17 research outputs found

    Cellular, cytokine and microbiologic characteristics of bronchoalveolar lavage from children with CSLD and the association (univariate regression analysis) between the BAL markers of inflammation and NTHi-driven IFN-γ production by blood mononuclear cells.

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    <p><sup>#</sup> β and confidence intervals reported as 100x concentration.</p><p>Cellular, cytokine and microbiologic characteristics of bronchoalveolar lavage from children with CSLD and the association (univariate regression analysis) between the BAL markers of inflammation and NTHi-driven IFN-γ production by blood mononuclear cells.</p

    Shows the univariate regression model of NTHi-driven IFN-γ production by blood mononuclear cells and IL-1β a) and IL-6 b) concentration in the bronchoalveolar lavage fluid.

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    <p>As the multivariate analyses showed that only IL-1γ and IL-6 were independently associated with the capacity for NTHi-driven IFN-γ production, other BAL mediators are not shown in the figure.</p

    Demographic characteristics and respiratory history of the cohort.

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    <p><sup>§</sup> scored according to a modified Bhalla scale as previously reported [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129517#pone.0129517.ref013" target="_blank">13</a>].</p><p>Demographic characteristics and respiratory history of the cohort.</p

    Cellular, cytokine and clinical characteristics of blood from children with CSLD and the association (univariate regression analysis) between the systemic markers of inflammation and NTHi-driven IFN-γ production by blood mononuclear cells.

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    <p><sup>#</sup> β and confidence intervals reported as 100x concentration.</p><p>Cellular, cytokine and clinical characteristics of blood from children with CSLD and the association (univariate regression analysis) between the systemic markers of inflammation and NTHi-driven IFN-γ production by blood mononuclear cells.</p

    Airway epithelial cell-induced changes in DC expression of Fcγ receptor genes.

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    <p>After 5 days of culture in the presence or absence of AEC, DC were sorted by flow cytometry. RNA from 15 independent experiments was extracted, and expression of Fc gamma receptor genes was determined using quantitative real-time PCR. **p<0.01; ***p<0.001.</p

    DC and airway epithelial cell expression of CD200R1 and CD200.

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    <p>After 5 days of culture in the presence or absence of AEC, cell surface expression of CD200R1 on DC and CD200 on AEC was determined by flow cytometry. Histograms from a representative experiment are shown. Similar changes were seen in all 6 experiments performed.</p

    Expression of type I interferon and IL-6 in AEC co-cultured with MDDC.

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    <p>AEC were cultured alone, in the presence or absence of GM-CSF + IL-4, or with MDDC. Following 5 days of culture RNA was extracted from sorted AEC and relative expression IFNα2 and IFNβ. *indicates p<0.05 relative to AEC alone (N = 6). IFNα protein was undetectable in culture supernatants. IL-6 protein data are from two experiments.</p

    Airway epithelial cell-induced changes in DC expression of selected immune response genes.

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    <p>(A). After 5 days of culture in the presence or absence of AEC, DC were sorted by flow cytometry. RNA from 15 independent experiments was extracted, and expression of immune response genes was determined using quantitative real-time PCR. **p<0.01; ***p<0.001. (B) Cell surface expression of B7-H1 and ICAM-1 was determined by flow cytometry. Cells staining with specific antibody and isotype control antibodies are shown. Histograms from a representative experiment are shown. Similar changes were seen in all 8 experiments performed.</p

    HRV16-induced expression of genes associated with the innate signalling pathways in PBMC pre-treated with the IFNAR blocking agent/decoy receptor B18R.

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    <p>PBMC derived from healthy controls were pre-treated with B18R (0.1 µg/mL) for 1 hour prior to stimulation with HRV16 (MOI = 5) for 24 hours. mRNA expression of IFNβ (A) TLR7 and TLR8 (B), STAT1 and IFNAR (C), interferon regulatory factors IRF1 and IRF7 (D) and, NFκB subunits p65, p50, p52, and IκBα and (E) were measured by qPCR. Results are displayed as the fold change in gene expression in stimulated cells, which is normalised to unstimulated cells; the dotted line at 1 represents no change in gene expression from the unstimulated cultures <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106501#pone.0106501-Pfaffl1" target="_blank">[25]</a>. Data are displayed as median and IQR. ns: not significant, *<i>p</i> value <0.05, **<i>p</i> value <0.01 using Mann-Whitney <i>U</i>-test comparing healthy (n = 20) to asthmatic (n = 22).</p

    Innate (24 hour) cytokine production.

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    <p>In vitro cytokine production by HC (open circles) and CSLD (filled circles) PBMC following 24 hour challenge with NTHi. Delta concentration (NTHi challenge minus nil challenge) with median and IQR.</p
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