Swelling/Deswelling-Induced Reversible Surface Wrinkling on Layer-by-Layer Multilayers

Layer-by-layer (LbL) multilayer film is incorporated in the fabrication of a film/substrate system for the investigation of swelling/deswelling-induced wrinkle evolution for the first time. As one typical example, hydrogen-bonded (PAA/PEG)_<i>n</i> (PAA, poly­(acrylic acid); PEG, poly­(ethylene glycol)) is deposited on a poly­(dimethylsiloxane) (PDMS) substrate via the LbL technique. Heating treatment causes the covalent cross-linking reaction to occur in the H-bonded multilayers with simultaneously spontaneous formation of labyrinth wrinkles. Subsequent water immersion leads to the evolution of a series of the swelling-sensitive wrinkles in the thermally cross-linked (PAA/PEG)_<i>n</i>/PDMS bilayer, ranging from initial labyrinth wrinkles (a) to an intermediate smooth wrinkle-free state (b), hexagonally arranged dimples (c), and the later-segmented labyrinth patterns (d). Upon deswelling by reheating of the swollen bilayer, the reverse wrinkle evolution happens via the process of d → b, or d → b → a, or c → b, or c → b → a, which is dependent on the reheating temperature and the swelling-induced pattern. We investigate the influences of experimental conditions on the swelling kinetics and the resulting wrinkle evolution, which include the thickness of (PAA/PEG)_<i>n</i>, the additionally deposited outermost layer (e.g., Pt and polystyrene), and the swelling solution pH. The involved mechanism has been discussed from the viewpoint of the relation between the wrinkling behavior and the swelling/deswelling-induced stress state. The results indicate that the combined strategy of LbL assembly with the introduction of additional layers endows us with considerable freedom to fabricate multifunctional film/substrate systems and to tune the instability-driven patterns for advanced properties and extended applications

Biosynthesis, Characterization, and Hemostasis Potential of Tailor-Made Poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) Produced by Haloferax mediterranei

We report the biosynthesis of poly­(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) random copolymers (R-PHBV) or higher-order copolymers (O-PHBV) in Haloferax mediterranei, with adjustable 3-hydroxyvalerate (3HV) incorporation by cofeeding valerate with glucose. Their microchemical structure, molecular weight and its distribution, and thermal and mechanical properties were characterized by NMR, GPC, DSC, TGA, and universal testing machine, respectively. ¹³C NMR studies showed that O-PHBV copolymers consisted of short segments of PHB and PHV covalently linked together with random PHBV segments. Consistently, two <i>T</i>_g were observed in the DSC curves of O-PHBV. The “blocky” feature of O-PHBV enhanced crystallinity percentages and improved Young’s modulus. Notably, the film of one O-PHBV copolymer, O-PHBV-1, showed unique foveolar cluster-like surface morphology with high hydrophobicity and roughness, as characterized using static contact angle and SEM and AFM analyses. It also exhibited increased platelet adhesion and accelerated blood clotting. The excellent hemostatic properties endow this copolymer with great potential in wound healing

Swelling/Deswelling-Induced Reversible Surface Wrinkling on Layer-by-Layer Multilayers

Layer-by-layer (LbL) multilayer film is incorporated in the fabrication of a film/substrate system for the investigation of swelling/deswelling-induced wrinkle evolution for the first time. As one typical example, hydrogen-bonded (PAA/PEG)_<i>n</i> (PAA, poly­(acrylic acid); PEG, poly­(ethylene glycol)) is deposited on a poly­(dimethylsiloxane) (PDMS) substrate via the LbL technique. Heating treatment causes the covalent cross-linking reaction to occur in the H-bonded multilayers with simultaneously spontaneous formation of labyrinth wrinkles. Subsequent water immersion leads to the evolution of a series of the swelling-sensitive wrinkles in the thermally cross-linked (PAA/PEG)_<i>n</i>/PDMS bilayer, ranging from initial labyrinth wrinkles (a) to an intermediate smooth wrinkle-free state (b), hexagonally arranged dimples (c), and the later-segmented labyrinth patterns (d). Upon deswelling by reheating of the swollen bilayer, the reverse wrinkle evolution happens via the process of d → b, or d → b → a, or c → b, or c → b → a, which is dependent on the reheating temperature and the swelling-induced pattern. We investigate the influences of experimental conditions on the swelling kinetics and the resulting wrinkle evolution, which include the thickness of (PAA/PEG)_<i>n</i>, the additionally deposited outermost layer (e.g., Pt and polystyrene), and the swelling solution pH. The involved mechanism has been discussed from the viewpoint of the relation between the wrinkling behavior and the swelling/deswelling-induced stress state. The results indicate that the combined strategy of LbL assembly with the introduction of additional layers endows us with considerable freedom to fabricate multifunctional film/substrate systems and to tune the instability-driven patterns for advanced properties and extended applications

Genetic linkage map of the male parent.

<p>Note: Markers in bold and italic fonts are SSR markers; for marker ending with a letter of “R”, the corresponding marker is repulsion linkage phase; markers with significant segregation distortion are indicated with “*” when <i>P</i>≤0.05, or “**” when <i>P</i>≤0.01.</p

AFLP marker distribution on the paternal map.

