rTgPDI or PBS under the same conditions. Results are expressed as the mean OD₄₅₀± SD (n = 10) and are representative of three individual experiments. (B) The levels of both IgG1- and IgG2a-specific anti-rTgPDI isotype antibodies in the sera of mice immunised with different doses of rTgPDI or PBS. Results are expressed as the mean OD₄₅₀± SD (n = 10) and are representative of three individual experiments. The results indicated that rTgPDI could induce IgG production and a Th1-type response. **<i>P</i><0.01 compared with the control group.</p

Additional file 2 of Systematic profiling of alternative splicing signature reveals prognostic predictor for cervical cancer

Additional file 2: Table S2. Integrated clinical follow-up data of cervical cancer patients

Alignment of the amino acid sequence of TgPDI.

<p>The alignment of the amino acid sequence of PDI from three strains(RH, ME49 and VEG strain)of <i>T. gondii</i> was performed by DNAMAN (Lynnon, Quebec, Canada). Homology levels are highlighted in colors. Black: 100%.</p

Survival of the immunised mice after <i>T. gondii</i> challenge.

<p>Two groups of mice (16 mice per group) were immunised with 30 μg of rTgPDI or PBS through intranasal administration at 0, 14 and 21 days, and on the 15th day after the last immunisation, the mice were orally challenged with 4×10⁴<i>T. gondii</i> tachyzoites from the RH strain. The survival of the mice was monitored daily for 30 days after challenge. The results showed that treatment with 30 μg of rTgPDI prolonged the survival of immunised BALB/c mice compared to the controls.</p

Additional file 3 of Systematic profiling of alternative splicing signature reveals prognostic predictor for cervical cancer

Additional file 3: Table S3. Prognosis-related alternative splicing events of cervical cancer

SDS-PAGE and Western blotting analyses of the rTgPDI protein.

<p>(A) The full-length ORF of TgPDI was expressed in <i>E. coli</i>, then separated on 12% SDS-PAGE gels and stained with Coomassie blue. The molecular weight of rTgPDI was approximately 57 KDa. (B) Purified rTgPDI protein was detected via 12% SDS-PAGE and staining with Coomassie blue; the purity of rTgPDI was greater than 95%. (C) Western blotting analysis of rTgPDI using a rabbit anti-<i>T. gondii</i> serum polyclonal antibody (upper) and anti-His antibody (lower); rTgPDI could be recognised by both the His antibody and anti-<i>T. gondii</i> serum. (D) Western blotting analysis of purified rTgPDI using an anti-<i>Toxoplasma</i> human serum. Lane 1 and 2 represented 2 μg and 5 μg purified rTgPDI respectively. (E) Western blotting analysis of native TgPDI using the antibodies elicited from mouse immunised with rTgPDI. Lane 1 and 2 represented 20 μg and 50 μg STAg respectively.</p

The burden of tachyzoites in immunised mice.

<p>The brains and livers of immunised mice and control mice were collected, and the tachyzoite burdens were quantified via microscopy. Results are expressed as the mean ± SD (n = 10) and represent numbers of tachyzoites per gram of tissue. The data showed that rTgPDI could reduce the tachyzoite burdens in the brain and liver compared with the control group. *<i>P</i><0.05 compared with the control group.</p

Cytokine production in splenocytes from immunised mice.

<p>The culture supernatants were examined by ELISA for cytokine production. The IL-2 and IL-4 levels were determined after 24 h of culturing; IL-10 activity after 72 h; and IFN-γ levels after 96 h. The results showed that rTgPDI (especially the 30 μg dosage) increased the levels of IFN-γ, IL-2 and IL-4, but not IL-10 compared with the PBS control. *<i>P</i><0.05 and **<i>P</i><0.01 compared with the control group.</p