The protocol described in this peer-reviewed article is published on protocols.io, dx.doi.org/10.17504/protocols.io.bpjdmki6.

The protocol is also available as a Supplementary Information for this publication. (PDF)</p

swapping_experiments_data

Raw data from symbiont swapping experiments involving ambrosia beetle Xylosandrus compactus and five species of ambrosiella fungi

Beetle_tree_and_alignment

Alignment and tree for for ambrosia Xyleborini ambrosia beetles in Nexus format

Data for: Experimental evidence that fungal symbionts of bark and ambrosia beetles suppress decomposition of pine sapwood by competing with wood decay fungi.

Full SEM images from multi panel figure 3 in the manuscript

Data for: Experimental evidence that fungal symbionts of bark and ambrosia beetles suppress decomposition of pine sapwood by competing with wood decay fungi.

Supplemental Figures S1 and S2

Video supplement V2 from A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts

Extraction of Xylosandrus compactus mycangium contents. Beetles were mounted live on ultrafine pins. Fungal masses were extracted from mycangia by applying gentle pressure to the scutellum. This technique allows a sanitary removal of the complete living fungal mass for quantification of viable spores by serial dilution and culture. Mycangia that did not contain a fungal mass were verified by complete eversion of the empty mycangium

Supplemental Tables S1 and S2 from A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts

Tables with source information and GenBank accession numbers for sequences used in phylogenetic analyses

Supplemental methods from A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts

Additional methods and material

Supplemental Tables S1 and S2 from A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts

Tables with source information and GenBank accession numbers for sequences used in phylogenetic analyses

Video supplement V2 from A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts

Extraction of Xylosandrus compactus mycangium contents. Beetles were mounted live on ultrafine pins. Fungal masses were extracted from mycangia by applying gentle pressure to the scutellum. This technique allows a sanitary removal of the complete living fungal mass for quantification of viable spores by serial dilution and culture. Mycangia that did not contain a fungal mass were verified by complete eversion of the empty mycangium