The genetic distance between different populations based on mtDNA CR sequence.^*.

*<p>MtDNA CR genetic distances among different population were obtained by MEGA Version Data are presented by intraspecific or interspecific congeneric K2P-distances.</p

Tilapia species <i>S. melanotheron</i> and <i>T. rendalli</i>.

<p>Tilapia species <i>S. melanotheron</i> and <i>T. rendalli</i>.</p

The genetic distance between different populations based on mt DNA COI sequence.^*.

*<p>MtDNA CR genetic distances among different population were obtained by MEGA Version4. Data are presented by intraspecific or interspecific congeneric K2P-distances.</p

Phylogenetic tree constructed with tilapia mtDNA CR sequences using the Kimura two-parameter distance model.

<p>Bootstrap values greater than 50 are shown. A1, B1, C1, C2, D1, E1, F1, G1, H1, I1, I2, J1, K1, L1, L2, and L3 are the representative mtDNA CR sequence from different fish populations. <i>O. niloticu</i> x <i>O. mossambic</i> (accession #AY833481), <i>O. mossambic</i> (accession #AY833459), <i>O. urolepis</i> (accession #AF296467), <i>O. aureus</i> (accession #AF328851), <i>O. niloticus</i> (accession #AF485083), <i>S. melanotheron</i> (accession #AF484717), and <i>T. rendalli</i> (accession #AF328854) are reference tilapia species, and the accession numbers were obtained from the NCBI database.</p

Comparisons between <i>O. niloticus</i> present in Hawaii and <i>O. niloticus</i> in Egypt.

<p>Comparisons between <i>O. niloticus</i> present in Hawaii and <i>O. niloticus</i> in Egypt.</p

Genetic variability in captive and wild populations.^*.

*<p>N: the number of sequences; Pi: nucleotide diversity within the population; H: number of different sequences types; K: average number of nucleotide differences within the population.</p

Alignments of the variable sites of mtDNA CR sequences from the representative fish populations.

<p>Dashes indicate indels introduced for optimal alignment, and the asterisks at the bottom of the figure indicate the consensus nucleotide. A1, B1, C1, C2, D1, E1, F1, G1, H1, I1, I2, J1, K1 and L1 are the representative mtDNA CR sequence types from different fish populations.</p

Identifications of Captive and Wild Tilapia by mtDNA CR Sequence.

<p>Identifications of Captive and Wild Tilapia by mtDNA CR Sequence.</p

Phylogenetic tree constructed with tilapia mtDNA CR sequences by PHYLIP program.

<p>A1, B1, C1, C2, D1, E1, F1, G1, H1, I1, I2, J1, K1, L1, L2, and L3 are the representative mtDNA CR sequence from different fish populations. <i>O. niloticu</i> x <i>O. mossambic</i> (O.n x O.m, accession #AY833481), <i>O. mossambic</i> (O.moss, accession #AY833459 and O.moss 2, accession # AF328843), <i>O. urolepis</i> (accession #AF296467), <i>O. aureus</i> (accession #AF328851), <i>O. niloticus</i> (O. nilo, accession #AF485083), <i>S. melanotheron</i> (S. melan accession #AF484717 and S. melan 2 accession # AF296493), and <i>T. rendalli</i> (T. rend, accession #AF328854) are reference tilapia species, and the accession numbers were obtained from the NCBI database.</p

DNA binding protein assay.

<p>LvDBP23 (A) and GFP (B) proteins binding with dsDNA cellulose and ssDNA cellulose bead in different total protein amount of 100, 200, 400 µg. P: only recombinant protein without DNA in the reaction. Both proteins were detected by anti-His antibody.</p