Additional file 1: Figure S1. of Interferon-induced transmembrane protein 1 (IFITM1) overexpression enhances the aggressive phenotype of SUM149 inflammatory breast cancer cells in a signal transducer and activator of transcription 2 (STAT2)-dependent manner

Effects of IFITM1 knockdown on cell cycle and cell-cycle proteins in SUM149 cells. (PPT 212 kb

Utilization of mediation in the resolution of conflict within the framework of art schools in Prague in the school year 2012/2013

STAT1 and STAT2 knockdown in SUM149 using three different siRNAs. (PPT 185 kb

Additional file 2: Figure S2. of Everolimus downregulates estrogen receptor and induces autophagy in aromatase inhibitor-resistant breast cancer cells

Everolimus targets the phosphorylation of the PI3K/mTOR/Akt pathway at 48 and 72 h. MCF-7, MCF-7:5C and MCF-7:2A cells were seeded in 6-well plates and treated with 25, 50 or 100 nM everolimus or vehicle. Cells were harvested at 48 and 72 h and protein expression analyzed by western blot. Image represents three independent experiments. (PPT 674 kb

Additional file 1: Figure S1. of Everolimus downregulates estrogen receptor and induces autophagy in aromatase inhibitor-resistant breast cancer cells

Everolimus does not impact the proliferation or cycling of normal breast cells. (a) MCF10A cells were seeded in 24-well plates and treated with a range of everolimus doses in triplicate. The percentage of viable cells was determined after 72 h of everolimus treatment. (b) MCF10A cells were seeded in 6-well plates in single cell suspension and treated with 20 nM everolimus for 9 days. The number and size of clones was quantified and represents means from two independent experiments conducted in triplicate. (c) After 24, 48 and 72 h of 20 nM everolimus treatment in 6-well plates, MCF10A cells were subjected to cell cycle analysis. The percent of cells in G1 phase is highlighted. (PPT 507 kb

Supplementary Figures from Interaction Between MUC1 and STAT1 Drives IFITM1 Overexpression in Aromatase Inhibitor–Resistant Breast Cancer Cells and Mediates Estrogen-Induced Apoptosis

S1. E2 and GO-201 decrease IFITM1 expression. S2. Combination treatment with JAK/STAT inhibitor. S3. Alterations to IFITM1 expression upon MUC1 knockdown and Rux treatment. S4. Estrogen treatment reduces tumor size in Nude mice injected with MCF-7:5C AI-resistant cells in vivo. S5.Estrogen treatment reduces MUC 1, P-STAT1, and FITM 1 levels in Nude mice injected injected with MCF-7:5C AI-resistant cells in vivo. S6. TUNEL staining from AI-sensitive tumors. S7. MUC 1 staining intensities for ER+ breast cancer patients.</p