Protein concentration of aqueous humor.

<p>Protein concentration of aqueous humor.</p

Wafer implantation into the rat anterior chamber.

<p>A. A full thickness corneal incision was made parallel to the plane of the iris (arrows). Wafers were introduced to the anterior chamber using toothless forceps (dotted circle), and displaced to its temporal position (dotted arrow) in the anterior chamber. B. Side-view schematic showing the approximate location of the implant. C. The final position of all implants was an average of 0.8 mm from the limbus (dotted line). Ch = cholesterol, 7KCh = 7-ketocholesterol, n = number of wafers implants.</p

Quantification of VEGF, IL-1β and GRO/KC in AH 4 and 7 days after implantation.

<p>AH from eyes of un-implanted (control) and implanted with 20% Ch and 7KCh wafers were analyzed for cytokine levels 4 and 7 days post implantation, as described above. A. VEGF. B. IL-1β. C. GRO/KC.</p

Localization of CD68 positive cells and VEGF in Ch and 7KCh implants.

<p>Rat eyes were implanted with 20% Ch and 7KCh wafers and cryosections prepared 14 days post implantation. The sections were labeled with AlexaFluor 488 (green) for anti-CD68 or anti-VEGF and the nuclei stained with DAPI (blue). A. Ch implant, anti-CD68. B. 7KCh implant, anti-CD68. C. Ch implant, anti-VEGF. D. 7KCh implant, anti-VEGF.</p

Histological comparison of Ch and 7KCh implants using isolectin IB₄.

<p>Rat eyes were implanted with 20% Ch and 7KCh wafers and cryosections prepared 14 days post implantation. A. Ch implant labeled with isolectin IB₄. B. Ch implant dual labeled with DAPI. C. 7KCh implant labeled with isolectin IB₄. D. 7KCh implant dual labeled with DAPI.</p

Assignment of experimental groups and treatments.

*<p>Special diet refers to calcium, vitamin D, magnesium and zinc deficient diet.</p

SEM images showing preservation of the morphological and structural integrity of the sample before conversion (a,b and c) and after conversion (d, e and f).

<p>The images also detail the uniform distribution of surface pores.</p

SDS-PAGE and immunoblotting of AH from Ch and 7KCh implants.

<p>AH (5 µl) from control (un-implanted eye), Ch and 7KCh implanted eyes were separated by SDS-PAGE and blotted. A. Ponceau S red stained blot demonstrating the difference in protein content between samples. B. Anti-VEGF labeling of blot.</p

Effects of time and 7KCh concentrations on angiogenesis.

<p>Wafers containing 5, 7, 10 and 15% 7KCh (w/w) were implanted into rats anterior chambers as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056099#pone-0056099-g001" target="_blank">Fig. 1</a> and representative images are shown at 7, 10, 14 and 21 days. The vessel volume for each panel is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056099#pone-0056099-g004" target="_blank">Fig. 4b</a>, in mm².</p

The XRD pattern corresponding to <i>foraminifera,</i> β-TCP and Zn-TCP showing peaks matching calcium carbonate (JCPDS 5-0586) and tricalcium phosphate (JCPDS 9-169).

<p>The XRD pattern corresponding to <i>foraminifera,</i> β-TCP and Zn-TCP showing peaks matching calcium carbonate (JCPDS 5-0586) and tricalcium phosphate (JCPDS 9-169).</p