<p>Note:</p><p>“*” indicates significance at α = 0.05;</p><p>“**” indicates significance at α = 0.01; the probabilities for clustering or dispersal of AFLP markers(m_<i>i</i>≥<i>λ</i>_i or m_<i>i</i>≤<i>λ</i>_i) were evaluated by using a two-tailed cumulative Poisson calculator (<i>P</i> ≤ 0.025 is significant at α = 0.05).</p><p>AFLP marker distribution on the paternal map.</p

Toxic Effects and Molecular Mechanism of Different Types of Silver Nanoparticles to the Aquatic Crustacean <i>Daphnia magna</i>

Silver nanoparticles (AgNPs) have been assessed to have a high exposure risk for humans and aquatic organisms. Toxicity varies considerably between different types of AgNPs. This study aimed to investigate the toxic effects of AgNPs with different particle sizes (40 and 110 nm) and different surface coatings (sodium citrate and polyvinylpyrrolidone, PVP) on <i>Daphnia magna</i> and their mechanisms of action. The results revealed that the citrate-coated AgNPs were more toxic than PVP-coated AgNPs and that the 40 nm AgNPs were more toxic than the 110 nm AgNPs. Transcriptome analysis further revealed that the toxic effects of AgNPs on <i>D. magna</i> were related to the mechanisms of ion binding and several metabolic pathways, such as the “RNA polymerase” pathway and the “protein digestion and absorption” pathway. Moreover, the principal component analysis (PAC) results found that surface coating was the major factor that determines the toxicities compared to particle size. These results could help us better understand the possible mechanism of AgNP toxicity in aquatic invertebrates at the transcriptome level and establish an important foundation for revealing the broad impacts of nanoparticles on aquatic environments

Profiles of the LOD score for SDL declaration throughout the male and female maps.

<p>2a. The male map; 2b. The female map. Note: Y-axis shows the LOD score, and X-axis displays the accumulated genetic length in order of the linkage group numbers. The Rome digital on the top of the figure are the linkage group identities.</p

Microarray-Based Analysis of Gene Expression in <i>Lycopersicon esculentum</i> Seedling Roots in Response to Cadmium, Chromium, Mercury, and Lead

The effects of heavy metals in agricultural soils have received special attention due to their potential for accumulation in crops, which can affect species at all trophic levels. Therefore, there is a critical need for reliable bioassays for assessing risk levels due to heavy metals in agricultural soil. In the present study, we used microarrays to investigate changes in gene expression of <i>Lycopersicon esculentum</i> in response to Cd-, Cr-, Hg-, or Pb-spiked soil. Exposure to ¹/₁₀ median lethal concentrations (LC₅₀) of Cd, Cr, Hg, or Pb for 7 days resulted in expression changes in 29 Cd-specific, 58 Cr-specific, 192 Hg-specific and 864 Pb-specific genes as determined by microarray analysis, whereas conventional morphological and physiological bioassays did not reveal any toxicant stresses. Hierarchical clustering analysis showed that the characteristic gene expression profiles induced by Cd, Cr, Hg, and Pb were distinct from not only the control but also one another. Furthermore, a total of three genes related to “ion transport” for Cd, 14 genes related to “external encapsulating structure organization”, “reproductive developmental process”, “lipid metabolic process” and “response to stimulus” for Cr, 11 genes related to “cellular metabolic process” and “cellular response to stimulus” for Hg, 78 genes related to 20 biological processes (e.g., DNA metabolic process, monosaccharide catabolic process, cell division) for Pb were identified and selected as their potential biomarkers. These findings demonstrated that microarray-based analysis of <i>Lycopersicon esculentum</i> was a sensitive tool for the early detection of potential toxicity of heavy metals in agricultural soil, as well as an effective tool for identifying the heavy metal-specific genes, which should be useful for assessing risk levels due to heavy metals in agricultural soil

The SDLs detected based on the established genetic maps.

<p>Note: Effect span indicates the genetic length that a SDL would cause segregation distortion of makers within the region. “+” represents segregation distortion skews to more visible alleles for markers in the affected genome region, and “-” indicates segregation distortion skews to less visible alleles for markers in the affected genome region.</p><p>The SDLs detected based on the established genetic maps.</p

Table_4_Integrating PANoptosis insights to enhance breast cancer prognosis and therapeutic decision-making.xlsx

BackgroundDespite advancements, breast cancer outcomes remain stagnant, highlighting the need for precise biomarkers in precision medicine. Traditional TNM staging is insufficient for identifying patients who will respond well to treatment.MethodsOur study involved over 6,900 breast cancer patients from 14 datasets, including in-house clinical data and single-cell data from 8 patients (37,451 cells). We integrated 10 machine learning algorithms in 55 combinations and analyzed 100 existing breast cancer signatures. IHC assays were conducted for validation, and potential immunotherapies and chemotherapies were explored.ResultsWe pinpointed six stable Panoptosis-related genes from multi-center cohorts, leading to a robust Panoptosis-model. This model outperformed existing clinical and molecular features in predicting recurrence and mortality risks, with high-risk patients showing worse outcomes. IHC validation from 30 patients confirmed our findings, indicating the model’s broader applicability. Additionally, the model suggested that low-risk patients benefit more from immunotherapy, while high-risk patients are sensitive to specific chemotherapies like BI-2536 and ispinesib.ConclusionThe Panoptosis-model represents a major advancement in breast cancer prognosis and treatment personalization, offering significant insights for effectively managing a wide range of breast cancer patients.</p