7 research outputs found

    Catalpa ovata G. Don

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    原著和名: キササゲ科名: ノウゼンカズラ科 = Bignoniaceae採集地: 千葉県 千葉市 千葉大学 (下総 千葉市 千葉大学)採集日: 1958/8/1採集者: 萩庭丈壽整理番号: JH026902国立科学博物館整理番号: TNS-VS-97690

    Additional file 1: Figure S1. of Alpha-1-antitrypsin interacts with gp41 to block HIV-1 entry into CD4+ T lymphocytes

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    Purity and quality of AAT, ΔAAT and C. ΔAAT was prepared following the protocol in Materials and. Next, AAT, purified ΔAAT and C were analyzed on 4–20 % Tris-Glycin SDS-PAGE gel and analyzed by in-gel digestion mass fingerprinting assay (A). Aligned sequences were also shown for AAT, ΔAAT and C (B). (TIF 6959 kb

    Additional file 2: Figure S2. of Alpha-1-antitrypsin interacts with gp41 to block HIV-1 entry into CD4+ T lymphocytes

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    AAT, raltegravir, emtricitabine and enfuvirtide had no obvious effect on the viability of CD4+ T cells. CD4+ T cells were pretreated with or without AAT for 1 h. Next, these cells were incubated with AAT, raltegravir (10−4 g/L), emtricitabine (10−3 g/L), or enfuvirtide (10−3 g/L) for indicated time period. At the end of incubation, cells were collected to detect the viability using trypan blue staining. RAL: raltegravir treatment; FTC: emtricitabine treatment; ENF: enfuvirtide treatment. (TIF 975 kb

    Additional file 5: Figure S5. of Alpha-1-antitrypsin interacts with gp41 to block HIV-1 entry into CD4+ T lymphocytes

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    AAT, C and ΔAAT did not directly affect the activity of HIV-1 reverse transcriptase. CD4+ T cells were pretreated in the presence or absence of AAT/C/ΔAAT and then infected by HIV-191US054 (A) or HIV-192US714 (C) without removing the reagents. Infected CD4+ T cells were then washed three times to remove unbound viruses and incubated in the presence or absence of AAT/C/ΔAAT (same condition as before infection) for 1, 6, 12, 18, 24, or 30 h to isolate whole cell and viral proteins. HIV-1 reverse transcriptase activity was detected. Meanwhile, CD4+ T cells were also infected with primary HIV-191US054 (B) or HIV-192US714 (D) without AAT/C/ΔAAT pretreatment and then incubated in the presence or absence of AAT/C/ΔAAT. After 0, 5, 11, 17, 23, or 29 h incubation, whole cell proteins with viral proteins were extracted from these CD4+ T cells to detect the activity of HIV-1 reverse transcriptase. (TIF 1480 kb

    Stimuli-Responsive Amphiphilic Polyelectrolyte Heptablock Copolymer Physical Hydrogels: An Unusual pH-Response

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    An amphiphilic cationic polyelectrolyte based on poly­[2-(dimethylamino)­ethyl methacrylate] (polyDMA) and poly­(<i>n</i>-butyl methacrylate) (polyBuMA) with a BuMA–DMA–BuMA–DMA–BuMA–DMA–BuMA heptablock copolymer architecture was studied in aqueous media. This copolymer was found to form a physical hydrogel via the intermolecular hydrophobic association (physical cross-linking) of the BuMA blocks. The rheological properties of the heptablock hydrogels were investigated as a function of copolymer concentration, and pH. The results showed a peculiar pH-dependence of the rheological properties, remarkably different from those observed with associative telechelic polyelectrolytes. Aqueous solutions of this copolymer were free-flowing sols at low pH (below 2) and high pH (above 8), whereas they turned into gels at intermediate pH values. The rheological properties studied as a function of pH showed two additional stiff–soft–stiff gel transitions at pH 4.5 and 6.5. Small-angle neutron scattering revealed the formation of a 3D transient network of bridged flower-like micelles whose structural characteristics, i.e., micellar radius, hard-sphere radius and hard-sphere volume fraction, were smoothly evolving with the pD

    Genetic and clinical correlates in hypertrophic cardiomyopathy

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    Hypertrophic cardiomyopathy (HCM) is a hereditary disease, which is caused by mutations in genech coding for proteins of carcomere of heart muscle. Disease is characterised by wide genotypic and fenotypic heterogeneity. Since the 90-th of 20-th century, when the first mutation responsible for HCM was identified, many research was done in detecting the correlation between mutant gene and clinical picture of the affected patient. As the first in Czech republic, we determined the frequencies of 4 most common genes in HCM in czech patients. We figured out, that spectrum of mutations is somehow different from another populations. We didn't find any correlation between specific gene mutation and morfologic and clinical characteristics of affected subjects. In our study with AT-1 receptor blocker - candesartan, we demostrated positive effect of candesartan on level of hypertrophy and function of the left heart ventricle and clinical symptoms of the patients with HCM

    Additional file 1: of The effects of storage temperature on PBMC gene expression

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    Supplemental data for Table 1. Provides a list of the 1,367 affected genes that demonstrated an increase or decrease in gene expression greater than 3-fold from 14 month cryopreserved PBMCs after they were thawed as compared to fresh PBMCs. It also contains a list of the 18 genes that were affected more than 3-fold when cryopreserved PBMCs were thawed after 14 months of storage at −150 °C versus −80 °C. (XLSX 191 kb
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