sj-docx-1-aat-10.1177_24723444241237302 – for Cellulose nanofiber as mask/personal protective equipment surface agent for enhanced anti-bacterial performance

sj-docx-1-aat-10.1177_24723444241237302 for Cellulose nanofiber as mask/personal protective equipment surface agent for enhanced anti-bacterial performance by Sophia Jackson and Jonathan Chen in AATCC Journal of Research</p

Additional file 2: of Practice patterns and outcomes for patients with node-negative hormone receptor-positive breast cancer and intermediate 21-gene Recurrence Scores

Figure S1. No difference in  overall survival by use of chemotherapy for patients diagnosed between 2006 and 2010 with median follow-up of 46.4 months. (TIFF 150 kb

Additional file 3: of Practice patterns and outcomes for patients with node-negative hormone receptor-positive breast cancer and intermediate 21-gene Recurrence Scores

Table S2. Utilization of chemotherapy by year. Chemotherapy use has been decreasing steadily over the years (trend from 2010 to 2013, p < 0.001). (DOCX 14 kb

Additional file 1: of Practice patterns and outcomes for patients with node-negative hormone receptor-positive breast cancer and intermediate 21-gene Recurrence Scores

Table S1. Chemotherapy receipt by age group, with age separated into three tiers using 35 and 50 years as clinically relevant thresholds. Chemotherapy receipt was significantly dependent on age group in this study (chi-square test, p < 0.001). (DOCX 15 kb

Additional file 4: of Practice patterns and outcomes for patients with node-negative hormone receptor-positive breast cancer and intermediate 21-gene Recurrence Scores

Table S3. Incidence of individual 21-gene Recurrence Scores from 11 to 25, and the comparative utilization of chemotherapy based on the score. (DOCX 14 kb

Quantification of AGEs using ELISA.

<p>Laminin was incubated with methylglyoxal and AGEs were quantified as methylglyoxal-hydro-imidazolone (MG-H1) adducts using competitive ELISA. Bar chart showing a concentration-dependent increase in MG-H1 adducts after treatment with methylglyoxal, n = 7.</p

AGE-modification of laminin results in disorganization actin cytoskeleton in rMC-1 cells.

<p>rMC-1 cells were propagated on AGE-modified laminin and stained for the phalloidin antibodies. The untreated cells exhibited continuous trans cytoplasmic actin filaments (white arrow). The treatment of rMC-1 cells on AGE-modified laminin resulted in disorganization of actin skeleton and the actin was mostly observed in clumps in the cytoplasm (yellow arrow), n = 3.</p

Decrease in colocalization of syntrophin and dystrophin on AGE-modified laminin.

<p>rMC-1 cells were propagated on AGE-modified laminin followed by staining with syntrophin and dystrophin antibodies, the images were taken using confocal microscopy. (<b>A</b>) Representative photomicrographs showing a decrease in the staining of dystrophin and clustering of syntrophin near cell membrane on AGE-modified laminin. Bar chart showing a Mander’s colocalization coefficient for (<b>B</b>) Syntrophin and (<b>C</b>) Dystrophin, n = 3.</p

Decrease in α-Dystroglycan complexes in rMC-1 cells propagated on AGE-modified laminin.

<p>rMC-1 cells were stained with α-dystroglycan antibodies followed by secondary staining using fluorescently conjugated antibodies. (<b>A</b>) Representative photomicrographs showing a decrease in α-dystroglycan expression in rMC-1 cells treated with AGE-modified laminin. (<b>B</b>) Quantification of α-dystroglycan complexes revealed a decrease in dystroglycan formation in rMC-1 cells propagated on AGE-modified laminin, n = 3.</p

AGE-modification of laminin leads to a decrease in Kir4.1 function.

<p>(<b>A</b>) Representative current traces of Kir4.1 currents under control and AGE-modified matrix (10 μM). Currents were elicited by applying 50-ms depolarizing potentials ranging from -140 mV to +30 mV from a holding potential of -60 mV (10-mV increment). Dash line indicates zero-current level. (<b>B</b>) Mean amplitude of the inward potassium currents elicited at -140 mV (control, n = 7; 10 μM, n = 9). p < 0.05.</p