Table_2_The cross-sectional relationship between vitamin C and high-sensitivity C-reactive protein levels: insights from NHANES database.DOCX

BackgroundAscorbic acid or vitamin C has antioxidant and anti-inflammatory properties that may impact markers of inflammation like C-reactive protein (CRP). However, studies specifically on vitamin C and high-sensitivity CRP (hs-CRP) have been scarce.MethodsWe conducted a cross-sectional analysis of the National Health and Nutrition Examination Survey 2017–2018 dataset including 5,380 U.S. adults aged ≥20 years. Multiple regression models examined the relationship between plasma vitamin C and serum hs-CRP while adjusting for potential confounders. Stratified analyses and curve fitting assessed effect modification and nonlinearity.ResultsAn inverse association was found between plasma vitamin C and serum hs-CRP overall (β = −0.025, 95% CI: −0.033 to −0.017, p ConclusionThe results showed a non-linear negative correlation between vitamin C levels and hs-CRP in adults. These results suggest vitamin C intake may reduce inflammation and cardiovascular risk, but only up to 53.1 μmol/L plasma vitamin C.</p

Table_1_The cross-sectional relationship between vitamin C and high-sensitivity C-reactive protein levels: insights from NHANES database.docx

BackgroundAscorbic acid or vitamin C has antioxidant and anti-inflammatory properties that may impact markers of inflammation like C-reactive protein (CRP). However, studies specifically on vitamin C and high-sensitivity CRP (hs-CRP) have been scarce.MethodsWe conducted a cross-sectional analysis of the National Health and Nutrition Examination Survey 2017–2018 dataset including 5,380 U.S. adults aged ≥20 years. Multiple regression models examined the relationship between plasma vitamin C and serum hs-CRP while adjusting for potential confounders. Stratified analyses and curve fitting assessed effect modification and nonlinearity.ResultsAn inverse association was found between plasma vitamin C and serum hs-CRP overall (β = −0.025, 95% CI: −0.033 to −0.017, p ConclusionThe results showed a non-linear negative correlation between vitamin C levels and hs-CRP in adults. These results suggest vitamin C intake may reduce inflammation and cardiovascular risk, but only up to 53.1 μmol/L plasma vitamin C.</p

Table_1_Egg yolk antibody combined with bismuth-based quadruple therapy in Helicobacter pylori infection rescue treatment: a single-center, randomized, controlled study.pdf

BackgroundThe increasing antibiotic resistance is the main issue causing Helicobacter pylori (H. pylori) eradication failure. As a nutritional supplement, Egg Yolk Antibody (Ig Y) provides a new approach for H. pylori infection rescue therapy.MethodsIn this randomized, controlled study, 100 H. pylori-positive patients with previous H. pylori eradication treatment were included. All individuals received standard bismuth-containing quadruple therapy twice daily (5 mg ilaprazole, 100 mg doxycycline, 500 mg clarithromycin or 1 g amoxicillin or 100 mg furazolidone, and 220 mg colloidal bismuth tartrate) for 14 days and were randomized to receive either twice daily 7 g Ig Y-H. pylori treatment (study group) or not (control group). 4 weeks after the end of treatment, urea breath tests were used to assess the H. pylori eradication rate. All participants scored by the Global Overall Symptom scale (GOS) and recorded adverse events during the trial.ResultsThe H. pylori eradication rates were 84.0% (95% CI 73.5–94.5%) vs. 80.0% (95% CI 68.5–91.5%) in the study and control groups at intention-to-treat (ITT) analysis and 85.7% (95% CI 75.6–95.9%) vs. 80.0% (95% CI 68.5–91.5%) at per-protocol (PP) analysis, respectively. The number of over 80% symptom relief after treatment in the two groups was 27 (60%) and 12 (29.2%) (p ConclusionBoth groups achieved satisfactory eradication efficiency in H. pylori rescue therapy and Ig Y-H. pylori effectively alleviates the symptoms with good compliance and fewer adverse effects.</p

Characterization of the <i>osfie2-1</i> mutant.

<p>A, The phenotype of the wild-type (<i>left</i>) and <i>osfie2-1</i> (<i>right</i>) during the seedling stage. B, Phenotype of wild-type and <i>osfie2-1</i> during the tillering stage. C, Phenotype during the reproductive stage. D, Panicles of wild-type (left) and <i>osfie2-1</i> (right). E, Spikelet of wild-type. F-I, Aberrant spikelet of <i>osfie2-1</i>. J, The grain phenotype of the wild-type (up) and <i>osfie2-1</i> (down). K-N, semi-thin section of <i>osfie2-1</i> and wild-type hulls. O-V, Quantification of the phenotypic analysis of wild-type and <i>osfie2-1</i>. Data are given as mean ± SD. Student’s t-test was used to generate the P values; * and ** indicate P<0.05 and P<0.01, respectively. Scale bars: 5 cm (A, B); 10 cm (C, D); 0.25 cm (E-I); 0.5 cm (J); 0.05 cm (K-N).</p

Interaction of OsFIE2 and OsiEZ1.

