Table_1_Systematical Analysis of the Cancer Genome Atlas Database Reveals EMCN/MUC15 Combination as a Prognostic Signature for Gastric Cancer.DOCX

Digestive cancers-including gastric cancer (GC), colorectal cancer, hepatocellular carcinoma, esophageal cancer, and pancreatic cancer-accounted for 26% of cancer cases and 35% of cancer deaths worldwide in 2018. It is crucial and urgent to develop biomarkers for the diagnosis, prognosis, and therapeutic benefits of digestive cancers, especially for GC, since the incidence of GC is lower only than lung cancer in China, is hard to detect at an early stage, and is associated with poor prognosis. Mucins, glycoproteins encoded by MUC family genes, act as a part of a physical barrier in the digestive tract and participate in various signaling pathways. Some mucins have been used or proposed as biomarkers for carcinomas, such as MUC16 (CA125) and MUC4. However, there are no systematic investigations on the association of MUC family members with diagnoses and clinical outcomes even though relevant data have been largely accumulated in the past decade. By analyzing transcriptomic and clinical data of digestive cancer samples from TCGA involving colon adenocarcinoma (COAD), esophageal carcinoma (ESCA), liver hepatocellular carcinoma (LIHC), stomach adenocarcinoma (STAD), and pancreatic adenocarcinoma (PAAD), it was found that expressions levels of MUC15, MUC13, and MUC21 were individually associated with survival for digestive cancers, and high expressions of EMCN (MUC14) and MUC15 were correlated with poor survival for STAD. Cox regression analysis indicated the predictive power of an EMCN/MUC15 combination for overall survival (OS) of GC patients, which was validated on an independent dataset from GEO. EMCN/MUC15 correlated genes were identified to be enriched in cancer-related processes, such as vasculature development, mitosis, and immunity. Therefore, we propose that an EMCN/MUC15 combination could be a potential prognostic signature for gastric cancer.</p

MOESM1 of DRAP: a toolbox for drug response analysis and visualization tailored for preclinical drug testing on patient-derived xenograft models

Additional file 1. User’s guide for DRAP

Data_Sheet_1_Efficacy and safety of underwater endoscopic mucosal resection for ≤20 mm superficial non-ampullary duodenal epithelial tumors: Systematic review and meta-analysis.zip

Background and aimsSuperficial non-ampullary duodenal epithelial tumors (SNADETs) as a rare disease have gradually increased in recent years. Underwater endoscopic mucosal resection (UEMR) has emerged as a newly available option for the endoscopic resection of SNADETs. This study aimed to evaluate the efficacy and safety of UEMR for ≤20 mm SNADETs.MethodsA literature search was performed across multiple databases, including PubMed, Embase, Scopus, and Clinical trials for studies containing tumors ≤20 mm published from January 1, 2012, to August 8, 2022. Outcomes examined were the pooled rates of en bloc resection, R0 resection, adverse events, and recurrence. Subgroup analyses of the resection rate were conducted stratified by sample size and polyp size.ResultsA total of 10 studies with UEMR performed in a total of 648 tumors were included for analysis. The pooled rate of en bloc resection and R0 resection was 88.2% (95% confidence interval (CI): 82.1–93.2) and 69.1% (95% CI: 62.2–76.1), respectively. The results showed pooled rate of intraoperative bleeding rate was 2.9% (95% CI: 0–9.0), delayed bleeding rate was 0.9% (95% CI: 0.1–2), recurrence rate was 1.5% (95% CI: 0–4.9). In the subgroup analysis, R0 and en-bloc resection rates were significantly higher in ConclusionUnderwater endoscopic mucosal resection was an effective and safe technique for the optional treatment for ≤20 mm SNADETs, especially of Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022340578.</p

Isobaric (Vapor + Liquid) Equilibria for Propylene Carbonate with <i>para</i>-Xylene, <i>ortho</i>-Xylene, <i>meta</i>-Xylene, and Ethylbenzene at 101.33 kPa

At a pressure of 101.33 kPa, isobaric vapor–liquid equilibrium data for binary mixtures of propylene carbonate with para-xylene, ortho-xylene, meta-xylene, and ethylbenzene were obtained. Via a modified Othmer still apparatus and gas chromatography, the fractions of the vapor and liquid phases at equilibrium were measured. The experimental findings were fitted with the Wilson, NRTL, and UNIQUAC activity coefficient models, and the binary parameters of these models were calculated. The fitted models predicted the vapor–liquid equilibrium, and the calculated values agreed well with the experimental results. The Wisniak L-W and van Ness methods were used to authenticate the thermodynamic consistency of the equilibrium data

CD spectroscopic analysis of the β-sheet structure of PAP248-286.

<p>The spectra of PAP248-286 in the presence or absence of polyanions were calculated by subtracting the spectra of PBS or polyanions from those of PAP248-286 alone or PAP248-286+polyanions respectively. A, t = 12 h; B, t = 36 h; C, t = 60 h. The final peptide concentration of each sample was 120 µg/ml. The experiment was repeated once and similar result was obtained.</p

A brief schematic of the assays for testing the effect of polyanions on HIV-1 infection in the presence of PAP248-286 or SE-F.

<p>Polyanions at graded concentration was mixed with an indicated concentration of PAP248-286 or SE-F at an indicated interval with agitation as described in the “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059777#s2" target="_blank">Materials and Methods</a>”. After centrifugation, the supernatants were collected for testing the inhibitory activity of the unbounded polyanions on HIV-1 infection. The pellets containing the PAP248-286 (SEVI) - or SE-F-derived amyloid fibrils were re-suspended in 100 µl medium for testing the enhancing effect on HIV-1 infection.</p

Enhancement of HIV-1 infection of SE-F in the presence of polyanions.

<p>SE-F or PBS (as a control) was agitated with various polyanions at graded concentration for 5 h. The pellet portion of the mixture was mixed with R5 tropic (A) and X4 tropic viruses (B), respectively. The mixtures were added to TZM-b1 cells. After culture at 37°C for 3 h, the medium was replaced. Luciferase activity was detected 72 h later. The experiment was repeated once and similar results were obtained. Shown are average values (± standard deviations) of triplicate measurements of a representative experiment. *vs. HIV-1 in SE, <i>P</i><0.05 by <i>t</i>-test.</p

No significant cytotoxicity of SE-F and polyanions at the concentrations used in the present study.

<p>(<b>A</b>) Cytotoxicity of SE-F to different target cells. Seminal fluids (1∶500) and seminal pellet (1∶200) were incubated with TZM-bl cells or MT-2 cells respectively. After 48-h culture, cytotoxicity was determined by the XTT cell viability assay. The results were expressed as the mean of three different experiments. (<b>B</b>) Cytotoxicity of polyanions to different target cells. Various polyanions (50 µg/ml) were incubated with TZM-bl cells or MT-2 cells respectively. After 48-h culture, cytotoxicity was determined by the XTT cell viability assay. The results were expressed as the mean of three different experiments.</p

Effect of PAP248-286 on the antiviral activity of polyanionic candidate microbicides against laboratory-adapted HIV-1 strains^a.

a<p>IC₅₀s are means ± standard deviations (n = 3).</p

Effect of SE-F on the antiviral activity of polyanionic candidate microbicides against laboratory-adapted HIV-1 strains^a.

a<p>IC₅₀s are means ± standard deviations (n = 3).</p