Vibration interference in SIM imaging of LIPSS in experiments without antivibration

Vibration interference in SIM imaging of LIPSS in experiments without antivibratio

Supplementary document for Temporal modulation of nonlinear ionization process in femtosecond laser pulse induced periodic surface structures - 6117294.pdf

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Bridging Ionic Current Rectification and Resistive-Pulse Sensing for Reliable Wide-Linearity Detection

As two mainstream ionic detection techniques, ionic current rectification (ICR) suffers from large fluctuations in trace level detection, while resistive-pulse sensing (RPS) encounters easy clogs in high-concentration detection. By rationally matching the nanopore size with the DNA tetrahedron (TDN), this work bridges the two techniques to achieve reliable detection with wide linearity. As a representative analyte, miRNA-10b could specifically combine with and release TDN from the interior wall, which thus induced the simultaneous generation of distinct ICR and RPS signals. The ICR signals could be attributed to the balance between the effective orifice and surface charge density of the inner wall, while the RPS signals were induced by the complex of miRNA-10b and TDN passing through the nanopore. Such an operation contributed to a wide detection range of 1 fM–1 nM with a good linearity. The feasibility of this method is also validated in single-cell and real plasma detection

Measurement and Correlation of Isobaric Vapor–Liquid Equilibrium for Binary Systems of 1‑(Ethoxymethoxy)-2-methyl-propane with Isobutyl Alcohol or 1‑Butanol at 101.33 kPa

Experimental vapor–liquid equilibrium (VLE) data for binary systems of 1-(ethoxymethoxy)-2-methyl-propane (EMMP) with isobutyl alcohol and 1-butanol at 101.33 kPa was measured. The measurements of saturated vapor pressure for EMMP were also measured. The experiment was performed by an improved Rose still. A minimum boiling azeotrope was found about the binary system containing 1-butanol for which the azeotropic temperature and composition are 389.64 K and 75.92 mol% (1-butanol) at 101.33 kPa. The VLE measurements were correlated by the Wilson, nonrandom two-liquid, and universal quasichemical models for which the results showed that the measurements had a good correlation by using three models about two binary systems, respectively. The thermodynamic consistency of the VLE measurements was checked by the traditional area test and the direct test methods

Genome-wide identification and classification of <i>MYB</i> superfamily genes in peach

<div><p>The MYB transcription factor superfamily is one of the largest superfamilies modulating various biological processes in plants. Over the past few decades, many <i>MYB</i> superfamily genes have been identified and characterized in some plant species. However, genes belonging to the MYB superfamily in peach (<i>Prunus persica</i>) have not been comprehensively identified and characterized although the genome sequences of peach were released several years ago. In total, this study yielded a set of 256 <i>MYB</i> superfamily genes that was divided into five subfamilies: the R2R3-MYB (2R-MYB), R1R2R3-MYB (3R-MYB), MYB-related (1R-MYB), 4R-MYB, and Atypical-MYB subfamilies. These subfamilies contained 128, 4, 109, 1, and 14 members, respectively. The 128 R2R3-MYB subfamily genes in peach were further clustered into 35 groups, and the 109 MYB-related subfamily genes were further clustered into 6 groups: the CCA1-like, CPC-like, TBP-like, I-box-binding-like, R-R-type, and Peach-specific groups. The motif compositions and exon/intron structures within each group within the R2R3-MYB or MYB-related subfamily in peach were highly conserved. The logo sequences of the R2 and R3 repeats of R2R3-MYB subfamily members were highly conserved with those in these repeats of several other plant species. Except for 48 novel peach-specific <i>MYB</i> genes, the remaining 208 out of 256 <i>MYB</i> genes in peach were conserved with the corresponding 198 <i>MYB</i> genes in <i>A</i>. <i>thaliana</i>. Additionally, the 256 <i>MYB</i> genes unevenly distributed on chromosomes 1 to 8 of the peach genome. Eighty-one orthologous pairs of peach/<i>A</i>. <i>thaliana MYB</i> genes were identified among 256 <i>MYB</i> genes in peach and 198 <i>MYB</i> genes in <i>A</i>. <i>thaliana</i> in this study. In addition, 146 pairs of paralogous <i>MYB</i> genes were identified on the eight chromosomes of peach. The expression levels of some of the 51 <i>MYB</i> genes selected for qRT-PCR analysis decreased or increased with red-fleshed fruit development, while the expression patterns of some genes followed no clear rules over the five developmental stages of fruits. This study laid the foundation for further functional analysis of <i>MYB</i> superfamily genes in peach and enriched the knowledge of <i>MYB</i> superfamily genes in plant species.</p></div

Logo sequences of R2 and R3 repeats of R2R3-MYB subfamily members in peach.