<p>A, Yeast two-hybrid assay showing OsFIE2 interacting with OsiEZ1. The co-transformations with prey and bait were examined on the control media–LT (SD-Trp/-Leu) and the interactions between bait and prey were performed on selective media–LTHA (SD-His/-Trp/-Leu/-Ade/-His) plus X-α-gal. AD, activating medium; BD, binding domain; SD, synthetic dropout. B, Bimolecular fluorescence complementation analysis showing OsFIE2 interacting with OsiEZ1. eYFP, enhanced yellow fluorescent protein. BF, bright-filed image. NY and CY indicate the N terminus and C terminus of eYFP, respectively.</p

RNA-seq-based and qPCR analysis of differentially expressed genes.

<p>A, GO enrichment analysis of differentially expressed genes (DEGs). B, Heatmap showing the expression levels of DEGs in <i>osfie2-1</i> according to RNA-seq. C, Expression level of homeobox <i>OsMADS-BOX</i> genes and <i>Oskn3</i> in wild-type and <i>osfie2-1</i>. D, Expression level of phytohormone-related genes. E-I, Phytohormones content in wild-type and <i>osfie2-1</i>. Data are given as mean ± SD of three biological replicates. Student’s t-test was used to generate the P values; * and ** indicate P<0.05 and P<0.01, respectively.</p

Circular RNAs Mediate the Effects of Dietary Tryptophan on the Transformation of Muscle Fiber Types in Weaned Piglets

The nutritional composition of the diet significantly impacts the overall growth and development of weaned piglets. The current study aimed to explore the effects and underlying mechanisms of dietary tryptophan consumption on muscle fiber type transformation during the weaning period. Thirty weaned piglets with an average body weight of 6.12 ± 0.16 kg were randomly divided into control (CON, 0.14% Trp diet) and high Trp (HT, 0.35% Trp) groups and maintained on the respective diet for 28 days. The HT group of weaned piglets exhibited highly significant improvements in growth performance and an increased proportion of fast muscle fibers. Transcriptome sequencing revealed the potential contribution of differentially expressed circular RNAs toward the transformation of myofiber types in piglets and toward the regulation of expression of related genes by targeting the microRNAs, miR-34c and miR-182, to further regulate myofiber transformation. In addition, 145 DE circRNAs were identified as potentially protein-encoding, with the encoded proteins associated with a myofiber type transformation. In conclusion, the current study greatly advances and refines our current understanding of the regulatory networks associated with piglet muscle development and myofiber type transformation and also contributes to the optimization of piglet diet formulation

Subcellular localization and expression pattern of <i>OsFIE2</i>.

<p>A, Subcellular localization of OsFIE2-GFP fusion protein in rice protoplasts. Confocal scanning images show localization in the nucleus and cytoplasm. 35S:GFP was used as a positive control. eGFP, enhanced green fluorescent protein. BF, bright-filed image. B, Expression pattern of <i>OsFIE2</i> at the seedling stage. SL, seedling leaves; LS, leaf sheath; SR, seedling roots. C, Expression pattern of <i>OsFIE2</i> at the heading stage. R, roots; C, culms; L, leaves; S, leaf sheaths; P, Panicles. D, Expression pattern of <i>OsFIE2</i> in the endosperm at different stages (3, 6, 9, 12 days after pollination, DAP). Data are given as mean ± SD of three biological replicates.</p

Histological characterization of the stem and spikelet hulls of the <i>osfie2-1</i> mutant.

<p>A, Comparison of main culms of wild-type and <i>osfie2-1</i>, arrows indicate the positions of nodes. B-E, Transverse sections and longitudinal sections of the second internode of the wild-type and <i>osfie2-1</i>; SVB, small vascular bundle; LVB, large vascular bundle. F, The spikelet hulls phenotypes of wild-type and <i>osfie2-1</i>. G-J, Scanning electron microscope analysis of the outer and inner epidermal cells of spikelet hulls in wild-type and <i>osfie2-1</i>. K and L, Number of SVB and LVB calculated from transverse sections of the second internode, (n = 10). M and N, The parenchyma cell (PC) length and width, (n = 12). O-R, Cell length and cell width in outer and inner spikelet hulls, (n = 12). Data are given as mean ± SD. Student’s t-test was used to generate the P values; * and ** indicate P<0.05 and P<0.01, respectively. Scale bars: 15 cm (A); 0.05 mm (B-E); 2 mm (F); 40 μm (G-J).</p

Map-based cloning of <i>osfie2-1</i>.

<p>A, The <i>OsFIE2</i> locus was roughly mapped on the short arm of chromosome 8 between the makers M1 and M2. Fine mapping the <i>OsFIE2</i> locus was then restricted to a 131 kb interval between makers M7 and M8. Thirty one candidates genes are illustrated in this region. The number of recombinations are shown under the maker position. A single base change from C to T was found inside the first exon between wild-type (ZH11) and the <i>osfie2-1</i> mutant. Eight other varieties all have the wild-type allele. B-D Morphology of wild-type, <i>osfie2-1</i> and <i>OsFIE2</i> overexperssion transgenic lines, their panicles and grains. E, Relative expression level of <i>OsFIE2</i> in wild-type, <i>osfie2-1</i> and <i>OsFIE2</i> overexperssion transgenic lines. F and G, Grain length and 1000-grain weight in wild type, <i>osfie2-1</i> and <i>OsFIE2</i> overexperssion transgenic lines. Data are given as mean ± SD. Scale bars: 25 cm (B); 3.5 cm (C); 2.5 mm (D).</p