<p>The logo sequences of motifs 3, 4, 1, and 2, which together constitute the R2 and R3 repeats in peach. The overall height of each stack represents the conservation of the sequence at that position. The capital letters indicate greater than 50% conservation of amino acids among the MYB domains. The Arabic numerals under the colored capital letters indicate the position of each residue and the width of the motif. Each color of the English letters represents a different type of amino acid residue.</p

Motif distribution of peach MYB-related (1R-MYB), 4R-MYB, and Atypical-MYB subfamily proteins.

<p>Motifs of the 1R-MYB, 4R-MYB, and Atypical-MYB subfamily proteins were analyzed using the MEME web server. Fifteen colors of blocks represent the 15 types of motifs. The length of the gray line indicates the length of a sequence relative to those of all the other sequences. The position of each block indicates the location of a motif with a matching sequence. The red brace on the right marks the members of each group of the 1R-MYB, 4R-MYB, and Atypical-MYB subfamilies in peach.</p

Table_1_Identification of Odorant-Binding Proteins (OBPs) and Functional Analysis of Phase-Related OBPs in the Migratory Locust.PDF

<p>Olfactory plasticity, which is one of the major characteristics of density-dependent phase polyphenism, plays critical roles in the large-scale aggregation formation of Locusta migratoria. It is still unknown whether odorant-binding proteins (OBPs) are involved in phase-related olfactory plasticity of locusts, despite the confirmed involvement of several types of olfactory perception genes. In this study, we performed a large-scale search for OBPs and verified their expression patterns in the migratory locust. We identified 17 OBPs in the L. migratoria genome, of which 10 were novel, and we found their scattering distribution characteristics by mapping the genomic loci. Next, we revealed that these OBPs with close phylogenic relationships displayed similar tissue-specific expression profiles by a combined analysis of qRT-PCR and phylogenetic tree reconstruction. In all identified locust OBPs, seven OBPs showed differential mRNA expression levels in antenna tissue between gregarious and solitarious nymphs. Six of these seven OBPs displayed higher mRNA expression in the antennae of gregarious nymphs. The mRNA expression of LmigOBP2 and LmigOBP4 increased during gregarization and decreased during solitarization. RNAi experiments confirmed that only LmigOBP4 regulates the behavioral traits to affect gregarious behavior. These results demonstrated that OBPs also play important roles in the regulation of phase-related behavior of the locusts.</p

Phylogenetic tree of R2R3-MYB and R1R2R3-MYB (3R-MYB) subfamilies of <i>A</i>. <i>thaliana</i> and peach MYB proteins.

<p>The phylogenetic tree was constructed for R2R3-MYB and R1R2R3-MYB subfamilies using the sequences of 131 R2R3-MYB and R1R2R3-MYB proteins in <i>A</i>. <i>thaliana</i> and 256 MYB proteins of peach. The small red circles represent the 256 peach MYB proteins; the small green triangles represent the 126 R2R3-MYB subfamily and 5 R1R2R3-MYB subfamily proteins from <i>A</i>. <i>thaliana</i>. The English letters with Arabic numbers outside the large red circle indicate the names of each group of the peach R2R3-MYB and R1R2R3-MYB subfamilies. The red dotted line represents the initial or final boundary of each group of the peach R2R3-MYB and R1R2R3-MYB subfamilies.</p

Motif distribution of peach R2R3-MYB subfamily and R1R2R3-MYB (3R-MYB) subfamily proteins.

<p>Motifs of the R2R3-MYB and R1R2R3-MYB subfamily proteins were analyzed using the MEME web server. Fifteen kinds of colored blocks represent 15 kinds of motifs. The length of the gray line indicates the length of a sequence relative to that of all the other sequences. The position of each block indicates the location of a motif with a matching sequence. The red brace on the right marks the members of each group of the R2R3-MYB and R1R2R3-MYB subfamilies in peach.</p